Proteins iodinated by the Chloramine-T method appear to be degraded at an abnormally rapid rate after endocytosis

Opresko, Lee K.; Wiley, H. Steven; Wallace, Robin A.

doi:10.1073/pnas.77.3.1556

Cited by 49 publications

(19 citation statements)

References 34 publications

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“…6, B and C) and the absence of significant degradation of VTG during its incorporation by oocytes (18) suggest that the light platelets are not simply a degradative compartment entered by a small percentage of the total ligand. The reason(s) for the second increase in density of yolk platelets to 1.23 g/cc are not currently known, nor is the mechanism for the apparent loss in enzyme activity during this final transition.…”

Section: Identity Of the Light Lysosome Fractionmentioning

confidence: 99%

An unusual lysosome compartment involved in vitellogenin endocytosis by Xenopus oocytes.

Wall¹,

Meleka²

1985

The Journal of Cell Biology

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We have investigated the lysosomal compartment ofXenopus oocytes to determine the possible role of this organelle in the endocytic pathway of the yolk protein precursor, vitellogenin. Oocytes have lysosome-like organelles of unusual enzymatic composition at all stages of their development, and the amount of hydrolase activity increases steadily throughout oogenesis. These unusual lysosomes appear to be located primarily in a peripheral zone of oocyte cytoplasm. At least two distinct populations of lysosomal organelles can be identified after sucrose density gradient fractionation of vitellogenic oocytes. Most enzyme activity resides in a compartment of large size and high density that appears to be a subpopulation of yolk platelets that are less dense than most platelets within the cell. The appearance of this high density peak of lysosomal enzyme activity coincides with the time of onset of vitellogenin endocytosis during oocyte development. The data suggest that endocytic vesicles that contain vitellogenin fuse with modified lysosomes shortly after their internalization by the oocyte. Pulse-chase experiments with radiolabeled vitellogenin suggest that the ligand passes through the low density platelet compartment en route to the heavy platelets. The accumulation of yolk proteins apparently results from a failure of these molecules to undergo complete digestion after their entry into an unusual lysosomal compartment. The yolk platelets that these proteins finally enter for prolonged storage appear to be a postlysosomal organelle.

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Section: Identity Of the Light Lysosome Fractionmentioning

confidence: 99%

An unusual lysosome compartment involved in vitellogenin endocytosis by Xenopus oocytes.

Wall¹,

Meleka²

1985

The Journal of Cell Biology

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“…But iodine radioisotope, which was labeled by the above method, was normally found to be unstable in living subjects and such deiodination resulted in high background or false signals. [14][15][16] Moreover direct radiolabeling using a strong oxidant for the formation of reactive iodo species often cause decreased biological activity of the substrate. Therefore several prosthetic groups have been developed for indirect labeling of iodine radioisotopes.…”

Section: Introductionmentioning

confidence: 99%

Efficient method for iodine radioisotope labeling of cyclooctyne-containing molecules using strain-promoted copper-free click reaction

Jeon

Kang

Shim

et al. 2015

Bioorganic & Medicinal Chemistry

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“…24 Radioactive counting was done with a γ-ray spectrometer after separating the melatonin from the reaction mixtures. 125 I]-Iodomelatonin administration, the rats were sacrificed through cervical dislocation and the amount of radioactivity in each tissue was determined with a γ-ray spectrometer and expressed as % uptake of total amount of injected dose per g of tissue.…”

Section: Iodinated Melatoninmentioning

confidence: 99%

Vesicular melatonin efficiently downregulates sodium fluoride-induced rat hepato- and broncho- TNF-α, TGF-β expressions, and associated oxidative injury: a comparative study of liposomal and nanoencapsulated forms

Sana

Ghosh

Das

et al. 2017

IJN

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The importance of fluoride as a natural and industrial toxicant is recognized worldwide. We evaluated the regulating role and biological effect of vesicular (liposomal and nanoencapsulated) melatonin (N-acetyl-5-methoxytryptamine) for drug delivery and controlled release on the depletion of inflammatory mediators, as well as oxidative damage in sodium fluoride (NaF)-treated lungs and liver. Hepatic and bronchial damage was induced in Swiss albino rats with a single acute ingestion of NaF (48 mg/kg body weight, oral gavage). NaF exposure caused the generation of reactive oxygen species (ROS); upregulation of TNF-α and TGF-β; decreased activities of antioxidant systems (glutathione, glutathione-S-transferase, superoxide dismutase, catalase), succinate dehydrogenase, membrane microviscosity, and membrane potential; increased activity of lipid peroxidation and nicotinamide adenine dinucleotide hydride oxidase; and increased hepatic and nephrite toxicities ( P <0.001) compared to those in normal animals. Charge (−ve/+ve)-specific single liposomal (dicetyl phosphate/stearylamine) and nanoencapsulated melatonin (4.46 mg/kg body weight, intravenous) treatments (2 hours after NaF exposure) significantly ( P <0.01/0.001) and maximally ( P <0.001) inhibited all alterations developed in NaF-mediated oxidative injuries in rat liver (+ve) and lungs (−ve), demonstrating their strong free radical scavenging, antioxidant and antigenotoxic properties, and vesicular efficiencies of targeting. Overall, these results suggest that nanoencapsulated melatonin might be considered as a more powerful remedial therapy in comparison to liposomes, in terms of its efficacy in regulating NaF-intoxicated oxidative injury.

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Proteins iodinated by the Chloramine-T method appear to be degraded at an abnormally rapid rate after endocytosis

Cited by 49 publications

References 34 publications

An unusual lysosome compartment involved in vitellogenin endocytosis by Xenopus oocytes.

An unusual lysosome compartment involved in vitellogenin endocytosis by Xenopus oocytes.

Efficient method for iodine radioisotope labeling of cyclooctyne-containing molecules using strain-promoted copper-free click reaction

Vesicular melatonin efficiently downregulates sodium fluoride-induced rat hepato- and broncho- TNF-α, TGF-β expressions, and associated oxidative injury: a comparative study of liposomal and nanoencapsulated forms

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Proteins iodinated by the Chloramine-T method appear to be degraded at an abnormally rapid rate after endocytosis

Cited by 49 publications

References 34 publications

An unusual lysosome compartment involved in vitellogenin endocytosis by Xenopus oocytes.

An unusual lysosome compartment involved in vitellogenin endocytosis by Xenopus oocytes.

Efficient method for iodine radioisotope labeling of cyclooctyne-containing molecules using strain-promoted copper-free click reaction

Vesicular melatonin efficiently downregulates sodium fluoride-induced rat hepato- and broncho- TNF-&alpha;, TGF-&beta; expressions, and associated oxidative injury: a comparative study of liposomal and nanoencapsulated forms

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Vesicular melatonin efficiently downregulates sodium fluoride-induced rat hepato- and broncho- TNF-α, TGF-β expressions, and associated oxidative injury: a comparative study of liposomal and nanoencapsulated forms