Proteinergic profiles in cerebrospinal fluid from alcoholic subjects

Abstract

Minute amounts of cerebrospinal fluid samples from alcoholics were subjected to separation by HPLC-molecular sieving, combined with multispectral UV analysis of the acquired data. A significant difference in the protein/polypeptide pattern within the molecular weight range of 7-10 kDa has been observed between samples, taken directly after detoxification and 2 weeks later. Spectral analysis of the results suggests that the components are of peptidergic nature. On the other hand, albumin content did not differ significantly, suggesting that the blood-brain barrier was not affected. An enzyme marker, dynorphin converting enzyme, remained unchanged in both groups.

Chapter 5 Size-exclusion chromatography

“…HPSEC, combined with multispectral detection by a photodiode-array (PDA) UV detector was applied to the analysis of proteins and peptides in human cerebrospinal fluid (CSF) [125] and to the characterization of proteinergic profiles in the CSF of alcoholics [ 126]. Molecular components of the CSF were identified, and their purity was tested.…”