Proteins of rat serum, urine, and cerebrospinal fluid: VI. Further protein identifications and interstrain comparison

Abstract

We have investigated the biological fluids--serum, cerebrospinal fluid, and urine--of three strains of rats; the present data extend our database (also available on-line) and may be of interest for pharmacological and toxicological investigation. Specifically, we have defined reference maps of the major protein components in cerebrospinal fluid and urine. Compartment-specific isoforms were recognized for transferrin and transthyretin. Mass spectrometric data established the cleavage site of the signal peptide and identified the N-terminal blocking group of prostaglandin D synthase from rat cerebrospinal fluid. A previously undescribed member of the family of low molecular mass rat urinary proteins was characterized as containing a sequence similar, but not identical, to the N-terminal region of rat urinary protein-2 (RUP-2), and divergent from RUP-1.

Chemical Characterization of Territorial Marking Fluid of Male Bengal Tiger, Panthera tigris

“…These small polypeptide subunits are components of immunoglobulins, T cell receptors, CD1 cell surface glycoproteins, secretory glycoproteins, and major histocompatibility complex class I/II molecules and have previously been identified in the urine of rodents (Hurst et al 2005). Transthyretin is known to act as a carrier protein for various molecules, including retinol (Monaco 2000), and has been identified in rat urine (Wait et al 2001). Healthy mammals excrete small amounts of urinary proteins, and usually proteinuria is an indicator of renal abnormality (Raila et al 2005).…”

“…The solvent was blown off in a slow stream of nitrogen, then the residue was dissolved in water (50 μl) and acidified (pH 1) with H2SO4 (1.5 M). The organic material was extracted twice with dichloromethane (50 μl) and the extract was washed with water, dried over anhydrous magnesium sulfate, and analyzed by chiral GC and GC-MS using capillary column C. The product, 2- Dimethyl succinate (33), dimethyl glutarate (43), and dimethyl adipate (55) were synthesized by acid-catalyzed esterification of the corresponding dicarboxylic acids with methanol (Furniss, 1989). The mass spectra of the products were in agreement with mass spectral data in the NBS and NIST mass …”

“…The syntheses of 3-methyl-2-octanone (10), 3-methyl-2-nonanone (14), (R)-2-methylheptanoic acid, 2-methylnonanoic acid (77), 2-methyldecanoic acid (84), 2-methylundecanoic acid (89), dimethyl succinate (33), dimethyl glutarate (43), dimethyl adipate (55), N-benzylidenepentylamine (51), N-pentylurea (104), N-benzylidene-2-phenylethylamine (117), and (S)-(+)-(Z)-6-dodecen-4-olide (86) are described in the Supplementary Information for this article.…”

Proteomic Analysis of Articular Cartilage Shows Increased Type II Collagen Synthesis in Osteoarthritis and Expression of Inhibin βA (Activin A), a Regulatory Molecule for Chondrocytes

“…In-gel trypsinolysis was performed using an Investigator Progest (Genomic Solutions, Huntingdon, UK) robotic digestion system, as described previously (13). Tandem electrospray mass spectra were recorded using a Q-Tof hybrid quadrupole/orthogonal acceleration timeof-flight spectrometer (Waters, Manchester, UK) interfaced to a Waters CapLC capillary chromatograph.…”

Identification of Selective Basophil Chemoattractants in Human Nasal Polyps as Insulin-Like Growth Factor-1 and Insulin-Like Growth Factor-2

“…In-gel trypsinolysis was performed using an Investigator Progest (Genomic Solutions, Huntingdon, U.K.) robotic digestion system, as previously described (27). The resulting mixtures of peptides were characterized by tandem electrospray HPLC mass spectrometry on a Micromass Q-Tof spectrometer interfaced to a Waters CapLC chromatograph (28).…”