Proteins occurring at, or near, the subunit interface of E. coli ribosomes

E. coli 30 S and 50 S ribosomal subparticle components in the localization region of the tRNA acceptor terminus

“…Thus, proteins S3 and S14 are included in the group of three proteins (t S2) according to Randall-Haselbauer and Kurland [ 17 ] stimulating the enzymatic and nonenzymatic aminoacyl-tRNA binding with the 30 Stemplate complex and, as a consequence, participating according to the authors, in formation of the 30 S subparticle A-site. According to the data of Stiiffler's group [18] protein S14 occurs at,'or near, the ribosomal subparticle interface. Of the five proteins binding directly to 16 S-RNA, protein S7 is the only one situated in the 3' half of 16 S-RNA [ 191.…”

The primary structure of protein S5 from the small subunit of the Escherichia coli ribosome

“…Furthermore, protein S5 is, together with proteins S2 and S9, implied in the association of ribosomal subunits and stimulates the EF-G-dependent GTPase activity [3]. On the other hand, specific antibodies to protein S5 inhibit neither subunit reassociation [4] nor EF-G-dependent GTP-hydrolysis [ 51.…”

The primary structure of protein L27 from the peptidyl‐tRNA binding site of Escherichia coli ribosomes

“…Thus, protein L27 can be inferred to be very close to the peptidyl-tRNA binding site (P-site). In addition, antibodies against protein L27 inhibit the formation of 70S ribosomes [4]. This effect can be explained by the location of L27 at the interacting surface of the two subunits.…”