Proteins involved in the antioxidant and inflammatory response in rutin-treated human skin fibroblasts exposed to UVA or UVB irradiation

Gęgotek, Agnieszka; Domingues, Pedro; Skrzydlewska, Elżbieta

doi:10.1016/j.jdermsci.2018.02.002

Cited by 35 publications

(28 citation statements)

References 64 publications

(70 reference statements)

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“…Also, the protein-folding processes coordinated by heat shock proteins 70 and 90 (HSP70; HSP90) are protected from UV-induced disturbances by ascorbic acid and rutin. UV radiation, causing disruption in protein structures, leads to an increase in protein chaperone (HSP70, HSP32, HSP90) level and activity [36,49]. HSP70 interacts with all proteins in their unfolded, misfolded, or aggregated states, ensuring proper conformation and activity [50].…”

Section: Discussionmentioning

confidence: 99%

“…Only proteins with at least two unique peptides identified were taken for further analysis. The formation of rutin or lipid peroxidation products adducts with protein was detected as changes in the mass of the individual amino acid residues as follows: rutin-cysteine mass increase by 610.153, MDA-lysine mass increase by 72.021, 4-ONE-cysteine mass increase by 154.206, and 4-HNE-cysteine/lysine/histidine mass increase by 156.115 [35,36]. The number of peptides containing each modification was considered as the amount of each type of adducts and was presented in relation to the values found in the control cells.…”

Section: Protein Identification and Label-free Quantificationmentioning

confidence: 99%

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Cytoprotective Effect of Ascorbic Acid and Rutin against Oxidative Changes in the Proteome of Skin Fibroblasts Cultured in a Three-Dimensional System

Gęgotek

Jarocka-Karpowicz

2020

Nutrients

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The combination of ascorbic acid and rutin, commonly used in oral preparations for their antioxidant and anti-inflammatory properties, can also be used to protect skin cells from the effects of UV radiation in sunlight. Here, we tested the potential protective effect of ascorbic acid and rutin used together in UVB-irradiated human skin fibroblasts, and assessed the proteomic profile of these cells, grown in a three-dimensional (3D) system. Proteomic findings revealed a combined effect of ascorbic acid and rutin in UV-irradiated fibroblasts against overexpression of pro-inflammatory signaling proteins and DNA reorganization/expression. These effects were not observed when cells were treated with either compounds alone. The antioxidant effects of ascorbic acid and rutin also prevented protein modifications by lipid peroxidation products. Further, ascorbic acid stimulated rutin-protein adduct formation, which supports intra/extracellular signaling and the Nrf2/ARE antioxidant pathway, contributing to the protective effects against UV-induced oxidative stress. The combined effect of ascorbic acid and rutin suggests that this combination of compounds is potentially effective against skin damage caused by UV radiation.

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Section: Discussionmentioning

confidence: 99%

Section: Protein Identification and Label-free Quantificationmentioning

confidence: 99%

Cytoprotective Effect of Ascorbic Acid and Rutin against Oxidative Changes in the Proteome of Skin Fibroblasts Cultured in a Three-Dimensional System

Gęgotek

Jarocka-Karpowicz

2020

Nutrients

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“…Ascorbic acid and rutin cause also different changes in Keap1 levels. Decrease in Keap1 levels following rutin treatment might be connected with rutin-Keap1 adduct formation, which has been found in UV-irradiated skin fibroblasts [23]. Keap1 adduct formation changes the conformation of Keap1, leading to the Keap1-Nrf2 dissociation, as well as leading to Keap1 ubiquitination and degradation [37].…”

Section: Antioxidant Capacitymentioning

confidence: 94%

Rutin and ascorbic acid cooperation in antioxidant and antiapoptotic effect on human skin keratinocytes and fibroblasts exposed to UVA and UVB radiation

