Proteins differentially expressed in elicited cell suspension culture of Podophyllum hexandrum with enhanced podophyllotoxin content

Bhattacharyya, Dipto; Sinha, Ragini; Ghanta, Srijani; Chakraborty, Amrita; Hazra, Saptarshi; Chattopadhyay, Sharmila

doi:10.1186/1477-5956-10-34

Cited by 50 publications

(29 citation statements)

References 74 publications

(82 reference statements)

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“…Treatment of the leaves of the P. hexandrum plant with methyl jasmonate indeed increased the podophyllotoxin production in the roots by 21%. This observation is in line with the findings of Bhattacharyya in cell cultures of P. hexandrum (Bhattacharyya et al 2012). As previously observed by Baldwin, treatment of the leaves triggers the secondary metabolite production (nicotine) in the roots (Baldwin 1996).…”

Section: Discussionsupporting

confidence: 79%

“…Most elicitation studies have been performed on cell suspension and hairy root cultures (van der Fits 2000; Häkkinen et al 2004;Baldi and Dixit 2008;Todd et al 2010;Shoji et al 2010;Suttipanta et al 2011). Also the podophyllotoxin production in P. hexandrum suspension cultures was increased by seven to eight-fold after stimulation with methyl jasmonate (Bhattacharyya et al 2012). Furthermore, methyl jasmonate treatment is also effective in vivo, as treatment of Nicotiana attenuata leaves increased nicotine production in the roots (Baldwin 1996).…”

Section: Introductionmentioning

confidence: 99%

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Methyl jasmonate treatment increases podophyllotoxin production in Podophyllum hexandrum roots under glasshouse conditions

et al. 2017

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Background and aim The endangered Podophyllum hexandrum is an important industrial source of podophyllotoxin, which is a precursor for the anticancer drugs etoposide and teniposide. Attempts to obtain podophyllotoxin through cell cultures or chemical synthesis have still a long way to go before being economical feasible. The objective of this study was to increase the root formation and podophyllotoxin production of P. hexandrum cultivated in a glasshouse. Methods Root formation and podophyllotoxin production of P. hexandrum in sand or peat-perlite soil at 15°C or 25°C was determined. Furthermore, the influence of methyl jasmonate on the podophyllotoxin production was determined. Results More root formation was observed in peat-perlite soil than in sand soil. Furthermore, root formation was higher at 15°C than at 25°C. This resulted in the highest podophyllotoxin production per plant in peat-perlite at 15°C (160 ± 22 mg/plant d.w.). Furthermore, methyl jasmonate treatment of the leaves increased the podophyllotoxin production in the roots by 21%. Conclusion We were able to cultivate P. hexandrum in a glasshouse in the Netherlands and improve the root formation and podophyllotoxin production. This paves the way for large-scale cultivation of P. hexandrum in the temperate latitudes for the production of the pharmaceutical interesting podophyllotoxin.

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Section: Discussionsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Methyl jasmonate treatment increases podophyllotoxin production in Podophyllum hexandrum roots under glasshouse conditions

et al. 2017

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“…The growth decline under MeJa treatment was further reported in cell suspension cultures of T. x. media carrying the additional txs gene by Onrubia et al (2012). The results of studies on the exploration of the protein profile of MeJa elicited cell suspension cultures of Podophyllum hexandrum, revealed the up-accumulation of defense/stress-related protein, proteins engaged in secondary metabolites production and signal transduction (Bhattacharyya et al 2012). However, the authors also noted the down-regulation of some proteins involved in primary cell metabolism, for example cyclin A protein connected with the cell division cycle which could be correlated with cell growth suppression caused by MeJa.…”

Section: Pfd-phases Supported Cultures Of Atma and Kt Hairy Root Linesmentioning

confidence: 88%

“…However, on day 35 of cultivation, one week after elicitors addition, both fresh and dry biomass increase was higher in PFD-supported elicited cultures. This phenomena could be connected with the acting of MeJa activating primary and secondary metabolism which was discussed on the proteome level (Bhattacharyya et al 2012). …”

