Proteoliposomes as matrix vesicles' biomimetics to study the initiation of skeletal mineralization

Simão, Adenilso da Silva; Yadav, Manisha; Ciancaglini, Pietro; Millán, José Luis

doi:10.1590/s0100-879x2010007500008

Cited by 30 publications

(54 citation statements)

References 55 publications

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“…In addition, proteins tend to accommodate among the lipid groups, creating lipid-protein domains and forming the proteoliposomes. By changing the type and proportion of the lipids, incubation time, method used for detergent removal or even the velocity by which the detergent is removed, different vesicular systems can be obtained, varying in the type and quantity of proteins that can be reconstituted (Bolean et al 2010(Bolean et al , 2011Camolezi et al 2002;Daghastanli et al 2004;de Lima Santos et al 2005;Ierardi et al 2002;Rigos et al 2008Rigos et al , 2010Simao et al 2010a), 2015. Anchored membrane proteins are a class of proteins with a covalently bound lipid or alkyl chain; this chain provides a hydrophobic moiety that allows attachment to the membrane and plays specific functions in cells (Yeagle 1993).…”

Section: What Are the Biomimetic Systems Of Mvs?mentioning

confidence: 99%

“…Anchored membrane proteins are a class of proteins with a covalently bound lipid or alkyl chain; this chain provides a hydrophobic moiety that allows attachment to the membrane and plays specific functions in cells (Yeagle 1993). For this class of proteins, the method of incorporation via direct insertion guarantees that the protein is associated with the external leaflet of the liposome's membrane (Bolean et al 2010(Bolean et al , 2011Camolezi et al 2002;Daghastanli et al 2004;de Lima Santos et al 2005;Ierardi et al 2002;Rigos et al 2008Rigos et al , 2010Simao et al 2010aSimao et al , 2015. The incorporation of the protein/enzyme is time-dependent and the incorporation yield depends on the lipid composition used to form the vesicles .…”

Section: What Are the Biomimetic Systems Of Mvs?mentioning

confidence: 99%

“…Biophysical and biochemical characterization of biomimetic models Biophysical tools applied to biomineralization studies A large number of studies have been carried out using proteoliposomes as chondrocyte-and osteoblast-derived MVs biomimetics (Andrade et al 2016;Bolean et al 2010Bolean et al , 2011Bolean et al , 2015Bolean et al , 2017Ciancaglini et al 2010;Favarin et al 2017;Simao et al 2010aSimao et al , b, 2013.…”

Section: What Are the Biomimetic Systems Of Mvs?mentioning

confidence: 99%

“…The size of a dipalmitoylphosphatidylcholine (DPPC) proteoliposome harboring TNAP is around 300 nm, as determined by dynamic light scattering (Bolean et al 2010;Simao et al 2010a). It is thus comparable to the median size of a natural MV Ciancaglini et al 2006) and can adequately serve as a vesicular mimetic system to examine TNAP function in the context of a lipid membrane environment that mimics the MV environment.…”

Section: What Are the Biomimetic Systems Of Mvs?mentioning

confidence: 99%

“…It is thus comparable to the median size of a natural MV Ciancaglini et al 2006) and can adequately serve as a vesicular mimetic system to examine TNAP function in the context of a lipid membrane environment that mimics the MV environment. Electron microscopy of empty DPPC liposomes and TNAP-proteoliposomes showed that enzyme reconstitution did not affect the morphology of the liposomes (Simao et al 2010a). Garcia et al (2015) used electron spin resonance measurements along with spin labeled phospholipids to probe the possible dynamic changes prompted by the interaction of GPIanchored TNAP with model membranes.…”

Section: What Are the Biomimetic Systems Of Mvs?mentioning

confidence: 99%

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Biophysical aspects of biomineralization

et al. 2017

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During the process of endochondral bone formation, chondrocytes and osteoblasts mineralize their extracellular matrix (ECM) by promoting the synthesis of hydroxyapatite (HA) seed crystals in the sheltered interior of membranelimited matrix vesicles (MVs). Several lipid and proteins present in the membrane of the MVs mediate the interactions of MVs with the ECM and regulate the initial mineral deposition and posterior propagation. Among the proteins of MV membranes, ion transporters control the availability of phosphate and calcium needed for initial HA deposition. Phosphatases (orphan phosphatase 1, ectonucleotide pyrophosphatase/ phosphodiesterase 1 and tissue-nonspecific alkaline phosphatase) play a crucial role in controlling the inorganic pyrophosphate/inorganic phosphate ratio that allows MVmediated initiation of mineralization. The lipidic microenvironment can help in the nucleation process of first crystals and also plays a crucial physiological role in the function of MVassociated enzymes and transporters (type III sodiumdependent phosphate transporters, annexins and Na + /K + ATPase). The whole process is mediated and regulated by the action of several molecules and steps, which make the process complex and highly regulated. Liposomes and proteoliposomes, as models of biological membranes, facilitate the understanding of lipid-protein interactions with emphasis on the properties of physicochemical and biochemical processes. In this review, we discuss the use of proteoliposomes as multiple protein carrier systems intended to mimic the various functions of MVs during the initiation and propagation of mineral growth in the course of biomineralization. We focus on studies applying biophysical tools to characterize the biomimetic models in order to gain an understanding of the importance of lipid-protein and lipid-lipid interfaces throughout the process.Keywords Lipid microenvironment . Biomineralization . Matrix vesicles . Liposome . Proteoliposome . Hydroxyapatite Mineralization process and the biogenesis of matrix vesiclesMineralization of cartilage and bone occurs by a series of physicochemical and biochemical processes that together facilitate the deposition of calcium phosphate. The deposited calcium phosphate is subsequently converted into hydroxyapatite (HA) [Ca 10 (PO 4 ) 6 (OH) 2 ] in specific areas of the extracellular matrix (ECM). Various experiments have revealed the presence of HA crystals both inside and outside collagen fibrils in the ECM (McNally et al. 2012) and also within the

