Proteins Entrapped in Silica Monoliths Prepared from Glyceroxysilanes

Brook, Michael A.; Yang, Chen; Guo, Kui; Zhang, Zheng; Jin, Wen; Deisingh, Anil K.; Cruz-Aguado, Jorge A.; Brennan, John D.

doi:10.1023/b:jsst.0000048014.09497.39

Cited by 57 publications

(59 citation statements)

References 11 publications

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“…Some of the common hydrophilic substitutions include glasses modified with aminopropyl [48][49][50][51], carboxyethyl [48,49], glycidoxypropyl [50,51], and diglyceryl or gluconamidyl groups [52][53][54]. Of special interest with regard to hydrophilic organically-modified glasses, Ackerman and co-workers have reported outstanding enzyme activities following protein adsorption into functionalized mesoporous silica prepared using a non-ionic block copolymer surfactant as the template [48,49].…”

Section: Discussionmentioning

confidence: 99%

Favourable influence of hydrophobic surfaces on protein structure in porous organically-modified silica glasses

et al. 2008

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Organically-modified siloxanes were used as host materials to examine the influence of surface chemistry on protein conformation in a crowded environment. The sol-gel materials were prepared from tetramethoxysilane and a series of monosubstituted alkoxysilanes, RSi(OR′) 3 , featuring alkyl groups of increasing chain length in the R-position. Using circular dichroism spectroscopy in the far-UV region, apomyoglobin was found to transit from an unfolded state to a native-like helical state as the content of the hydrophobic precursor increased from 0-15%. At a fixed molar content of 5% RSi(OR') 3 , the helical structure of apomyoglobin increased with the chain length of the R-group, i.e. methyl < ethyl < n-propyl < n-butyl < n-hexyl. This trend also was observed for the tertiary structure of ribonuclease A, suggesting that protein folding and biological activity are sensitive to the hydrophilic/hydrophobic balance of neighboring surfaces. The observed changes in protein structure did not correlate with total surface area or the average pore size of the modified glasses, but scanning electron microscopy images revealed an interesting relationship between surface morphology and alkyl chain length. The unexpected benefit of incorporating a low content of hydrophobic groups into a hydrophilic surface may lead to materials with improved biocompatibility for use in biosensors and implanted devices.

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Section: Discussionmentioning

confidence: 99%

Favourable influence of hydrophobic surfaces on protein structure in porous organically-modified silica glasses

et al. 2008

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“…According to the literature, GOPS should react with glycerol via an epoxy-hydroxy (etherification) reaction, to form a network structure. 43,44 Alternative or parallel reaction paths can be the condensation of the methoxy groups with the -OH groups of glycerol 45,46 and at the glass substrate surface. 47 It is worth mentioning that we observed a thickness increase and an identical thickness before and after water immersion also for films processed from PEDOT:PSS/GOPS mixtures in the absence of glycerol.…”

Section: 51mentioning

confidence: 99%

Solvent-induced changes in PEDOT:PSS films for organic electrochemical transistors

et al. 2014

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Organic electrochemical transistors based on the conducting polymer poly(3,4-ethylenedioxythiophene) doped with poly(styrenesulfonate) (PEDOT:PSS) are of interest for several bioelectronic applications. In this letter, we investigate the changes induced by immersion of PEDOT:PSS films, processed by spin coating from different mixtures, in water and other solvents of different polarities. We found that the film thickness decreases upon immersion in polar solvents, while the electrical conductivity remains unchanged. The decrease in film thickness is minimized via the addition of a cross-linking agent to the mixture used for the spin coating of the films.

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“…In addition, alkoxysilanes can be used to introduce functionalities for later derivatisation of the stationary phase or for direct tailoring of chromatographic properties. The following alkoxysilanes have been used separately or in combinations: tetraethoxysilane (TEOS) [30], methyltrimethoxysilane (MTMS) [31], ethoxytrimethoxysilane (ETMS) [32], 2-cyanoethyltriethoxysilane (CEOS) [33], (3-aminopropyl) triethoxysilane (APTES) [34] and diglycerylsilane (DGS) [30,34]. Table 1 summarises the effects different synthetic parameters have on the monolith structure, and Table 2 gives an overview over other reagents that have been used in the preparation of monoliths in order to vary their bimodal structure and/or their selectivity.…”

Section: Synthesismentioning

confidence: 99%

Silica monolithic columns: Synthesis, characterisation and applications to the analysis of biological molecules

Rieux

Niederländer

Verpoorte

et al. 2005

J of Separation Science

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In recent years, proteomics has been a subject of intense research. The complexity of proteomics samples has fostered technological developments. One of these addresses the need for more efficient and faster separations. Monolithic columns prepared from organic and silica monomers offer very efficient separations at low back-pressure. Silica-based monoliths have small-sized skeletons and a bimodal pore size distribution with microm-sized throughpores and nm-sized mesopores. This gives silica-based monoliths favourable properties for high-efficiency, fast separations, like a low-pressure drop across the column, fast mass transfer kinetics and a high binding capacity.

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Proteins Entrapped in Silica Monoliths Prepared from Glyceroxysilanes

Cited by 57 publications

References 11 publications

Favourable influence of hydrophobic surfaces on protein structure in porous organically-modified silica glasses

Favourable influence of hydrophobic surfaces on protein structure in porous organically-modified silica glasses

Solvent-induced changes in PEDOT:PSS films for organic electrochemical transistors

Silica monolithic columns: Synthesis, characterisation and applications to the analysis of biological molecules

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