Proteins and proteomics: life on the surface

Blow, Nathan

doi:10.1038/nmeth0509-389

Cited by 40 publications

(26 citation statements)

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“…In the past decades, metallic nanoparticles either sustained on surfaces or dispersed in dielectric matrices have been extensively studied because of their pronounced optical and electrical properties [1][2][3][4][5][6][7][8]. Most of the studies have focused on localized surface plasmon excitation, which dominates the photoabsorption spectra in the visible range.…”

Section: Introductionmentioning

confidence: 99%

“…Most of the studies have focused on localized surface plasmon excitation, which dominates the photoabsorption spectra in the visible range. In recent years, interest in the synthesis of dielectric-matrix-based bimetallic nanocomposites has intensified because of their considerable applications in nano-optical devices, especially in localized surface plasmon resonance spectroscopy and biosensing [3,[5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

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Shift of localized surface plasmon resonance by Ar-ion irradiation of Ag–Au bimetallic films deposited on Al2O3 single crystals

Meng

Shibayama

et al. 2013

Nuclear Instruments and Methods in Physics Research Section B:

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Effects of Ar-ion induced surface nanostructuring were studied using 100 keV Ar-ion irradiation of 30 nm Ag-Au bimetallic films deposited on Al 2 O 3 single crystals, under irradiation fluences ranging from 5.0 × 10 15 cm -2 to 6.3 × 10 16 cm -2 . Scanning electron microscope was used to study the ionbeam-induced surface nanostructuring. As the irradiation fluence increased, dewetting of the bimetallic films on the Al 2 O 3 substrate was observed, and formation of isolated Ag-Au nanostructures sustained on the substrate were obtained. Next, thermal annealing was performed under high vacuum at 1073 K for 2 hours; a layer of photosensitive Ag-Au alloy nanoballs partially embedded in the Al 2 O 3 substrate was obtained when higher fluence irradiation (> 3.8 × 10 16 cm -2 ) was used. The microstructures of the nanoballs were investigated using a transmission electron microscope, and the nanoballs were found to be single crystals with a FCC structure. In addition, photoabsorption spectra were measured, and localized surface plasmon resonance peaks were observed. With increase in the irradiation fluence, the size of the Ag-Au nanoballs on the substrate decreased, and a blue-shift of the LSPR peaks was observed. Further control of the LSPR frequency over a wide range was achieved by modifying the chemical components, and a red-shift of the LSPR peaks was observed as the Au concentration increased. In summary, ion irradiation is an effective approach toward surface nanostructuring, and the nanocomposites obtained have potential applications in optical devices.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Shift of localized surface plasmon resonance by Ar-ion irradiation of Ag–Au bimetallic films deposited on Al2O3 single crystals

Meng

Shibayama

et al. 2013

Nuclear Instruments and Methods in Physics Research Section B:

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“…Such a system has been used to map an endostatin ePPI network of over 30 proteins [56]. Similarly, SPR imaging (SPRI), also termed SPR microscopy, has potential as a secretomewide screening platform [57][58][59][60][61][62][63]. Technical limitations, however, have hindered full development of SPRI for this application.…”

Section: Spr Imaging Microarraysmentioning

confidence: 99%

Protein microarrays, biosensors, and cell-based methods for secretome-wide extracellular protein–protein interaction mapping

Gonzalez¹

2012

Methods

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Approximately one quarter of all human genes encode proteins that function in the extracellular space or serve to bridge the extracellular and intracellular environments. Physical associations between these secretome proteins serve to regulate a wide range of biological activities and consequently represent important therapeutic targets. Moreover, some extracellular proteins are targeted by pathogens to allow host access or immune evasion. Despite the importance of extracellular protein-protein interactions, our knowledge in this area has remained sparse. Weak affinities and low abundance have often hindered efforts to identify these interactions using traditional methods such as biochemical purification and cDNA library expression cloning. Moreover, current large-scale protein-protein interaction mapping techniques largely under represent extracellular protein-protein interactions. This review highlights emerging biosensor and protein microarray technology, along with more traditional cell-based techniques, that are compatible with secretome-wide screens for extracellular protein-protein interaction discovery. A combination of these approaches will serve to rapidly expand our knowledge of the extracellular protein-protein interactome.

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“…Surface plasmon resonance is a simple technique for studies of biomolecular interactions with huge potential in proteomics (for an overview see Blow, 2009). The readout in SPR is by the use of total internal reflection of a light beam impinging from the back of the sensing surface.…”

Section: Maldi and Ldi Targets For Dual/ Multiple Detectionmentioning

confidence: 99%

Lab‐on‐a‐plate: Extending the functionality of MALDI‐MS and LDI‐MS targets

Urban

Amantonico

Zenobi

2011

Mass Spectrometry Reviews

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We review the literature that describes how (matrix-assisted) laser desorption/ionization (MA)LDI target plates can be used not only as sample supports, but beyond that: as functional parts of analytical protocols that incorporate detection by MALDI-MS or matrix-free LDI-MS. Numerous steps of analytical procedures can be performed directly on the (MA)LDI target plates prior to the ionization of analytes in the ion source of a mass spectrometer. These include homogenization, preconcentration, amplification, purification, extraction, digestion, derivatization, synthesis, separation, detection with complementary techniques, data storage, or other steps. Therefore, we consider it helpful to define the "lab-on-a-plate" as a format for carrying out extensive sample treatment as well as bioassays directly on (MA)LDI target plates. This review introduces the lab-on-plate approach and illustrates it with the aid of relevant examples from the scientific and patent literature.

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Proteins and proteomics: life on the surface

Cited by 40 publications

References 1 publication

Shift of localized surface plasmon resonance by Ar-ion irradiation of Ag–Au bimetallic films deposited on Al2O3 single crystals

Shift of localized surface plasmon resonance by Ar-ion irradiation of Ag–Au bimetallic films deposited on Al2O3 single crystals

Protein microarrays, biosensors, and cell-based methods for secretome-wide extracellular protein–protein interaction mapping

Lab‐on‐a‐plate: Extending the functionality of MALDI‐MS and LDI‐MS targets

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