Proteolytic Activity of Crude Stem Extracts from Normal and Tumour Tissues of Plants

The activities of twelve enzymes were studied in Acer psendoplatanum L. cells grown in suspension cultures for 6, 12, 19 and 22 days. The enzyme activities of cells (6 and 12 days) in the phase of rapid cell division were rather different from that of cells in the stationary phase (19, 22 days). The activity of aldolasc was highest at the first two stages of growth, when the amount of RNA and protein per cell was highest, and after this decreased steeply. The curves of specific activities of several phosphatases (glucosc‐6‐phosphatasc, α‐glycerophosphatase, acid phosphatase and ATPase) and ribonuclease were almost the reverse of the curve of aldolase, showing a minimum in 12‐day‐old cells and a maximum in cells 19 and 22 days old. The activity of peroxidase was also high in ageing cells. Glutamate: oxaloacetate transaminase and aminopeptidases (leucine and alanine) had synchronous maxima (6 and 19 days) of specific activity but the activity of aminopeptidases decreased gradually during ageing of the suspension, if the enzyme activities are calculated per 107 cells. Glutamate: pyruvate transaminasc activity was very low and no dcoxyribonuclease activity could be detected.

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“…Glyeolysis is the obvious source of energy for Physm. Plant., 23,1970 mitosis (Rapkine 1931, Warburg 1956, Paul 1959. 1961.…”

Section: Resultsmentioning

confidence: 99%