Proteins involved in focal adhesion signaling pathways are differentially regulated in experimental branch retinal vein occlusion

Cehofski, Lasse Jørgensen; Kruse, Andreas; Kjærgaard, Benedict; Stensballe, Allan; Honoré, Bent; Vorum, Henrik

doi:10.1016/j.exer.2015.06.011

Cited by 23 publications

(24 citation statements)

References 45 publications

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“…Animal models of BRVO provide a unique opportunity to study proteome changes in RVO at the retinal level [11,31,46] and have revealed a number of proteins that were not previously associated with RVO. Using an experimental model of BRVO, our group [11] reported that laser-induced BRVO results in increased retinal levels of laminin subunit β-2, laminin subunit γ-1, lipocalin-7, nidogen-2, osteopontin, integrin-β1, isoform 2 of α-actinin-1, and talin-2.…”

Section: Retinal Vein Occlusionmentioning

confidence: 99%

“…Using an experimental model of BRVO, our group [11] reported that laser-induced BRVO results in increased retinal levels of laminin subunit β-2, laminin subunit γ-1, lipocalin-7, nidogen-2, osteopontin, integrin-β1, isoform 2 of α-actinin-1, and talin-2. These findings indicated that experimental BRVO was associated with focal adhesion signaling, integrin signaling and major extracellular matrix remodeling processes (Figure 5) that are likely to take place in close proximity to the occluded vessel.…”

Section: Retinal Vein Occlusionmentioning

confidence: 99%

“…Therefore, analyses with different proteomic techniques are likely to provide further insights into proteome changes in the macular region in AMD if macular tissue can be collected from human donors post-mortem. For example, techniques like label-free LC-MS/MS and tandem mass tags require only small amounts of retinal tissue [11,31] which make them well-suited for proteomic studies of the macular region. Future studies may also address retinal proteome changes following intravitreal anti-VEGF treatment from eyes collected post-mortem, but such donor eyes may be difficult to obtain.…”

Section: Age-related Macular Degenerationmentioning

confidence: 99%

“…However, discovery proteomics does not guarantee identification of specific key proteins. This is the case for a number of growth factors including VEGF, platelet-derived growth factor and placental growth factor which are rarely detected in discovery proteomics [11,31]. However, recent studies have proved that receptors for growth factors can be identified in experiments using discovery proteomics.…”

Section: Limitations In Proteomicsmentioning

confidence: 99%

“…Large-scale protein studies using proteomic techniques have proved very effective in generating important knowledge about the complex pathological mechanisms underlying RVO, DR and AMD [10,11]. Furthermore, intraocular therapeutic targets have been identified with proteome studies [10].…”

Section: Introductionmentioning

confidence: 99%

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A Review: Proteomics in Retinal Artery Occlusion, Retinal Vein Occlusion, Diabetic Retinopathy and Acquired Macular Disorders

Cehofski

Honoré

Vorum

2017

IJMS

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Retinal artery occlusion (RAO), retinal vein occlusion (RVO), diabetic retinopathy (DR) and age-related macular degeneration (AMD) are frequent ocular diseases with potentially sight-threatening outcomes. In the present review we discuss major findings of proteomic studies of RAO, RVO, DR and AMD, including an overview of ocular proteome changes associated with anti-vascular endothelial growth factor (VEGF) treatments. Despite the severe outcomes of RAO, the proteome of the disease remains largely unstudied. There is also limited knowledge about the proteome of RVO, but proteomic studies suggest that RVO is associated with remodeling of the extracellular matrix and adhesion processes. Proteomic studies of DR have resulted in the identification of potential therapeutic targets such as carbonic anhydrase-I. Proliferative diabetic retinopathy is the most intensively studied stage of DR. Proteomic studies have established VEGF, pigment epithelium-derived factor (PEDF) and complement components as key factors associated with AMD. The aim of this review is to highlight the major milestones in proteomics in RAO, RVO, DR and AMD. Through large-scale protein analyses, proteomics is bringing new important insights into these complex pathological conditions.

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Section: Retinal Vein Occlusionmentioning

confidence: 99%

Section: Retinal Vein Occlusionmentioning

confidence: 99%

Section: Age-related Macular Degenerationmentioning

confidence: 99%

Section: Limitations In Proteomicsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A Review: Proteomics in Retinal Artery Occlusion, Retinal Vein Occlusion, Diabetic Retinopathy and Acquired Macular Disorders

Cehofski

Honoré

Vorum

2017

IJMS

Self Cite

View full text Add to dashboard Cite

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The Pig PeptideAtlas: A resource for systems biology in animal production and biomedicine

et al. 2016

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Biological research of Sus scrofa, the domestic pig, is of immediate relevance for food production sciences, and for developing pig as a model organism for human biomedical research. Publicly available data repositories play a fundamental role for all biological sciences, and protein data repositories are in particular essential for the successful development of new proteomic methods. Cumulative proteome data repositories, including the PeptideAtlas, provide the means for targeted proteomics, system wide observations, and cross species observational studies, but pigs have so far been underrepresented in existing repositories. We here present a significantly improved build of the Pig PeptideAtlas, which includes pig proteome data from 25 tissues and three body fluid types mapped to 7139 canonical proteins. The content of the Pig PeptideAtlas reflects actively ongoing research within the veterinary proteomics domain, and this manuscript demonstrates how the expression of isoform-unique peptides can be observed across distinct tissues and body fluids. The Pig PeptideAtlas is a unique resource for use in animal proteome research, particularly biomarker discovery and for preliminary design of SRM assays, which are equally important for progress in research that supports farm animal production and veterinary health, as for developing pig models with relevance to human health research.

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Retinal proteome changes following experimental branch retinal vein occlusion and intervention with ranibizumab

Cehofski

Kruse

Bøgsted

et al. 2016

Experimental Eye Research

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Proteins involved in focal adhesion signaling pathways are differentially regulated in experimental branch retinal vein occlusion

Cited by 23 publications

References 45 publications

A Review: Proteomics in Retinal Artery Occlusion, Retinal Vein Occlusion, Diabetic Retinopathy and Acquired Macular Disorders

A Review: Proteomics in Retinal Artery Occlusion, Retinal Vein Occlusion, Diabetic Retinopathy and Acquired Macular Disorders

The Pig PeptideAtlas: A resource for systems biology in animal production and biomedicine

Retinal proteome changes following experimental branch retinal vein occlusion and intervention with ranibizumab

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