ProteoChip-based library screening of integrin α5β1 antagonists from korean medicinal plant extracts

Bang, Ji Young; Kim, Eung-Yoon; Seong, Nak Sul; Shin, Yoo Soo; Kang, In‐Cheol

doi:10.1007/bf02977328

Cited by 7 publications

(3 citation statements)

References 23 publications

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“…A5-1, a peptide selected through proteoChip-based library screening, is effective as an angiogenistic inhibitior against integrin α5β1 10 . Korean medicinal plant extracts chosen under the same methodology have been verified for their anti-angiogenic functions 11 .…”

Section: Introductionmentioning

confidence: 99%

Analysis of anti-angiogenic mechanism of HangAmDan-B (HAD-B), a Korean traditional medicine, using antibody microarray chip

et al. 2010

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The inhibitory effects of the water extract of HangAmDan-B (WEHAD-B), which is a crude extract of eight Korean medicinal animals and plants on bFGF-induced neovascularization were investigated. WEHAD-B significantly prevented bFGF-induced HUVE cell proliferation, adhesion, migration, and capillary-like tubular network formation. Half-maximal inhibition of proliferation on the endothelial cells by WEHAD-B was observed at a concentration of approximately 250 μg/mL. Our antibody microarray-based ProteoChip data showed that WEHAD-B increased the expression of STAT1 and Rb2, which are involved in cell growth, apoptosis, and controlling the cell cycle in bFGF-induced HUVECs. These results indicate that the inhibition of bFGF-induced angiogenesis by WEHAD-B may be due to upregulation of cell signaling proteins, STAT1 and Rb2. The blood vessel formation in a chick chorioallantoic membrane (CAM) treated with WEHAD-B was markedly reduced in length compared with a PBS-treated control group. Taken together, these data suggest that antibody-arrayed ProteoChip technology may be an useful tool for determining molecular mechanism of natural products in biological samples.

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Section: Introductionmentioning

confidence: 99%

Analysis of anti-angiogenic mechanism of HangAmDan-B (HAD-B), a Korean traditional medicine, using antibody microarray chip

et al. 2010

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“…Protein microarray technology has recently led to a wide range of biological applications, which range from proteo-mic research in microbes to mammals with high stringency of identification [34][35][36][37]. One possible application of highdensity antibody microarray for global analysis of protein expression profiles was also demonstrated.…”

Section: Discussionmentioning

confidence: 99%

Protein chip analysis of pluripotency‐associated proteins in NIH3T3 fibroblast

Ajjappala

Kim

et al. 2009

Proteomics

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Specific transcription factors regulate the totipotent and pluripotent capability of embryonic stem cells. Amongst these regulatory transcription factors in embryonic stem cells, Oct4 and Nanog are master factors that also have unique characteristic ability of cell-specific pluripotency and self-renewal. The expression of Nanog in fibroblasts confirms increased cell proliferation and transformation of foci-forming phenotype indicative of its oncogenic potential. The expression of Oct4, interestingly, leads to transformation of non-tumorgenic mouse into tumorigenic mouse. Our current investigation ascertains that the resultant increase in DNA synthesis and cell proliferation is the consequence of transforming the phenotype into foci formation. We used a manually curetted ProteoChip to carry out the signaling protein microarray analysis, which revealed up-regulated expression of various proteins including FAK1, MEK1 and Raf1. Some of the proteins explain the mechanism by which Oct4 and Nanog transform the phenotype. In NIH3T3 cells expressed with mouse Oct4 (mOct4), mouse Nanog (mNanog) separately as well as together, the specific knockdown of mFAK1 inhibited morphological transformation of the cells, and their invasion activity. The mFAK1 overexpression leads to morphological transformation as shown with mOct4 and mNanog. Additionally, we showed that the ERK1/2 pathway is involved in the up-regulation of c-myc and cyclin D1 expression mediated by mFAK1. Our results signify that the combinatorial signaling protein-array using biomolecular approach may possibly provide us with a new tool to understand cellular homeostasis.

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“…Therapeutic inhibitors have been found using ProteoChip-based library screening methods. For instance, the A5-1 peptide that is effective as an angiogenesis inhibitor against integrin α5β1 [10], and certain Korean medicinal plant extracts chosen under the same methodology have been verified to possess anti-angiogenic functions [11], have been identified using this methodology.…”

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confidence: 99%

Anti-angiogenic effects of the water extract of HangAmDan (WEHAD), a Korean traditional medicine

Bang

Kim

et al. 2011

Sci. China Life Sci.

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We investigated the anti-angiogenic effects of the water extract of HangAmDan (WEHAD), which is a crude extract of nine Korean medicinal substances of animal and plant origin. In human umbilical vein endothelial cells, WEHAD significantly inhibited bFGF-induced proliferation, adhesion, migration, and capillary tube formation. We used an antibody array to perform an analysis of signaling proteins, which showed up-regulated expression of various proteins including RAD51, RAD52, and p73, and down-regulated expression of pFAK. Blood vessel formation in a chick chorioallantoic membrane (CAM) treated with WEHAD was markedly reduced in length compared with a PBS-treated control group. These results suggest that inhibition of angiogenesis by WEHAD may be the mechanism of action for the anti-cancer effects of HAD.angiogenesis, HangAmDan, WEHAD, antibody array, HUVEC Citation:

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ProteoChip-based library screening of integrin α5β1 antagonists from korean medicinal plant extracts

Cited by 7 publications

References 23 publications

Analysis of anti-angiogenic mechanism of HangAmDan-B (HAD-B), a Korean traditional medicine, using antibody microarray chip

Analysis of anti-angiogenic mechanism of HangAmDan-B (HAD-B), a Korean traditional medicine, using antibody microarray chip

Protein chip analysis of pluripotency‐associated proteins in NIH3T3 fibroblast

Anti-angiogenic effects of the water extract of HangAmDan (WEHAD), a Korean traditional medicine

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