Proteins in the uterine secretions of the cow

Dixon, S. N.; Gibbons, R. A.

doi:10.1530/jrf.0.0560119

Cited by 26 publications

(11 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The function of this protein is to transport iron from the endometrium to the conceptus (Chen et al, 1975 ;Roberts et al, 1986) (Roberts and Parker, 1974). However, specific uterine proteins have been described in cows (Roberts and Parker, 1974 ;Laster, 1977 ;Dixon and Gibbons, 1979) and in sheep (Roberts G. P. et al, 1976;Staples et al, 1978). Up to now, none of these proteins have been isolated and purified.…”

Section: Introductionmentioning

confidence: 99%

Embryo-uterine interactions during early stages of pregnancy in domestic mammals

Guillomot¹,

Reinaud²,

Charpigny³

et al. 1988

Reprod. Nutr. Dévelop.

View full text Add to dashboard Cite

Summary. The We also studied the effects of co-culture with uterine cells on blastocyst DNA and protein synthesis. In no cases we obtained stimulation of blastocyst development during the co-culture period, and DNA or protein synthesis decreased in the presence of uterine cells.In conclusion, the presence of specific uterine proteins has been established in some domestic mammals (pig, rabbit) but not in all of them. Although local modifications of uterine protein synthesis are induced by the embryo or its secretory products, the nature and the role of the proteins which are affected need to be determined by further studies.Introduction.

show abstract

Section: Introductionmentioning

confidence: 99%

Embryo-uterine interactions during early stages of pregnancy in domestic mammals

Guillomot¹,

Reinaud²,

Charpigny³

et al. 1988

Reprod. Nutr. Dévelop.

View full text Add to dashboard Cite

show abstract

“…Lee and Ax [20] have reported that the concentrations of GAGs per 100 mg of protein identified from flushing of bovine oviduct and uterine horn are 9 and 20 mg/ml, respectively. The total protein concentrations for oviductal and uterine fluid are 1.2-52.0 [52,53] and approximately 10.0-18.6 mg/ml, respectively [54,55]. Therefore, the total GAG concentration in oviductal and uterine fluid is estimated at approximately 0.1-4.7 and 2.0-3.7 mg/ml, respectively.…”

Section: Discussionmentioning

confidence: 99%

Effects of Glycosaminoglycans on the Development of in Vitro-Matured and -Fertilized Porcine Oocytes to the Blastocyst Stage in Vitro1

Kano

Miyano

Kato

1998

Biology of Reproduction

View full text Add to dashboard Cite

We examined the effects of four glycosaminoglycans (GAGs) on the development of in vitro-matured (IVM) and -fertilized (IVF) porcine oocytes to the blastocyst stage. IVM and IVF oocytes were cultured in Whitten's medium supplemented with hyaluronic acid, chondroitin sulfate A, dermatan sulfate, or heparin at 38.5 degrees C in an atmosphere of 5% CO2 in humidified air for up to 6 days. After 2 days in culture, 28-34% of the inseminated oocytes cleaved to the 2- to 8-cell stage, and the GAGs showed no significant effect on development. After 6 days in culture, blastocysts were observed in all groups. The percentage of blastocysts was significantly higher in hyaluronic acid-supplemented medium (14%) than in dermatan sulfate-supplemented (5%), heparin-supplemented (2%), or nonsupplemented (2%) media. In addition, the percentage of blastocysts was significantly higher in chondroitin sulfate A-supplemented medium (11%) than in heparin-supplemented and nonsupplemented media, although the number of blastocysts in chondroitin sulfate A was not significantly different from that in hyaluronic acid- and dermatan sulfate-supplemented media. There were no significant differences in the mean number of nuclei per blastocyst cultured in any group. The effects of hyaluronic acid and chondroitin sulfate A on development to the blastocyst stage was examined at various concentrations. After 6 days in culture, development of IVM and IVF oocytes to the blastocyst stage was best supported in 0.5 mg/ml hyaluronic acid-supplemented (17%) and in 0.1 or 0.5 mg/ml chondroitin sulfate A-supplemented (10% or 9%, respectively) media. It is concluded from these results that hyaluronic acid and chondroitin sulfate A supported the development of porcine oocytes matured and fertilized in vitro to the blastocyst stage.

