Proteins of herpesvirus type 2: I. Virion, nonvirion, and antigenic polypeptides in infected cells

Strnad, Bruce C.; Aurelian, Laure

doi:10.1016/0042-6822(76)90475-x

Cited by 33 publications

(18 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S T~A D and AURELIAN showed that the 47 viral proteins found in HSV-2infected HEp-2 cells could be classified into at least four groups on the basis of the kinetics of their synthesis (19). W h e n the present observations are compared with their results, proteins in group 2 or 3 of their classification seem to be probable candidates for the t u b u l a r structures.…”

Section: Discussionsupporting

confidence: 53%

Effects of antimetabolites on the production of tubular structures in vero cells infected with herpes simplex virus type 2

Oda

Mori

Miyazono

et al. 1979

Archives of Virology

View full text Add to dashboard Cite

Treatment of Vero cells infected with herpes simplex virus type 2 with the proper concentrations of hydroxyurea (HU) reduced the production of infectious virus and markedly increased the accumulation of tubular structures in the nuclei when the drug was added within 6 hours after infection. Similar accumulation of tubular structures in the infected nuclei was also observed when infected cells were treated with phosphonoacetic acid at proper concentrations. The release of HU-treated, herpes simplex virus type 2-infected cells from the drug-induced blocking of synthesis of infectious virus resulted in the marked decrease of tubular structures coincidentally with the beginning of production of infectious virus. The data suggest the possibility that the disappearance of tubular structures may be related to the active production of infectious virus.

show abstract

Section: Discussionsupporting

confidence: 53%

Effects of antimetabolites on the production of tubular structures in vero cells infected with herpes simplex virus type 2

Oda

Mori

Miyazono

et al. 1979

Archives of Virology

View full text Add to dashboard Cite

show abstract

“…into virally coded proteins. Separation of these proteins by polyacrylamide gel electrophoresis has so far allowed the enumeration of 51 or 52 polypeptides of viral origin (17,21) which have been identified by their order of migration (11) or by their apparent molecular weights (17). The large number of polypeptides detected, the variability in molecular weight estimates determined by polyacrylamide gel electrophoresis un-der varied conditions as well as viral strain and host cell differences, the difficulty of detecting gene products synthesized in minor amounts, and the lack of knowledge of possible precursorproduct relationships greatly hamper the usefulness of this system for identifying and studying viral proteins.…”

Section: Discussionmentioning

confidence: 99%

“…Specific antibodies to herpes simplex virus proteins offer a means of identifying and characterizing the interrelations and functions of these molecules. Antibodies directed against thymidine kinase (molecular weights, 44,000 and 42,000) of HSV-1 and HSV-2 (22), ICP-4 (molecular weight, 175,000) of HSV-1 (4), a nucleocapsid protein (molecular weight, 40,000) of HSV-1 and HSV-2 (21), glycoproteins gD (molecular weight, 52,000 to 59,000) (3), gBgA, and gC (6) have been raised in laboratory animals by injection of purified proteins. More recently, the production of monoclonal antibodies to glycoproteins gC and gD of HSV-1 (16) and to the 40,000molecular-weight nucleocapsid protein of HSV-1 (23) has been reported.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Monoclonal antibodies to two glycoproteins of herpes simplex virus type 2

Balachandran¹,

Harnish²,

Killington³

et al. 1981

J Virol

View full text Add to dashboard Cite

Monoclonal antibodies to herpes simplex virus type 2 were found to precipitate different numbers of radiolabeled polypeptides from lysates of virus-infected cells. Antibodies directed against two viral glycoproteins were characterized. Antibodies from hybridoma 17aA2 precipitated a 60,000-molecular-weight polypeptide which chased into a 66,000and a 79,000-molecular-weight polypeptide. All three polypeptides labeled in the presence of [3H]glucosamine and had similar tryptic digest maps. The 60,000-molecular-weight polypeptide also chased into a 31,000-molecular-weight species which did not label with [3H]glucosamine. Antibodies from hybridoma 17,8C2 precipitated a 50,000-molecular-weight polypeptide which chased into a 56,000and an 80,000-molecular-weight polypeptide. These polypeptides also shared a similar tryptic digest map and labeled with [3H]glucosamine. Both monoclonal antibodies were herpes simplex virus type 2 specific. The viral proteins precipitated by 17aA2 antibodies had characteristics similar to those reported for glycoprotein E, whereas the proteins precipitated by 17,BC2 antibodies appeared to represent a glycoprotein not previously described. This glycoprotein should be tentatively designated glycoprotein F.

show abstract

“…Other direct studies have included only one strain of HSV-2 (9,13,23 These incomplete data have served as a stimulus toward studies of polypeptide differences among a large number of HSV-2 strains.…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of the virion polypeptides specified by Herpes simplex virus type 2 strains

Cassai

Luca

Manservigi

et al. 1980

Archives of Virology

View full text Add to dashboard Cite

This paper reports on the variability of structural polypeptides of 32 strains of Herpes simplex virus 2 (HSV-2) isolated from various locations in the United States and Italy. Most strains were passaged a limited number of times at low multiplicity outside the human host; a few strains were characterized by numerous passages at variable multiplicities in cell culture. The acrylamide gel electrophoresis of polypeptides from purified virions revealed very few differences. Considering these differences the HSV-2 strains analysed can be classified into 3 groups according to the variability of three polypeptides. The study of polypeptides in HSV-2 infected cells revealed more differences than those seen in the structural proteins. Comparisons of the structural proteins specified by HSV-2 and HSV-1 virions, revealed clear variant features of the electrophoretic profiles, especially when pH of main gel was 8.9.

show abstract

Proteins of herpesvirus type 2: I. Virion, nonvirion, and antigenic polypeptides in infected cells

Cited by 33 publications

References 17 publications

Effects of antimetabolites on the production of tubular structures in vero cells infected with herpes simplex virus type 2

Effects of antimetabolites on the production of tubular structures in vero cells infected with herpes simplex virus type 2

Monoclonal antibodies to two glycoproteins of herpes simplex virus type 2

Comparative analysis of the virion polypeptides specified by Herpes simplex virus type 2 strains

Contact Info

Product

Resources

About