Proteoglycan fragments in joint fluid

Dahlberg, Leif; Ryd, Leif; Lohmander, L. Stefan

doi:10.3109/17453679209154758

Cited by 78 publications

(11 citation statements)

References 37 publications

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“…Although both the quality and the quantity of the joint cartilage are suggested to influence the SF levels of biomarkers [19], we found no correlation between SF ARGS and radiographic status at the time of sampling in this dataset. Inclusion of the JSN sum score at examination A (our best approximation of joint-cartilage quantity) as an adjustment in the logistic regression model, did not essentially change the results (data not shown).…”

Section: Discussioncontrasting

confidence: 64%

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Association between synovial fluid levels of aggrecan ARGS fragments and radiographic progression in knee osteoarthritis

et al. 2010

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IntroductionAggrecanase cleavage at the 392Glu-393Ala bond in the interglobular domain (IGD) of aggrecan, releasing N-terminal 393ARGS fragments, is an early key event in arthritis and joint injuries. We determined whether synovial fluid (SF) levels of ARGS-aggrecan distinguish subjects with progressive radiographic knee osteoarthritis (ROA) from those with stable or no ROA.MethodsWe studied 141 subjects who, at examination A, had been given meniscectomies an average of 18 years earlier (range, 15 to 22 years). Seventeen individuals without surgery, and without known injury to the menisci or cruciate ligaments, were used as references. At examinations A and B, with a mean follow-up time of 7.5 years, we obtained SF and standing tibiofemoral and skyline patellofemoral radiographs. SF ARGS-aggrecan was measured with an electrochemiluminescence immunoassay, and we graded radiographs according to the OARSI atlas. The association between SF ARGS levels at examination A and progression of radiographic features of knee OA between examinations A and B was assessed by using logistic regression adjusted for age, gender, body mass index, and time between examinations, and stratified by ROA status at examination A.ResultsWe found a weak negative association between SF ARGS concentrations and loss of joint space: the likelihood of progression of radiographic joint space narrowing decreased 0.9 times per picomole per milliliter increase in ARGS (odds ratio (OR) 0.89; 95% confidence interval (CI), 0.79 to 0.996). In subjects with and without preexisting ROA at examination A, the association was OR, 0.96; 0.81 to 1.13; and 0.77; 0.62 to 0.95, respectively. Average levels of SF ARGS 18 years after meniscectomy were no different from those of reference subjects and were not correlated to radiographic status at examination A.ConclusionsIn subjects with previous knee meniscectomy but without ROA, levels of SF ARGS-aggrecan were weakly and inversely associated with increased loss of joint space over a period of 7.5 years.

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Section: Discussioncontrasting

confidence: 64%

“…Multiple reports have been published on aggrecan release into SF in disease [18,19,21,38,41-43]. After knee injury, SF levels of aggrecan were initially much elevated, but with time declined toward levels seen in uninjured knees [18,38].…”

Section: Discussionmentioning

confidence: 99%

Association between synovial fluid levels of aggrecan ARGS fragments and radiographic progression in knee osteoarthritis

et al. 2010

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“…In an inflamed joint of a patient with RA, hyperplasia of stromal cells and infiltration of inflammatory cells into the synovium causes localized pathophysiological changes including lower oxygenation (hypoxia), 13 increased blood vessel formation (synovial angiogenesis), 14 and an increase in leukocyte and protein concentration, 15 consequently altering the optical properties compared to healthy joints. A nontomographic, single wavelength, CW transillumination device was previously presented for detecting these optical changes, in which a light source was focused on the dorsal side of the finger, with images collected using a CCD camera on the palmar side.…”

Section: Introductionmentioning

confidence: 99%

Detecting inflammation in rheumatoid arthritis using Fourier transform analysis of dorsal optical transmission images from a pilot study

2019

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A clinical need exists for low-cost and noninvasive imaging tools capable of detecting inflammation in the joints of inflammatory arthritis patients. Previous studies have reported an optical contrast between inflamed and noninflamed joints resulting from distinct absorption and scattering properties. Accurate classification using nonocclusion-based continuous wave, transillumination imaging was limited to patient-specific changes during follow-up examination as opposed to single time-point examination, which was attributed to high intersubject variability. In distinction from previous work, optical images were acquired from the dorsal side with illumination on the palmar side and features about the spatial distribution of transmitted light along the joint were assessed using a normalized Fourier transform method. Results using this approach demonstrated an area under receiver operator curve of up to 0.888 for detecting inflammation in a pilot study involving single time-point examination of 144 joints from 21 rheumatology patients. This workflow may enable future development of clinically viable, lowcost devices for assessing inflammation in arthritis patients, without the need for cuff occlusion or comparison to baseline. © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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“…Additionally, it has been found that the PG produced by the chondrocytes in osteoarthritic cartilage is similar to juvenile PG with respect to both the amount of chondroitin‐6‐sulfate and chondroitin‐4‐sulfate, and to the amount of keratan sulfate produced (15). Furthermore, PG fragments can be found in synovial fluid (16).…”

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confidence: 99%

²³Na MRI accurately measures fixed charge density in articular cartilage

Shapiro

Borthakur

Gougoutas

et al. 2002

Magnetic Resonance in Med

260

208

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One of the initiating steps of osteoarthritis is the loss of proteoglycan (PG) molecules from the cartilage matrix. One method for assessing cartilage integrity, therefore, is to measure the PG content or fixed charge density (FCD) of cartilage. This report shows the feasibility of calculating FCD by 23 Na MRI and introduces MRI protocols for human studies, in vivo.23 Na MRI was used to measure the sodium concentration inside bovine patellar cartilage. The sodium concentration was then converted to FCD (mM) by considering ideal Donnan equilibrium. These FCD measurements were compared to FCD measurements obtained through standard dimethylmethylene blue PG assays. There was a high correlation (slope ‫؍‬ 0.89, r 2 ‫؍‬ 0.81) between the FCD measurements obtained by 23 Na MRI and those obtained by the PG assays. These methods were then employed in quantifying the FCD of articular cartilage of human volunteers in vivo. Two imaging protocols were compared: one using a birdcage coil, the other using a transmit/receive surface coil. Both methodologies gave similar results, with the average sodium concentration of normal human patellar cartilage ranging from ϳ ϳ240 to 260 mM. This corresponds to

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Proteoglycan fragments in joint fluid

Cited by 78 publications

References 37 publications

Association between synovial fluid levels of aggrecan ARGS fragments and radiographic progression in knee osteoarthritis

Association between synovial fluid levels of aggrecan ARGS fragments and radiographic progression in knee osteoarthritis

Detecting inflammation in rheumatoid arthritis using Fourier transform analysis of dorsal optical transmission images from a pilot study

²³Na MRI accurately measures fixed charge density in articular cartilage

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Proteoglycan fragments in joint fluid

Cited by 78 publications

References 37 publications

Association between synovial fluid levels of aggrecan ARGS fragments and radiographic progression in knee osteoarthritis

Association between synovial fluid levels of aggrecan ARGS fragments and radiographic progression in knee osteoarthritis

Detecting inflammation in rheumatoid arthritis using Fourier transform analysis of dorsal optical transmission images from a pilot study

23Na MRI accurately measures fixed charge density in articular cartilage

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²³Na MRI accurately measures fixed charge density in articular cartilage