Proteins involved in mitochondrial metabolic functions and fertilization predominate in stallions with better motility

Gaitskell‐Phillips, Gemma; Martín-Cano, Francisco E.; Ortiz-Rodríguez, J.M.; Silva-Rodríguez, Antonio; Silva-Álvarez, Eva da; Rojo-Domínguez, Patricia; Tapia, José A.; Gil, María Concepción Robles; Ortega-Ferrusola, Cristina; Peña, Fernando J.

doi:10.1016/j.jprot.2021.104335

Cited by 8 publications

(6 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent proteomics studies link higher amounts of the enzyme fructose bisphosphate aldolase and poor motility and velocities; this is the enzyme involved in splits of 1, 6 fructose bisphosphate in dihydroxyacetone phosphate (DHAP) and glyceraldehyde 3-phosphate (G3P), are precursors of G and MG (49). Production of G, and especially MG was concomitant with reduced motility and sperm velocities, a drop in mitochondrial membrane potential, and an increment of the production of ROS (49,125).…”

Section: Metabolic Disfunction Production Of Ros In the Spermatozoamentioning

confidence: 99%

The Stallion Spermatozoa: A Valuable Model to Help Understand the Interplay Between Metabolism and Redox (De)regulation in Sperm Cells

Peña

O’Flaherty

Rodriguez

et al. 2022

Antioxidants & Redox Signaling

Self Cite

View full text Add to dashboard Cite

Significance Proper functionality of the spermatozoa depends on the tight regulation of their redox status, at the same time these cells are very energy demanding, and in the energetic metabolism, principally in the electron transport chain (ETC) in the mitochondria, reactive oxygen species (ROS) are continuously produced, but also in the Krebs Cycle and during the beta-oxidation of fatty acids. Recent advances Additionally, in the glycolysis, elimination of phosphate groups from glyceraldehyde 3-phosphate and dihydroxyacetone phosphate originates as byproducts glyoxal (G) and methylglyoxal (MG); these products are 2oxoaldehydes. The presence of adjacent carbonyl groups make them strong electrophiles that react with nucleophiles of proteins, lipids and DNA, forming advanced glycation end products (AGEs). Critical Issues. This mechanism is behind subfertility in diabetic patients; in the animal breeding industry, commercial extenders for stallion semen contain a supraphysiological concentration of glucose that promotes methylglyoxal production, constituting a potential model of interest. Future directions. Increasing our knowledge on sperm metabolism and its interactions with redox regulation, may improve current sperm technologies in use, and shall provide new clues to for the understanding of infertility in males. Moreover, stallion spermatozoa due to its accessibility, intense metabolism, and suitability for proteomics/metabolomic studies may constitute a suitable model for studies of the regulation of metabolism and the interaction between metabolism and redox homeostasis.

show abstract

Section: Metabolic Disfunction Production Of Ros In the Spermatozoamentioning

confidence: 99%

The Stallion Spermatozoa: A Valuable Model to Help Understand the Interplay Between Metabolism and Redox (De)regulation in Sperm Cells

Peña

O’Flaherty

Rodriguez

et al. 2022

Antioxidants & Redox Signaling

Self Cite

View full text Add to dashboard Cite

show abstract

“…The separation and analysis were conducted in an Agilent 1290 Infinity II Series UHPLC (Agilent Technologies, Santa Clara, CA, USA) equipped with an automated multisampler module and a High Speed Binary Pump, coupled to a Mass Spectrometer (Agilent 6550 Q-TOF, Agilent Technologies, Santa Clara, CA, USA) using an Agilent Jet Stream Dual electrospray (AJS-Dual ESI) interface, controlled by the MassHunter Workstation Data Acquisition software (Agilent Technologies, Rev. B.06.01), following previously published protocols [ 2 , 4 , 5 , 9 ]. The digested proteins were injected onto an Agilent Advance Bio Peptide Mapping HPLC column (2.7 μm, 150 × 2.1 mm, Agilent technologies), at 55 °C, at a flow rate of 0.4 ml/min.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…This information, after re-analysis, may be used to improve our understanding of the regulation of stallion sperm motility, and to develop on farm tests to check sperm quality. The data set has been deposited to the ProteomeXchange Consortium [2] via the PRIDE partner repository with the dataset identifier PRIDE PXD025807 , https://www.ebi.ac.uk/pride/archive/projects/PXD025807…”

Section: Data Descriptionmentioning

confidence: 99%

Dataset of the sperm proteome of stallions with different motility

et al. 2022

Self Cite

View full text Add to dashboard Cite

“…A noteworthy finding of recent proteomic studies is a molecular explanation for the link between sperm motility and velocities, metabolism, and ability to fertilize [5]. In stallions showing better percentages of motility, circular and average velocity, mitochondrial proteins with roles in the citric acid cycle, pyruvate metabolism, and oxidative phosphorylation predominate.…”

Section: Proteomics In the Improvement Of Stallion Sperm Biotechnologiesmentioning

confidence: 99%

“…As far as the authors know, the first comprehensive study of the proteome of the stallion spermatozoa was conducted by Australian researchers at the University of Newcastle [2]. Since this first study, proteomic and phospho-proteomic approaches have been introduced to the study of stallion sperm biology [3][4][5][6][7][8][9][10][11]. This review aims to provide an updated overview of the major findings and advances in the comprehension of the stallion spermatozoa obtained thanks to the introduction of proteomics to the study of stallion sperm biology, and the impact of these findings on sperm biotechnology in equines.…”

mentioning

confidence: 99%

Proteomics is advancing the understanding of stallion sperm biology

Peña,

Martín‐Cano,

Becerro‐Rey

et al. 2024

Proteomics

Self Cite

View full text Add to dashboard Cite

The mammalian ejaculate is very well suited to proteomics studies. As such, research concerning sperm proteomics is offering a huge amount of new information on the biology of spermatozoa. Among domestic animals, horses represent a species of special interest, in which reproductive technologies and a sizeable market of genetic material have grown exponentially in the last decade. Studies using proteomic approaches have been conducted in recent years, showing that proteomics is a potent tool to dig into the biology of the stallion spermatozoa. The aim of this review is to present an overview of the research conducted, and how these studies have improved our knowledge of stallion sperm biology. The main outcomes of the research conducted so far have been an improved knowledge of metabolism, and its importance in sperm functions, the impact of different technologies on the sperm proteome, and the identification of potential biomarkers. Moreover, proteomics of seminal plasma and phosphoproteomics are identified as areas of major interest.

show abstract

Proteins involved in mitochondrial metabolic functions and fertilization predominate in stallions with better motility

Cited by 8 publications

References 62 publications

The Stallion Spermatozoa: A Valuable Model to Help Understand the Interplay Between Metabolism and Redox (De)regulation in Sperm Cells

The Stallion Spermatozoa: A Valuable Model to Help Understand the Interplay Between Metabolism and Redox (De)regulation in Sperm Cells

Dataset of the sperm proteome of stallions with different motility

Proteomics is advancing the understanding of stallion sperm biology

Contact Info

Product

Resources

About