Proteins of <i>Thermus thermophilus</i> Are Resistant to Glycation‐Induced Protein Precipitation: An Evolutionary Adaptation to Life at Extreme Temperatures?

Münch, Gerald; Berbaum, Katrin; Urban, Christin; Schinzel, Reinhard

doi:10.1196/annals.1333.098

Cited by 3 publications

(2 citation statements)

References 26 publications

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“…Clearly, glycosylation may be one such way to modify the proteins, but other modifications also exist, some of which, such as acetylation (69) or nonenzymatic glycation (4,45), have been observed in eubacteria. Interestingly, glycated proteins arise when intermediates in sugar metabolism, such as methylglyoxal (67) or 6-phosphogluconolactone (4), accumulate, resulting in protein inactivation and toxic side effects such as protein precipitation (47), suggesting that a cellular mechanism should exist for the targeted degradation of such products.…”

Section: Discussionmentioning

confidence: 99%

Conserved Network of Proteins Essential for Bacterial Viability

et al. 2009

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Section: Discussionmentioning

confidence: 99%

Conserved Network of Proteins Essential for Bacterial Viability

et al. 2009

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“…protein to aggregate, to be less soluble and to precipitate [5,7,8] . In addition, oxidation of albumin, for example, will reduce drug-binding properties and antioxidant properties and may induce tissue factor activity in culture of human umbilical vein endothelial cells [9] .…”

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confidence: 99%

Heparin Cryoprecipitation Reduces Plasma Levels of Non-Traditional Risk Factors for Atherosclerosis in vitro

Meilin

Sela²,

Kristal³

2008

Blood Purif

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Aims: To show that heparin cryoprecipitation (HCP), an in vitro method of plasma purification, reduces the levels of in vivo modified proteins and non-traditional risk factors from plasma of atherosclerotic hemodialysis (HD) patients. Methods: HCP was applied to plasma obtained from HD patients and controls, forming a precipitate – cryogel. Levels of fibrinogen, albumin, CRP, TNF-α, IL-6, advanced oxidation protein products, carbonylated fibrinogen and carbonylated albumin were determined in plasma before and after applying HCP and in the cryogel. Results: Treatment of HD plasma with HCP, beyond the significant reduction of the increased levels of all the above-mentioned molecules, reduced fibrinogen, TNF-α, carbonylated fibrinogen and carbonylated albumin to control levels which were simultaneously found in the cryogel. Conclusions: HCP applied to plasma enables the simultaneous precipitation of modified molecules and circulating non-traditional risk factors for atherosclerosis. This study may serve as a base for the future development of a clinical purification technique.

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Maillard Reactions in Hyperthermophilic Archaea: Implications for Better Understanding of Non-Enzymatic Glycation in Biology

Szwergold¹

2013

Rejuvenation Research

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Maillard reactions are an unavoidable feature of life that appear to be damaging to cell and organisms. Consequently, all living systems must have ways to protect themselves against this process. As of 2012, several such defense mechanisms have been identified. They are all enzymatic and were found in mesophilic organisms. To date, no systematic study of Maillard reactions and the relevant defense mechanisms has been conducted in thermophiles (50°C-80°C) or hyperthermophiles (80°C-120°C). This is surprisingly because Maillard reactions become significantly faster and potent with increasing temperatures. This review examines this neglected issue in two well-defined sets of hyperthermophiles. My analysis suggests that hyperthermophiles cope with glycation stress by several mechanisms: • Absence of glycation-prone head groups (such as ethanoalamine) from hyperthermophilic phospholipids • Protection of reactive carbohydrates and labile metabolic intermediates by substrate channeling. • Conversion of excess reactive sugars such as glucose to non-reactive compounds including trehalose, di-myo-inositol-phosphate and mannosylglycerate. • Detoxification of methylglyoxal and other ketoaldehydes by conversion to inert products through a variety of reductases and dehydrogenases. • Scavenging of the remaining carbonyls by nucleophilic amines, including a variety of novel polyamines. Disruption of the Maillard process at its early stages, rather than repair of damage caused by it at later stages, appears to be the preferred strategy in the organisms examined. The most unique among these mechanisms appears to be a polyamine-based scavenging system. Undertaking research of the Maillard process in hyperthermophiles is important in its own right and is also likely to provide new insights for the control of these reactions in humans, especially in diseases such as diabetes mellitus.

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Proteins of Thermus thermophilus Are Resistant to Glycation‐Induced Protein Precipitation: An Evolutionary Adaptation to Life at Extreme Temperatures?

Cited by 3 publications

References 26 publications

Conserved Network of Proteins Essential for Bacterial Viability

Conserved Network of Proteins Essential for Bacterial Viability

Heparin Cryoprecipitation Reduces Plasma Levels of Non-Traditional Risk Factors for Atherosclerosis in vitro

Maillard Reactions in Hyperthermophilic Archaea: Implications for Better Understanding of Non-Enzymatic Glycation in Biology

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