Section: Pfd-phases Supported Cultures Of Atma and Kt Hairy Root Linesmentioning

confidence: 99%

Lignan accumulation in two-phase cultures of Taxus x media hairy roots

Sykłowska-Baranek

Łysik

Jeziorek

et al. 2018

Plant Cell Tiss Organ Cult

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The biosynthetic potential for six lignans accumulation in two lines of Taxus x media hairy roots was investigated. The cultures of KT and ATMA hairy root lines were supplemented with precursors: coniferyl alcohol (CA 1, 10 or 100 µM) and/or l-phenylalanine (100 µM PHEN) and/or methyl jasmonate (100 µM MeJa). Moreover the two-phase in vitro cultures supported with perfluorodecalin (PFD) as a gas carrier and in situ extrahent were used. The hairy root lines differed in lignan production profiles. In the control untreated cultures KT roots did not accumulate secoisolariciresinol and lariciresinol while ATMA roots did not accumulate matairesinol. In ATMA roots the treatment with CA (1 or 10 µM) resulted in the production of lariciresinol and secoisolariciresinol whereas solely lariciresinol was present after 100 µM CA application. Elicitation with 1 µM CA and MeJa yielded with hydroxymatairesinol aglyca and lariciresinol glucosides with their highest content 37.88 and 3.19 µg/g DW, respectively. The stimulatory effect of simultaneous treatment with 1 µM CA, PHEN and MeJa on lignan production was observed when the cultures were supplemented with PFD-aerated or degassed. In ATMA root cultures these applied conditions were the most favourable for matairesinol content which amounted to 199.86 and 160.25 µg/g DW in PFD-aerated and PFD-degassed supported cultures, respectively. In KT root cultures solely, hydroxymatairesinol and coniferin/CA content was enhanced with their highest yield 59.29 and 134.60 µg/g DW in PFD-aerated and PFD-degassed cultures, respectively.

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“…Proteome analysis of potato tuberization showed induction of ROS detoxifying enzymes such as SOD, APX, and catalase, suggesting their possible role in tuber formation (Agrawal et al 2008b). In addition to potato tuberization, changes in Podophyllum hexandrum cell proteome potentially related to podophyllotoxin (PTOX) accumulation in response to methyl jasmonate (MeJA) elicitation has been investigated (Bhattacharayya et al 2012). PTOX is derived from the phenolpropanoid pathway and is the precursor for cancer therapeutics like etoposide, teniposide, and etophos.…”

Section: Growth and Developmentmentioning

confidence: 99%

Plant proteomics in India and Nepal: current status and challenges ahead

Deswal¹,

Gupta²,

Dogra³

et al. 2013

Physiol Mol Biol Plants

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Plant proteomics has made tremendous contributions in understanding the complex processes of plant biology. Here, its current status in India and Nepal is discussed. Gelbased proteomics is predominantly utilized on crops and noncrops to analyze majorly abiotic (49 %) and biotic (18 %) stress, development (11 %) and post-translational modifications (7 %). Rice is the most explored system (36 %) with major focus on abiotic mainly dehydration (36 %) stress. In spite of expensive proteomics setup and scarcity of trained workforce, output in form of publications is encouraging. To boost plant proteomics in India and Nepal, researchers have discussed ground level issues among themselves and with the International Plant Proteomics Organization (INPPO) to act in priority on concerns like food security. Active collaboration may help in translating this knowledge to fruitful applications. Ground-level issues in agriculture, biodiversity and sustainabilityThe Green revolution of the sixties led agricultural practices in the Indian subcontinent to improve, and fulfil the everincreasing demand for plant-based foods. This was concomitant with the consumption of large doses of inorganic fertilizers and chemical pesticides to sustain crop productivity. Despite the transformation of India into a "bread basket", a negative aspect of the then generally beneficial model, was the erosion of crop biodiversity through increasing industrialization of agriculture. This also gave rise to other ecological problems, such as decreasing soil fertility, chemical pollution of land and water resources, pesticide poisoning, and pest infestation due to growing pest resistance to pesticides. Agricultural crops or domesticated plants went through cycles of selections depending on needs and important traits. The traits kept changing as per the demands of particular eco-geography and/or demography, while the cycle of selection continued with limited number of genotypes in particular crop species. This practice resulted in a narrow primary or secondary gene pool of crop plants and their related species causing breeding depression, low polymorphism, and allied problems. Therefore, harnessing the available diversity only within crossable genotypes and/or species now limits conventional breeding. In fact, agriculture on the Indian subcontinent is once again at the crossroads. Further development of sustainable solutions will require deeper understanding of traits at molecular level that are critical in exploring and exploiting the existing molecular diversity in native plants. Molecular understanding of fertility barriers would be equally critical to utilize the well characterized, wider germplasm collections readily available for cereal crops.Managing biotic and abiotic stresses in crop production remains a huge challenge. This is compounded with the perpetually rising numbers of pests and pathogens due to changing climate and pest/pathogen adaptation to chemicals. Harnessing the existing biodiversity in crop plants using their proximate specie...

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Proteins differentially expressed in elicited cell suspension culture of Podophyllum hexandrum with enhanced podophyllotoxin content

Cited by 50 publications

References 74 publications

Methyl jasmonate treatment increases podophyllotoxin production in Podophyllum hexandrum roots under glasshouse conditions

Methyl jasmonate treatment increases podophyllotoxin production in Podophyllum hexandrum roots under glasshouse conditions

Lignan accumulation in two-phase cultures of Taxus x media hairy roots

Plant proteomics in India and Nepal: current status and challenges ahead

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