Proteoglycan isolated from Phellinus linteus activates murine B lymphocytes via protein kinase C and protein tyrosine kinase

Kim, Gi‐Young; Park, Soonkew; Lee, Min-Ki; Lee, Sang-Hwa; Oh, Yang-Hyo; Kwak, Jong‐Young; Yoon, Sik; Lee, Jae-Dong; Park, Yeong‐Min

doi:10.1016/s1567-5769(03)00115-2

Cited by 55 publications

(32 citation statements)

References 18 publications

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“…7,[11][12][13] Since PPC has been reported to exhibit various immunomodulating activities, we purified novel PPC and focused on its activation of lymphocytes and macrophages, investigating its capacity for the proliferation of lymphocytes, to augment the production of cytokines and NO by macrophages and to increase the cytotoxicity of NK cells. Our results demonstrate that PPC had typical immunostimulatory activity, in that it selectively activated B cells, macrophages and NK cells, but not T cells in vitro.…”

Section: Discussionmentioning

confidence: 99%

“…After 100 mg/ml of PPC stimulation, the B cell proliferation was found to be 8 times higher than that of unstimulated B cells. 11,12) In particular, PPC like LPS was found to A, PPC was added at concentrations from 0 to 100 mg/ml to whole splenocytes. B, PPC at concentrations from 0 to 100 mg/ml was added to fractionate B or T cells.…”

Section: Induction Of the Proliferation Of Murine Splenocytes By Ppcmentioning

confidence: 99%

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Partial Characterization and Immunostimulatory Effect of a Novel Polysaccharide–Protein Complex Extracted fromPhellinus linteus

Kim

Lee

et al. 2006

Bioscience, Biotechnology, and Biochemistry

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Section: Discussionmentioning

confidence: 99%

Section: Induction Of the Proliferation Of Murine Splenocytes By Ppcmentioning

confidence: 99%

Partial Characterization and Immunostimulatory Effect of a Novel Polysaccharide–Protein Complex Extracted fromPhellinus linteus

Kim

Lee

et al. 2006

Bioscience, Biotechnology, and Biochemistry

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“…It has been reported that extracts derived from certain mushrooms can elicit an innate immune response, resulting in activation of NF-kB, and strongly stimulate cellular and humoral immunity [Kim et al, 2003;Kuo et al, 2006]. These mushroom extracts induce phenotypic and functional maturation of dendritic cells, tumoricidal activity in macrophages, and augmentation of natural killer cell activity [Sorimachi et al, 2001;Kodama et al, 2005;Kim et al, 2006].…”

Section: Introductionmentioning

confidence: 99%

Induction of cross‐protective immunity against influenza A virus H5N1 by an intranasal vaccine with extracts of mushroom mycelia

Ichinohe

Ainai

Nakamura

et al. 2009

Journal of Medical Virology

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The identification of a safe and effective adjuvant that is able to enhance mucosal immune responses is necessary for the development of an efficient inactivated intranasal influenza vaccine. The present study demonstrated the effectiveness of extracts of mycelia derived from edible mushrooms as adjuvants for intranasal influenza vaccine. The adjuvant effect of extracts of mycelia was examined by intranasal co-administration of the extracts and inactivated A/PR8 (H1N1) influenza virus hemagglutinin (HA) vaccine in BALB/c mice. The inactivated vaccine in combination with mycelial extracts induced a high anti-A/PR8 HA-specific IgA and IgG response in nasal washings and serum, respectively. Virus-specific cytotoxic T-lymphocyte responses were also induced by administration of the vaccine with extract of mycelia, resulting in protection against lethal lung infection with influenza virus A/PR8. In addition, intranasal administration of NIBRG14 vaccine derived from the influenza A/Vietnam/1194/2004 (H5N1) virus strain administered in conjunction with mycelial extracts from Phellinus linteus conferred cross-protection against heterologous influenza A/Indonesia/6/2005 virus challenge in the nasal infection model. In addition, mycelial extracts induced proinflammatory cytokines and CD40 expression in bone marrow-derived dendritic cells. These results suggest that mycelial extract-adjuvanted vaccines can confer cross-protection against variant H5N1 influenza viruses. The use of extracts of mycelia derived from edible mushrooms is proposed as a new safe and effective mucosal adjuvant for use for nasal vaccination against influenza virus infection.

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“…These complex polysaccharides have been detected in a variety of different mushroom species and linked to the immunostimulatory and antitumour activities (Wasser, 2002). Therefore, PL stimulated proliferation of T lymphocytes and activated B cells (Kim et al, 1996(Kim et al, , 2003c, and induced maturation of bone marrow-derived dendritic cells and the macrophage response (Kim et al, 2003a). On the other hand, PL demonstrated antiinflammatory effects in lipopolysaccharide-stimulated macrophages (Kim et al, 2006).…”

mentioning

confidence: 99%

Phellinus linteus suppresses growth, angiogenesis and invasive behaviour of breast cancer cells through the inhibition of AKT signalling

et al. 2008

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The antitumour activity of a medicinal mushroom Phellinus linteus (PL), through the stimulation of immune system or the induction of apoptosis, has been recently described. However, the molecular mechanisms responsible for the inhibition of invasive behaviour of cancer cells remain to be addressed. In the present study, we demonstrate that PL inhibits proliferation (anchorage-dependent growth) as well as colony formation (anchorage-independent growth) of highly invasive human breast cancer cells. The growth inhibition of MDA-MB-231 cells is mediated by the cell cycle arrest at S phase through the upregulation of p27Kip1 expression. Phellinus linteus also suppressed invasive behaviour of MDA-MB-231 cells by the inhibition of cell adhesion, cell migration and cell invasion through the suppression of secretion of urokinase-plasminogen activator from breast cancer cells. In addition, PL markedly inhibited the early event in angiogenesis, capillary morphogenesis of the human aortic endothelial cells, through the downregulation of secretion of vascular endothelial growth factor from MDA-MB-231 cells. These effects are mediated by the inhibition of serinethreonine kinase AKT signalling, because PL suppressed phosphorylation of AKT at Thr 308 and Ser 473 in breast cancer cells. Taken together, our study suggests potential therapeutic effect of PL against invasive breast cancer.

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Proteoglycan isolated from Phellinus linteus activates murine B lymphocytes via protein kinase C and protein tyrosine kinase

Cited by 55 publications

References 18 publications

Partial Characterization and Immunostimulatory Effect of a Novel Polysaccharide–Protein Complex Extracted fromPhellinus linteus

Partial Characterization and Immunostimulatory Effect of a Novel Polysaccharide–Protein Complex Extracted fromPhellinus linteus

Induction of cross‐protective immunity against influenza A virus H5N1 by an intranasal vaccine with extracts of mushroom mycelia

Phellinus linteus suppresses growth, angiogenesis and invasive behaviour of breast cancer cells through the inhibition of AKT signalling

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