Proteins of rat serum: III. Gender‐related differences in protein concentration under baseline conditions and upon experimental inflammation as evaluated by two‐dimensional electrophoresis

Miller, Ingrid; Haynes, Paul A.; Eberini, Ivano; Gemeiner, Manfred; Aebersold, Ruedi; Gianazza, Elisabetta

doi:10.1002/9783527613489.ch33

From Genome to Proteome 1999

DOI: 10.1002/9783527613489.ch33

|View full text |Cite

Proteins of rat serum: III. Gender‐related differences in protein concentration under baseline conditions and upon experimental inflammation as evaluated by two‐dimensional electrophoresis

Ingrid Miller

Paul A. Haynes

Ivano Eberini

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2010

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

(1 citation statement)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Extracted peptides were analyzed using nanoLC‐MS/MS with a LTQ‐XL linear ion trap mass spectrometer (Thermo, CA, USA). Mass spectrometric data acquisition and data analysis parameters were essentially as described in 7–9, with MS/MS of the top six ions and the dynamic exclusion window set to 90 s as described in 10. Raw data files were converted to mzXML format and processed through the global proteome machine software using version 2.1.1 of X!Tandem 11.…”

mentioning

confidence: 99%

Shotgun proteomic profiling of five species of New Zealand Pachycladon

et al. 2010

View full text Add to dashboard Cite

The genus Pachycladon consists of ten species of alpine plants, nine of which are endemic to New Zealand. The species are closely related to the model plant Arabidopsis thaliana with respect to their sequence divergence and chromosome synteny, occupy distinct geographical habitats in terms of both latitude and altitude, and display a range of morphologies. We have performed label‐free quantitative shotgun proteomic analysis of five different species of Pachycladon, namely P. cheesemanii (CH), P. exile (EX), P. fastigiatum (FA), P. enysii (EN) and P. novae‐zelandiae (NZ). The total non‐redundant data set for all five species contained 1489 proteins. The numbers of proteins identified reproducibly in each species ranged from 629 for CH to 987 for NZ, with 681 for EN, 741 for EX and 934 for FA. Previous metabolite‐based studies have shown that FA hydrolyzes glucosinolates completely to isothiocyanates while EN converts glucosinolates to nitriles. In this study, we observed high expression of ESP (At1g54040, epithiospecifying senescence regulator protein) and myrosinase 2 (At5g25980, glycosyl hydrolase family protein), which result in production of nitriles and epithionitriles, in EN and NZ, and we also observed higher expression of ESM1 (At3g14210, GDSL esterase/lipase), which mediates the formation of isothiocyanate, in FA.

show abstract

mentioning

confidence: 99%

Shotgun proteomic profiling of five species of New Zealand Pachycladon

et al. 2010

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Proteins of rat serum: III. Gender‐related differences in protein concentration under baseline conditions and upon experimental inflammation as evaluated by two‐dimensional electrophoresis

Cited by 1 publication

References 56 publications

Shotgun proteomic profiling of five species of New Zealand Pachycladon

Shotgun proteomic profiling of five species of New Zealand Pachycladon

Contact Info

Product

Resources

About