Proteoglycans contribute locally to swelling, but globally to compressive mechanics, in intact cervine medial meniscus

Michalek, Arthur J.; Kuxhaus, Laurel; Jaremczuk, Daniel; Zaino, Nicole L

doi:10.1016/j.jbiomech.2018.04.023

Cited by 12 publications

(8 citation statements)

References 23 publications

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“…Values of crimping period in the present study are of about the same magnitude as reported earlier 18,19 . Michalek et al, reported values of crimp period in the lamellar layer of deer menisci of around 20e30 mm in healthy controls and around 10 mm in enzymatically degraded samples 18 . Values of crimping length, equaling half the period, of 3.7e9.4 mm was reported in human meniscal attachments by Villegas et al 19 .…”

Section: Discussionsupporting

confidence: 90%

Phase-contrast enhanced synchrotron micro-tomography of human meniscus tissue

Einarsson¹,

Pierantoni²,

Novák³

et al. 2022

Osteoarthritis and Cartilage

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Section: Discussionsupporting

confidence: 90%

Phase-contrast enhanced synchrotron micro-tomography of human meniscus tissue

Einarsson¹,

Pierantoni²,

Novák³

et al. 2022

Osteoarthritis and Cartilage

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“…According to our strategy, overexpression of IGF1 in 3CI is only maintained for 7 days (Figure 4). Due to this, the observed effect at the transcriptional level after 28 days of culture is not appreciable; nonetheless, our data demonstrated that this design has at the protein level a good balance between Col-I and Col-II expression, which results in a structure similar to that of the native cartilage (Figure 6(b)) as well as enhanced expression of proteoglycans (Figure 7(a)) whose influence on the mechanical features is well known [52]. Furthermore, it is shown that the extracellular matrix is dense and well-structured and lacks fibrocartilage compared to the group not transduced with Ad-IGF1 (2CI).…”

Section: Discussionmentioning

confidence: 67%

A Bioactive Cartilage Graft of IGF1-Transduced Adipose Mesenchymal Stem Cells Embedded in an Alginate/Bovine Cartilage Matrix Tridimensional Scaffold

Moncada‐Saucedo

Marino-Martínez

Lara‐Arias

et al. 2019

Stem Cells International

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Articular cartilage injuries remain as a therapeutic challenge due to the limited regeneration potential of this tissue. Cartilage engineering grafts combining chondrogenic cells, scaffold materials, and microenvironmental factors are emerging as promissory alternatives. The design of an adequate scaffold resembling the physicochemical features of natural cartilage and able to support chondrogenesis in the implants is a crucial topic to solve. This study reports the development of an implant constructed with IGF1-transduced adipose-derived mesenchymal stem cells (immunophenotypes: CD105+, CD90+, CD73+, CD14-, and CD34-) embedded in a scaffold composed of a mix of alginate/milled bovine decellularized knee material which was cultivated in vitro for 28 days (3CI). Histological analyses demonstrated the distribution into isogenous groups of chondrocytes surrounded by a de novo dense extracellular matrix with balanced proportions of collagens II and I and high amounts of sulfated proteoglycans which also evidenced adequate cell proliferation and differentiation. This graft also shoved mechanical properties resembling the natural knee cartilage. A modified Bern/O’Driscoll scale showed that the 3CI implants had a significantly higher score than the 2CI implants lacking cells transduced with IGF1 (16/18 vs. 14/18), representing high-quality engineering cartilage suitable for in vivo tests. This study suggests that this graft resembles several features of typical hyaline cartilage and will be promissory for preclinical studies for cartilage regeneration.

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“…In cartilage, permeability affects fluid pressurization in the tissue, hydration, and the loading imposed on collagen fibrils, 70 all of which may influence collagen crimp and/or recruitment. 71 – 74 During myopigenesis, aggrecan and sGAGs appear to be the most rapidly altered component in the sclera, 10 and other work shows evidence of collagen crimp being rapidly increased in the myopic sclera. 13 We hypothesize that loss of sGAGs in the sclera is causal to the increased tissue permeability, subsequently decreasing fluid pressurization and loading of the collagen fibrils, resulting in increased crimping of collagen fibrils that translates to a decrease in the tissue-level tensile modulus.…”

Section: Discussionmentioning

confidence: 92%

Altered Structure and Function of Murine Sclera in Form-Deprivation Myopia

Brown

Kowalski

Paulus

et al. 2022

Invest. Ophthalmol. Vis. Sci.

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Purpose The sclera is believed to biomechanically influence eye size, facilitating the excessive axial elongation that occurs during myopigenesis. Here, we test the hypothesis that the sclera will be remodeled and exhibit altered biomechanics in the mouse model of form-deprivation (FD) myopia, accompanied by altered retinoid concentrations, a potential signaling molecule involved in the process. Methods Male C57 Bl/6J mice were subjected to unilateral FD ( n = 44 eyes), leaving the contralateral eye untreated (contra; n = 44). Refractive error and ocular biometry were measured in vivo prior to and after 1 or 3 weeks of FD. Ex vivo measurements were made of scleral biomechanical properties (unconfined compression: n = 24), scleral sulfated glycosaminoglycan (sGAG) content (dimethylmethylene blue: n = 18, and immunohistochemistry: n = 22), and ocular all-trans retinoic acid (atRA) concentrations (retina and RPE + choroid + sclera, n = 24). Age-matched naïve controls were included for some outcomes ( n = 32 eyes). Results Significant myopia developed after 1 (−2.4 ± 1.1 diopters [D], P < 0.001) and 3 weeks of FD (−4.1 ± 0.7 D, P = 0.025; mean ± standard deviation). Scleral tensile stiffness and permeability were significantly altered during myopigenesis (stiffness = −31.4 ± 12.7%, P < 0.001, and permeability = 224.4 ± 205.5%, P < 0.001). Total scleral sGAG content was not measurably altered; however, immunohistochemistry indicated a sustained decrease in chondroitin-4-sulfate and a slower decline in dermatan sulfate. The atRA increased in the retinas of eyes form-deprived for 1 week. Conclusions We report that biomechanics and GAG content of the mouse sclera are altered during myopigenesis. All scleral outcomes generally follow the trends found in other species and support a retina-to-sclera signaling cascade underlying mouse myopigenesis.

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Proteoglycans contribute locally to swelling, but globally to compressive mechanics, in intact cervine medial meniscus

Cited by 12 publications

References 23 publications

Phase-contrast enhanced synchrotron micro-tomography of human meniscus tissue

Phase-contrast enhanced synchrotron micro-tomography of human meniscus tissue

A Bioactive Cartilage Graft of IGF1-Transduced Adipose Mesenchymal Stem Cells Embedded in an Alginate/Bovine Cartilage Matrix Tridimensional Scaffold

Altered Structure and Function of Murine Sclera in Form-Deprivation Myopia

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