show abstract

“…Similarly, the Pinduced uterine milk proteins accumulate in the uterine lumen of ewes and cows in significant amounts only after prolonged P exposure [7,11], suggesting that a similar scenario may be true for RBP. In addition to RBP, lactoferrin is another bovine endometrial transport protein that is regulated by P [4].…”

Section: Discussionmentioning

confidence: 99%

“…In fact, since conceptuses of large domestic farm animals undergo noninvasive epitheliochorial placentation and the uterine epithelium is never eroded during pregnancy, uterine secretions may be required for nutritional support of the conceptus throughout gestation [1]. For this reason, uterine secretions of cattle [3][4][5][6][7][8], sheep [9][10][11][12], and pigs [13][14][15][16][17] have received considerable attention.…”

Section: Introductionmentioning

confidence: 99%

“…The majority of protein production by uterine endometrium and transport of serum-derived proteins into the uterine lumen occurs during the luteal phase of the estrous cycle, in pregnancy, or in response to exogenous progesterone (P) in cattle [3,4,6,7], sheep [9,11,18], and pigs [13,14,19]. Although P is responsible for stimulating synthesis of proteins by the uterine endometrium, estrogen (E) stimulates rapid accumulation of uterine luminal protein in cows [5] and pigs [17,20,21].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Retinol-Binding Protein Gene Expression in Cyclic and Pregnant Endometrium of Pigs, Sheep, and Cattle1

Harney

Ott

Geisert

et al. 1993

Biology of Reproduction

View full text Add to dashboard Cite

Retinol-binding protein (RBP) is a major secretory product of conceptuses and endometrium of the large domestic animals. The present study examined the abundance of RBP mRNA in cyclic and early pregnant endometrium of pigs, sheep, and cattle and confirmed the presence of RBP mRNA in preiimplantation conceptuses of the large domestic animals. Northern blot analysis, using porcine liver RBP cDNA as the probe, detected a 1.0-1.1-kb transcript in conceptuses and endometrium collected during the preiimplantation period of pregnancy in pigs (Day 15), sheep (Day 16), and cows (Day 18). Slot-blot analysis of RBP mRNA in endometrium of pigs, sheep, and cows indicated differential RBP gene expression across days and statuses in pigs and sheep and across days alone in cows. In pigs, RBP gene expression was low to undetectable at Days 0, 5, and 10 of the estrous cycle and Day 10 of pregnancy. Levels of RBP mRNA increased (p < 0.06) from Days 10 to 12 and further (p < 0.001) to Day 15 across both statuses. At Day 18, RBP mRNA levels decreased (p < 0.01) in cyclic pigs but remained elevated in endometrium of pregnant pigs. In sheep, levels of RBP mRNA increased between Days 10 and 12 and 14 in both cyclic and pregnant ewes; however, between Days 14 and 16, RBP mRNA levels remained elevated in cyclic endometrium but decreased (p < 0.01) in endometrium of pregnant ewes. In a second experiment, RBP mRNA levels increased (p < 0.01) between Days 0-5 and Days 13-15 of the estrous cycle.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

Proteins in the uterine secretions of the cow

Cited by 26 publications

References 48 publications

Embryo-uterine interactions during early stages of pregnancy in domestic mammals

Embryo-uterine interactions during early stages of pregnancy in domestic mammals

Effects of Glycosaminoglycans on the Development of in Vitro-Matured and -Fertilized Porcine Oocytes to the Blastocyst Stage in Vitro1

Retinol-Binding Protein Gene Expression in Cyclic and Pregnant Endometrium of Pigs, Sheep, and Cattle1

Contact Info

Product

Resources

About