Proteins and Catalase Isoenzymes from Fusarium Solani and their Taxonomic Significance

Abstract

SummaryProteins 'and catalase isoenzymes from saprophytic and parasitic forms of FUBarium Bolani were separated electrophoretically in starch gels. Protein patterns of an isolate of F. Bolani f. pisi showed little response to altered cultural conditions but catalase patterns altered with culture age, illumination of culture, number of conidia in the inoculum, and portion of growth harvested. Under optimum conditions a maximum of four catalase bands was detected. Evidence is presented that hyphae and spores of this isolate have different catalase patterns and that these patterns are influenced by light intensity.Under conditions optimum for the above isolate of F. Bolani f. pisi, culturally and pathogenically distinct isolates of F. Bolani produced essentially identical protein patterns but markedly different catalase patterns. The taxonomic implications of this are discussed.

Peroxidase, Polyphenoloxidase and Ribonuclease in Tobacco Necrosis Virus Infected or Mannitol Osmotically-stressed Cowpea and Cucumber Tissue.

“…Zones of enzyme activity (PO isozymes) were visualised as yellowish-brown bands. The gels were scanned immediately in a densitometer at 520 nm (HALL 1967), and Rf values of bands of interest were calculated as above.…”

Genetic Analysis in Wood‐Decaying Fungi I. Genetic Variation and Evidence for Allopatric Speciation in Pleurotus ostreatus using Phenoloxidase Zymograms and Morphological Criteria

“…Blaich (1977) stressed the advantages that the employment of specifically stained enzyme spectra, such as those of the phenoloxidases (laccases), esterases, and aminopeptidases, has over spectra of uniformly stained proteins. The bulk of the fungus enzyme-chemotaxonomic literature is concerned with the phyco-, asco-and deuteromycetes (Nealson and Garher 1967, Hall 1967, Meyer and Renard 1969, Stibes 1970, Nasuno 1972, Stout and Shaw 1973, Bucher 1974. Setvaraj and Meyer 1974, Abbott and Holland 1975.…”

The Role of Phenolic Compounds in the Resistance of Tomato Cultivars to Verticillium albo‐atrum

“…(iii) Staining and estimating of gels, (a) Peroxidase. The gels were stained using pyrogallol and hydrogen peroxide (HALL 1967). Peroxidase bands appear brown against a yellow background.…”