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Section: What Are the Biomimetic Systems Of Mvs?mentioning

confidence: 99%

Section: What Are the Biomimetic Systems Of Mvs?mentioning

confidence: 99%

Section: What Are the Biomimetic Systems Of Mvs?mentioning

confidence: 99%

Section: What Are the Biomimetic Systems Of Mvs?mentioning

confidence: 99%

Section: What Are the Biomimetic Systems Of Mvs?mentioning

confidence: 99%

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Biophysical aspects of biomineralization

et al. 2017

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Different compact hybrid Langmuir–Blodgett‐film coatings modify biomineralization and the ability of osteoblasts to grow

et al. 2018

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Calcium phosphates (CaPs) are biomaterials widely used in tissue regeneration with outstanding biological performance. Although the tremendous improvements achieved in CaP's materials research over the years, their interaction with physiological environments still need to be fully understood. The aim of this study is to explore a biomimetic Langmuir-Blodgett (LB) membrane to template the growth of hydroxyapatite (HAp) coatings on Ti surfaces and the ability of these coatings in inducing biomineralization by osteoblasts cultured in vitro. Changing the phospholipids (i.e., dihexadecyl phosphate (DHP) or octadecylphosphonic acid (OPA)), we also tuned the surface Ca concentration. This structural feature gave rise to different LB-hybrid surfaces where the concentration of Ca in the OPA/HAp was higher than the concentration of Ca in DHP/HAp coating. The higher Ca amount on OPA/HAp coatings, allied to the physical-chemical features, lead to different responses on osteoblasts, stimulating or inhibiting the natural biomineralization. The OPA/HAp coating caused a delay in the osteoblast proliferation as indicated by the decrease in the cell viability at the 7th culture day. Improved cell differentiation triggered by the DHP/HAp coating resulted in higher osteoblast biomineralization. The present data underscore that besides both coatings being composed by HAp, the final interfacial composition and physical-chemical properties influence differently the osteoblast behavior. Although the best osteoblast's viability was found to OPA/HAp, our dataset attested that DHP/HAp induced mineralization more effectively than that. This unexpected finding highlight the importance of deeply understanding the biomaterial interface and suggest a promising approach to the design of biofunctional LB-based coatings with tunable properties. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2524-2534, 2018.

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Effects of pH on the Production of Phosphate and Pyrophosphate by Matrix Vesicles’ Biomimetics

et al. 2013

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During endochondral bone formation, chondrocytes and osteoblasts synthesize and mineralize the extracellular matrix through a process that initiates within matrix vesicles (MVs) and ends with bone mineral propagation onto the collagenous scaffold. pH gradients have been identified in the growth plate of long bones, but how pH changes affect the initiation of skeletal mineralization is not known. Tissue-nonspecific alkaline phosphatase (TNAP) degrades extracellular inorganic pyrophosphate (ePPi), a mineralization inhibitor produced by ectonucleotide pyrophosphatase/ phosphodiesterase-1 (NPP1), while contributing Pi from ATP to initiate mineralization. TNAP and NPP1, alone or combined, were reconstituted in dipalmitoylphosphatidylcholine (DPPC) liposomes to mimic the microenvironment of MVs. The hydrolysis of ATP, ADP, AMP and PPi was studied at pH 8 and 9 and compared to the data determined at pH 7.4. While catalytic efficiencies in general were higher at alkaline pH, PPi hydrolysis was maximal at pH 8 and indicated a preferential utilization of PPi over ATP, at pH 8 versus 9. In addition, all proteoliposomes induced mineral formation when incubated in a synthetic cartilage lymph (SCL) containing 1 mM ATP as substrate and amorphous calcium phosphate (ACP) or calciumphosphate- phosphatidylserine complexes (PS-CPLX) as nucleators. Propagation of mineralization was significantly more efficient at pHs 7.5 and 8 than at pH 9. Since a slight pH elevation from 7.4 to 8 promotes considerably more hydrolysis of ATP, ADP and AMP primarily by TNAP, this small pH change facilitates mineralization, especially via upregulated PPi hydrolysis by both NPP1 and TNAP, further elevating the Pi/PPi ratio, thus enhancing bone mineralization.

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Proteoliposomes as matrix vesicles' biomimetics to study the initiation of skeletal mineralization

Cited by 30 publications

References 55 publications

Biophysical aspects of biomineralization

Biophysical aspects of biomineralization

Different compact hybrid Langmuir–Blodgett‐film coatings modify biomineralization and the ability of osteoblasts to grow

Effects of pH on the Production of Phosphate and Pyrophosphate by Matrix Vesicles’ Biomimetics

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