Proteins and Saccharides of the Sea Urchin Organic Matrix of Mineralization: Characterization and Localization in the Spine Skeleton

Ameye, Laurent; Becker, Geneviève De; Killian, Christopher E.; Wilt, Fred H.; Kemps, Raymond; Kuypers, Stephan; Dúbois, Philippe

doi:10.1006/jsbi.2001.4361

Cited by 50 publications

(38 citation statements)

References 55 publications

(61 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In calcareous sponges, different growth dynamics of spicule correspond to a spatial heterogeneity in cell type or activity (apical cell forming a primary nucleation site and a thickener cell spatially constraining lateral growth Ilan et al, 1996). Ameye et al (2001) have shown that within stereom trabeculae of sea urchin P. lividus, organic matrix proteinaceous components are localized within different sub-regions of the spine skeleton: N-glycoproteins are associated with the inner core of stereom bar, whereas O-glycoproteins localized in outer surface of trabeculae, where skeletal growth is largely inhibited by comparison. Furthermore, Aizenberg et al (1997) reported that the crystal texture differs between the meshwork stereom and the compact septa.…”

Section: Discussionmentioning

confidence: 99%

26Mg labeling of the sea urchin regenerating spine: Insights into echinoderm biomineralization process

Gorzelak

Stolarski

Dúbois

et al. 2011

Journal of Structural Biology

Self Cite

View full text Add to dashboard Cite

26Mg labeling NanoSIMS SEM a b s t r a c t This paper reports the results of the first dynamic labeling experiment with regenerating spines of sea urchins Paracentrotus lividus using the stable isotope 26 Mg and NanoSIMS high-resolution isotopic imaging, which provide a direct information about the growth process. Growing spines were labeled twice (for 72 and 24 h, respectively) by increasing the abundance of 26 Mg in seawater. The incorporation of 26 Mg into the growing spines was subsequently imaged with the NanoSIMS ion microprobe. Stereom trabeculae initially grow as conical micro-spines, which form within less than 1 day. These micro-spines fuse together by lateral outgrowths and form a thin, open meshwork (inner stereom), which is subsequently reinforced by addition of layered thickening deposits (outer stereom). The (longitudinal) growth rate of the inner stereom is ca. 125 lm/day. A single (ca. 1 lm) thickening layer in the stereom trabeculae is deposited during 24 h. The thickening process is contemporaneous with the formation micro-spines and involves both longitudinal trabeculae and transverse bridges to a similar degree. Furthermore, the skeleton-forming cells remain active in the previously formed open stereom for at least 10 days, and do not migrate upwards until the end of the thickening process. The experimental capability presented here provides a new way to obtain detailed information about the skeleton formation of a multitude of marine, calcite producing organisms.

show abstract

Section: Discussionmentioning

confidence: 99%

26Mg labeling of the sea urchin regenerating spine: Insights into echinoderm biomineralization process

Gorzelak

Stolarski

Dúbois

et al. 2011

Journal of Structural Biology

Self Cite

View full text Add to dashboard Cite

show abstract

“…[4][5][6][7][8][9][10][11] However, more investigations are still needed to elucidate the mechanisms by which such additives affect the CaCO 3 nanocrystals structures and their aggregation to yield various morphologies of CaCO 3 final particles. Thus, the aim of the present work is to throw light on the mechanism by which polyelectrolytes affect the crystalline structure, the size and the shape of CaCO 3 particles.…”

Section: Introductionmentioning

confidence: 99%

Role of Polyelectrolytes in Crystallogenesis of Calcium Carbonate

Jada

Jradi

2006

Macromolecular Symposia

View full text Add to dashboard Cite

Summary:The effects anionic polyelectrolytes, having various molecular weights and repeating unit structures, on the crystallization of calcium carbonate in supersaturated solutions are studied. The induction times of the crystals grown in the presence of the polymers were optically evaluated; X-ray diffraction and Scanning Electronic Microscopy (SEM) analyses were performed to determine, respectively, their crystalline structures and morphologies. The polyelectrolyte is found to lengthen the induction time and to reduce the size of CaCO 3 nanocrystallites, to an extent depending on the interaction efficiency between the polymer anionic repeating units and the calcium ions. Further, depending on their sizes and their crystalline structures (calcite, vaterite) the nanocrystallites aggregate and yield final calcium carbonate particles having various sizes and morphologies. The data indicate that nanocrystals having vaterite structure, as determined by X-ray analysis, give spherical CaCO 3 final particles, while nanocrystals having calcite structure lead to either acicular or flower shapes of CaCO 3 final particles.

show abstract

“…24 Various organic macromolecules, present in the spines and in the test plates, being attached to particular crystallographic planes, will result in different lattice distortions.…”

Section: Lattice Distortions and Intra-crystalline Organicsmentioning

confidence: 99%

“…22 Proteins in sea urchin skeletal elements have been extensively characterized. 20,21,23 Besides some glycoproteins and monosaccharides have been identified 22,24 and the presence of organic pigments was also reported. 20,25 Although sea urchin skeletal elements behave as single crystals in polarized light and X-ray diffraction, 26 they show conchoidal fracture-surfaces, 27,28 which fundamentally differ from the atomically flat ones produced in geological calcite by cleavage.…”

Section: Introductionmentioning

confidence: 99%

Interplay between Calcite, Amorphous Calcium Carbonate, and Intracrystalline Organics in Sea Urchin Skeletal Elements

Albéric

Caspi²,

Bennet

et al. 2018

Crystal Growth & Design

View full text Add to dashboard Cite

Biomineralization processes in living organisms result in the formation of skeletal elements with complex ultrastructures. Although the formation pathways in sea urchin larvae are known, the interrelation between calcite, amorphous calcium carbonate (ACC), and intra-crystalline organics in adult sea urchin biominerals is less clear. Here, we study this interplay in the spines and test 2 plates of the Paracentrotus lividus sea urchins whose skeletal elements have optimized functionproperties relationships. Thermogravimetric analysis coupled with differential scanning calorimetry or mass spectrometry measurements, nuclear magnetic resonance technique and high-resolution powder X-ray diffraction show that pristine spines and test plates are composed of Mg-rich calcite and comprise about 10 wt. % of anhydrous ACC, 1.2 to 1.6 wt. % of organics, and less than 0.2 wt. % of water. Anhydrous ACC originates from incomplete crystallization of a precursor ACC phase during biomineralization and is associated with intra-crystalline organics at the molecular level. Molecular interactions at organic/inorganic interfaces cause significant calcite lattice distortions of the tensile type. The latter are amplified during ACC crystallization and finally disappear after heat-assisted destruction of organic molecules. Converting the measured lattice distortions (strains) into internal stress components, we follow stress evolution upon annealing and find that complete crystallization of ACC leads to the isotropy of residual stresses in all investigated skeletal parts. These results allow us to speculate that organic macromolecules are preferentially attached to different crystallographic planes in the pristine test and spine samples.

show abstract

Proteins and Saccharides of the Sea Urchin Organic Matrix of Mineralization: Characterization and Localization in the Spine Skeleton

Cited by 50 publications

References 55 publications

26Mg labeling of the sea urchin regenerating spine: Insights into echinoderm biomineralization process

26Mg labeling of the sea urchin regenerating spine: Insights into echinoderm biomineralization process

Role of Polyelectrolytes in Crystallogenesis of Calcium Carbonate

Interplay between Calcite, Amorphous Calcium Carbonate, and Intracrystalline Organics in Sea Urchin Skeletal Elements

Contact Info

Product

Resources

About