Proteolysis of elastin-ligand complexes. Stimulation of elastase digestion of insoluble elastin by sodium dodecyl sulfate

Kagan, Herbert M.; Crombie, George; Jordan, Robert E.; Lewis, William; Franzblau, Carl

doi:10.1021/bi00768a014

Cited by 82 publications

(41 citation statements)

References 8 publications

(8 reference statements)

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“…Enzymic activity was maximal at an SDS:elastin ratio of 1 :4, whereas increased concentrations of SDS inhibited lysis. At the optimal ratio the activity of macrophage elastase was at least 20 times that in the absence of SDS . Pancreatic elastase was affected much less by the addition of SDS to the elastin (not shown) .…”

Section: Resultsmentioning

confidence: 94%

“…Elastin binds hydrophobic ligands such as SDS strongly and both porcine pancreatic elastase (20,21) and human spleen elastase (10) are more active against elastin-ligand complexes . Accordingly, the effect of SDS on elastinolysis by macrophage CM was first examined .…”

Section: Resultsmentioning

confidence: 99%

“…The interaction between pancreatic elastase and elastin is electrostatic and high salt concentrations effectively depress enzyme activity (20) . Since CM contained physiological salt concentrations and other preparations had varying salt concentrations, the effect of salt on elastinolysis by macrophage CM was examined .…”

Section: Resultsmentioning

confidence: 99%

“…At 40°C the area of the zone of lysis increased linearly for 80-100 h and then increased somewhat more slowly for up to 24 days, with a reasonably linear relationship between area of zone of lysis and log time. Readings were taken three times at 16,20,24,40,48, or 72 h and compared with standard curves for each time . The ratio of activities of different samples therefore remained constant with assay time .…”

Section: Relationship Of Elastinolysis To Enzyme Concentrationmentioning

confidence: 99%

“…Sodium dodecyl sulfate (SDS :elastin ratio 1:4, by weight) was added to the mixture and sodium azide (0 .05%) included to prevent bacterial growth . Aliquots (11 ml) were poured onto 8 x 8 cm plates on a leveling table and the plates kept overnight to allow the SDS to bind to the elastin (20,21). Plates were stored in a moist chamber at 4°C before use .…”

mentioning

confidence: 99%

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Elastase secretion by stimulated macrophages. Characterization and regulation.

Werb¹,

Gordon²

1975

The Journal of Experimental Medicine

480

196

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Thioglycolate-stimulated mouse peritoneal macrophages secrete a Proteinase which degrades insoluble elastin. There is little elastase activity in cell lysates but the bulk of the enzyme accumulates extracellularly during culture in serum-free medium. The secretion of elastase is sustained for over 12 days in culture and continued secretion of elastase requires protein synthesis. Unstimulated macrophages secrete very little elastase activity but can be triggered to secrete higher levels of this enzyme by phagocytosis and intracellular storage of latex particles. The macrophages elastase is a distinctive proteinase differing from the elastases of pancreas and granulocytes and is distinct from the other secreted proteinases of macrophages, namely, collagenase and plasminogen activator. The macrophages elastase is a serine proteinase and is inhibited by di-isopropyl phosphoro-fluoridate, ovoinhibitor, EDTA, dithiothretiol, and serum. Its activity is little affected by soybean trypsin inhibitor, turkey ovomucoid and chloromethyl ketones derived from tosyl lysine, tosyl phenylalanine, and acetyltetra alanine. Hydrolysis by macrophage elastase of chromogenic ester substrates for pancreatic elastase could not be detected. Elastase secretion by stimulated macrophages exceeds that by primary and established fibroblast cell strains. It is likely that elastase secretion by macrophages plays a major role in the pathogenesis of chronic destructive pulmonary diseases such as emphysema.

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Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Relationship Of Elastinolysis To Enzyme Concentrationmentioning

confidence: 99%

mentioning

confidence: 99%

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Elastase secretion by stimulated macrophages. Characterization and regulation.

Werb¹,

Gordon²

1975

The Journal of Experimental Medicine

480

196

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Interaction between fatty acid salts and elastin: Kinetics, absorption equilibrium, and consequences for elasticity

Vreeswijk

Lyklema

1999

Biopolymers

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Effects of lipids on elastin's viscoelastic properties

Lillie

Gosline

2002

Biopolymers

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Sodium dodecyl sulfate (SDS) was used as a model lipid to identify the molecular basis of possible lipid-induced changes in the viscoelastic behavior of arterial elastin. The chemical composition of the elastin network and the interfibrillar space was calculated from the chemical content of the elastin sample and its swelling behavior. Viscoelastic behavior was measured in aqueous SDS and in SDS plus 1 M sucrose, a deswelling agent. Viscoelastic behavior was also measured in sucrose and potassium thiocyanate solutions to identify the effects of swelling and of changes in network composition exclusive of any direct SDS effects. The hydration of the elastin network decreased at low SDS levels and increased at higher SDS levels. The elastin was stiffer in the dehydrated network and less stiff in the hydrated network. However, once the degree of hydration exceeded that of elastin in pure water, no further decrease in stiffness was obtained despite continued increase in swelling. The stiffness of the network could be accounted for entirely by changes in network hydration. There was no evidence that SDS had any effect on elastin's conformation. We predict that arterial lipids will interact with elastin in a similar way and will have only small effects on elastin's viscoelastic behavior.

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Proteolysis of elastin-ligand complexes. Stimulation of elastase digestion of insoluble elastin by sodium dodecyl sulfate

Cited by 82 publications

References 8 publications

Elastase secretion by stimulated macrophages. Characterization and regulation.

Elastase secretion by stimulated macrophages. Characterization and regulation.

Interaction between fatty acid salts and elastin: Kinetics, absorption equilibrium, and consequences for elasticity

Effects of lipids on elastin's viscoelastic properties

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