Proteins and cells on PEG immobilized silicon surfaces

Zhang, Miqin; Desai, Tejal A.; Ferrari, Mauro

doi:10.1016/s0142-9612(98)00026-x

Cited by 386 publications

(283 citation statements)

References 46 publications

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“…Films prepared with TESP generally had more aggregates present on the surface compared to the OTMS films. Water contact angle measurements of the OTMS and TESP films ( Table 2) are consistent with SAMs prepared from octadecyltrichlorosilane and a 4 -6mer oligoethylene glycol grafted silane, respectively (19,22,26). …”

Section: Sam Preparation and Characterizationsupporting

confidence: 67%

“…Grafted polymers and self-assembled monolayers are methods used to introduce functionality to surfaces, as well as establishing a packing density on the surface and specific orientation of the functionality. Polyethylene glycol (PEG), or polyethyleneoxide (PEO), functionalized surfaces are examples of hydrophilic coatings that offer excellent resistance to protein and cellular adhesion as both grafted polymer and SAM films (19,20,21,22,23,24). In contrast, hydrophobic surfaces, such as octadecyltrichlorosilane SAMs, tend to adsorb proteins as a monolayer thick biofilm (25,26,27).…”

Section: Introductionmentioning

confidence: 99%

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Protein Adhesion on SAM Coated Semiconductor Wafers: Hydrophobic versus Hydrophilic Surfaces

Follstaedt¹,

Cheung²,

Gourley³

et al. 2000

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The interface of biology and semiconductor materials has become an important topic of interest as researchers are merging living organisms with microelectromechanical systems (MEMS) in the pursuit of new microfabricated biomedical devices. Biological cells interface with the semiconductor surface through a host of interactions that are mediated by protein adhesion and film formation. To better understand this interface, we have conducted protein adsorption studies of a model system using bovine serum albumin (BSA) and compared it to the adsorption from a complex protein mixture of cell growth serum on uncoated and self-assembled monolayer (SAM) coated silicon wafers. Several characterization techniques -AFM, ellipsometry, water contact angle, and fluorescence microscopy -were used to evaluate the protein-adsorbed layer. An uncoated silicon surface was most attractive to proteins, forming a 70 Å thick film from a solution of BSA at a concentration comparable to that in cell growth serum. Coating with the hydrophobic octadecyltrimethoxysilane (OTMS) SAM reduced protein adhesion by ~15%. In contrast, a hydrophilic N-(triethoxysilylpropyl)-O-polyethyleneoxide urethane (TESP) SAM inhibited protein adhesion by greater than 50%. Protein adhesion studies with cell growth sera containing complex mixtures of proteins paralleled the BSA adsorption studies, clearly identifying the TESP coated surface as a promising biocompatible coating.4

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Section: Sam Preparation and Characterizationsupporting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Protein Adhesion on SAM Coated Semiconductor Wafers: Hydrophobic versus Hydrophilic Surfaces

Follstaedt¹,

Cheung²,

Gourley³

et al. 2000

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“…However, improvements are required in many areas, such as prevention of non-specific binding [302][303][304][305][306], sensitivity [307][308][309][310][311][312][313][314][315], site-specific conjugation [316][317][318], bioactivity [319][320][321][322], multiplexing [309,323], and reusability [324][325][326]. Some of these problems can be addressed by using different chemical surface modification approaches.…”

Section: Biosensing-detectionmentioning

confidence: 99%

Peptide-based biopolymers in biomedicine and biotechnology

Chow

Nunalee

Lim

et al. 2008

Materials Science and Engineering: R: Reports

272

227

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Peptides are emerging as a new class of biomaterials due to their unique chemical, physical, and biological properties. The development of peptide-based biomaterials is driven by the convergence of protein engineering and macromolecular self-assembly. This review covers the basic principles, applications, and prospects of peptide-based biomaterials. We focus on both chemically synthesized and genetically encoded peptides, including poly-amino acids, elastin-like polypeptides, silk-like polymers and other biopolymers based on repetitive peptide motifs. Applications of these engineered biomolecules in protein purification, controlled drug delivery, tissue engineering, and biosurface engineering are discussed.

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“…In order for the surface modification to inhibit cell adhesion, the substrate used must be through a cleaning protocol first by immersed in a piranha solution [16,1,17,18,15,26,19]. The silicon substrate need to be cleaned to get rid of the contaminates before the substrate was ready for the coating process.…”

Section: Principles Of Surface Functionalizationmentioning

confidence: 99%

The study of non-fouling and non-specific cellular binding on functionalized surface for mammalian cell identification and manipulation

Zainudin¹,

Hambali²,

Wahid³

et al. 2017

AIP Conference Proceedings

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Signal characteristics by optimization the output coupling ratio of multi-wavelength Brillouin fiber laser incorporating fiber Bragg grating in a ring cavity technique AIP Conference Proceedings 1835, 020002 (2017) Abstract.Surface functionalization has emerged as a powerful tool for mapping limitless surface-cell membrane interaction in diverse biomolecular applications. Inhibition of non-specific biomolecular and cellular adhesion to solid surfaces is critical in improving the performance of some biomedical devices, particularly for in vitro bioassays. Some factors have to be paid particular attention in determining the right surface modification which are the types of surface, the methods and chemical solution that being used during the experimentation and also tools for analyzing the results. Improved surface functionalization technologies that provide better non-fouling performance in conjunction with specific attachment chemistries are sought for these applications. Hence, this paper serves as a review for multiple surface treatment methods including PEG grafting, adsorptive chemistries, self-assembled monolayers (SAMs) and plasma treatments.

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Proteins and cells on PEG immobilized silicon surfaces

Cited by 386 publications

References 46 publications

Protein Adhesion on SAM Coated Semiconductor Wafers: Hydrophobic versus Hydrophilic Surfaces

Protein Adhesion on SAM Coated Semiconductor Wafers: Hydrophobic versus Hydrophilic Surfaces

Peptide-based biopolymers in biomedicine and biotechnology

The study of non-fouling and non-specific cellular binding on functionalized surface for mammalian cell identification and manipulation

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