Proteoglycans and glycosaminoglycans induce gap junction synthesis and function in primary liver cultures.

Abstract

Abstract. Intercellular communication via gap junctions, as measured by dye and electrical coupling, disappears within 12 h in primary rat hepatocytes cultured in serum-supplemented media or within 24 h in cells in a serum-free, hormonaily defined medium (HDM) designed for hepatocytes. Glucagon and linoleic acid/BSA were the primary factors in the HDM responsible for the extended life span of the electrical coupling. After 24 h of culture, no hormone or growth factor tested could restore the expression of gap junctions. After 4-5 d of culture, the incidence of coupling was undetectable in a serum-supplemented medium and was only 4-5 % in HDM alone.

Gap Junction-Mediated Astrocytic Networks in the Mouse Barrel Cortex

“…Finally, the compartmentalized expression of Cxs could be attributable to a difference in the expression of extracellular matrix and/or adhesion molecules reported to occur in the barrel cortex, as for instance the perineuronal net (McRae et al, 2007) or cadherins (Gil et al, 2002). Indeed, such compartmentalized pattern could affect the expression of astrocytic Cxs and GJC because proteoglycans (Spray et al, 1987) and cadherins (Giepmans, 2004) have been reported to control Cx expression and function.…”

Cell biology of arterial proteoglycans.

“…123 Proteoglycans also appear to induce the formation of gap junctions in primary liver cultures by stimulating the synthesis of a specific gap junction protein. 124 It remains to be seen whether a similar situation exists for other types of cells such as vascular endothelial and smooth muscle cells.…”

Different changes in expression and function of connexin 26 and connexin 32 during DNA synthesis and redifferentiation in primary rat hepatocytes using a DMSO culture system

“…In condition 2, the extensive spreading of LY was observed in some of the cells teins Cx26 and Cx32 are very difficult. In primary cultures, Spray et al 4 showed that extracellular matrix components, from 72 to 96 hours, and the dye spread at 120 hours showed 75% recovery of the pretreatment control level (Fig. 8D and such as proteoglycans and glycosaminoglycans, can induce the synthesis and expression of Cx32 and that dibutyl cyclic E and Fig.…”

“…In the present cultures, almost confluent hepatocytes cultured in the medium Gap junctions are intercellular membrane channels that containing EGF with 2% DMSO and 10 07 mol/L glucagon, link neighboring cells and mediate reciprocal exchanges of underwent a nearly synchronous wave of DNA synthesis insmall molecules of less than 1,000 Da and ions, including duced by the removal of 2% DMSO and 10 07 mol/L glucagon, second messengers, such as cyclic adenosine monophosphate and the addition of 10 mmol/L nicotinamide, after which the inositol triphosphate, and Ca 2/ , between adjacent cells in DNA synthesis was completely re-inhibited by the re-addition contact. [1][2][3][4] Gap junctions are composed of proteins termed of 2% DMSO and 10 07 mol/L glucagon. During stimulation of ''connexins (Cxs)''.…”