et al. 2019

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The combination of ascorbic acid and rutin is frequently used in oral preparations. However, despite numerous protective effects of each component individually, their combined effect on ultraviolet (UV)-irradiated skin cells has never been evaluated. The aim of this study was to evaluate the combined effect of ascorbic acid and rutin on human keratinocytes and fibroblasts exposed to UVA and UVB radiation. Skin keratinocytes and fibroblasts exposed to UVA and UVB radiation were treated with ascorbic acid or/and rutin. The total antioxidant properties of both components, as well as their effect on cellular pro-and antioxidant status, lipid and protein oxidation, transmembrane transport, and pro-inflammatory and pro/ antiapoptotic protein expression were measured. The combination of ascorbic acid and rutin had higher antioxidant properties compared to the activity of the single compound alone, and showed a stronger effect against UV-induced reactive oxygen species generation. The ascorbic acid and rutin combination also showed increased antioxidant enzyme activity (catalase, superoxide dismutase, thioredoxin reductase), which was impaired following UV irradiation. Moreover, ascorbic acid additional stimulated UV-induced bilitranslocase activity responsible for rutin transport, and therefore favored rutin effect on Nrf2 pathway, simultaneously differentiating the reaction of keratinocytes and fibroblasts. In keratinocytes, Nrf2 is strongly activated, while in fibroblasts decreased Nrf2 activity was observed. Used mixture, also significantly silenced UV-induced expression of pro-inflammatory factor NFκB and pro-apoptotic proteins such as caspases 3, 8, and 9. These results showed that ascorbic acid and rutin are complementary in their antioxidant actions, transport and signaling functions. Their combined antioxidant, antiinflammatory and antiapoptotic actions suggest rutin and ascorbic acid are a potentially cytoprotective team against UV-induced skin damage.

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“…135 ). The UVB protection of RU is also quite well documented in vitro and in vivo 134,[136][137][138][139] .…”

Section: Rutinmentioning

confidence: 94%

“…The RU pre-treatment of NHDF strongly protected against the UVA-induced increase in expression of proteins involved in antioxidant (such as SOD, TrxR, and peroxiredoxins 1/2) and inflammatory (e.g., IL-17, serine/ threonine-protein kinase 2, and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta) response 136 . RU also reduced the UVA-induced oxidative modification of the membrane of CCD 1112Sk human fibroblasts, specifically the negative charge on the membrane surface, sialic acid concentration and level of lipid peroxidation 137 .…”

Section: Rutinmentioning

confidence: 97%

Ultraviolet A protective potential of plant extracts and phytochemicals

Škařupová

Vostalová

Svobodová

2020

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Chronic exposure to solar radiation is related to an increased incidence of various skin disorders, including premature skin aging and melanoma and non-melanoma skin cancers. Ultraviolet (UV) photons in particular are responsible for skin damage. Solar UV photons mainly belong to UVA wavebands, however UVA radiation has been mostly ignored for a long time. At the cellular level, UVA photons mainly provoke indirect oxidative damage to biomolecules via the massive generation of unstable and highly reactive compounds. Human skin has several effective mechanisms that forestall, repair and eliminate damage caused by solar radiation. Regardless, some damage persists and can accumulate with chronic exposure. Therefore, conscious protection against solar radiation (UVB+UVA) is necessary. Besides traditional types of photoprotection such as sunscreen use, new strategies are being searched for and developed. One very popular protective strategy is the application of phytochemicals as active ingredients of photoprotection preparations instead of synthetic chemicals. Phytochemicals usually possess additional biological activities besides absorbing the energy of photons, and those properties (e.g. antioxidant, anti-inflammatory) magnify the protective potential of phytochemicals and extracts. Therefore, compounds of natural origin are in the interest of researchers as well as developers. In this review, only studies on UVA protection with well-documented experimental conditions are summarized. This article includes 17 well standardized plant extracts (Camellia sinensis (L.) Kuntze, Silybum marianum L. Gaertn., Punica granatum L., Polypodium aureum L., Vaccinium myrtillus L., Lonicera caerulea L., Thymus vulgaris L., Opuntia ficus-indica (L.) Mill., Morinda citrifolia L., Aloe vera (L.) Burm.f., Oenothera paradoxa Hudziok, Galinsoga parviflora Cav., Galinsoga quadriradiata Ruiz et Pavón, Hippophae rhamnoides L., Cola acuminata Schott & Endl., Theobroma cacao L. and Amaranthus cruentus L.) and 26 phytochemicals.

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Proteins involved in the antioxidant and inflammatory response in rutin-treated human skin fibroblasts exposed to UVA or UVB irradiation

Abstract: In conclusion, our results show that rutin effectively prevents UV-induced damages associated with proinflammatory and prooxidative activity and protects cells against apoptosis.

Cited by 35 publications

References 64 publications

Cytoprotective Effect of Ascorbic Acid and Rutin against Oxidative Changes in the Proteome of Skin Fibroblasts Cultured in a Three-Dimensional System

Cytoprotective Effect of Ascorbic Acid and Rutin against Oxidative Changes in the Proteome of Skin Fibroblasts Cultured in a Three-Dimensional System

Rutin and ascorbic acid cooperation in antioxidant and antiapoptotic effect on human skin keratinocytes and fibroblasts exposed to UVA and UVB radiation

Ultraviolet A protective potential of plant extracts and phytochemicals

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