Proteins in the periplasmic space and outer membrane vesicles ofRhizobium etliCE3 grown in minimal medium are largely distinct and change with growth phase

Abstract

3Rhizobium etli CE3 grown in succinate-ammonium minimal medium (MM) excreted 4 outer membrane vesicles (OMVs) with diameters of 40 to 100 nm. Proteins from the 5 OMVs and the periplasmic space were isolated from 6 and 24 h cultures and identified 6 by proteome analysis. A total 770 proteins were identified: 73.8 and 21.3 % of these 7 proteins occurred only in the periplasm and OMVs, respectively, and only 4.9 % were 8 found in both locations. The majority of proteins found in either location were present 9 only at 6 or 24 h: in the periplasm and OMVs, only 24 and 9 % of proteins, respectively,