Proteinadsorption behavior in batch and competitive conditions with nanoparticle surface imprinting

Sankarakumar, Niranjani; Tong, Yen Wah

doi:10.1039/c2ra21830e

Cited by 17 publications

(16 citation statements)

References 38 publications

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“…Similar trends have been attained for previous SDVGs [54]. These similar kinetic fashions are possibly due to a saturation of binding sites of biomaterials [55][56][57] or the combination effects of hydrophilicity, size, charge, stability, rigidity, texture, and availability of binding sites of the protein-biomaterial system [58].…”

Section: Discussionsupporting

confidence: 76%

Synthesis and characterization of polycaprolactone urethane hollow fiber membranes as small diameter vascular grafts

Mercado-Pagán

Stahl

Ramseier

et al. 2016

Materials Science and Engineering: C

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The design of bioresorbable synthetic small diameter (<6mm) vascular grafts (SDVGs) capable of sustaining long-term patency and endothelialization is a daunting challenge in vascular tissue engineering. Here, we synthesized a family of biocompatible and biodegradable polycaprolactone (PCL) urethane macromers to fabricate hollow fiber membranes (HFMs) as SDVG candidates, and characterized their mechanical properties, degradability, hemocompatibility, and endothelial development. The HFMs had smooth surfaces and porous internal structures. Their tensile stiffness ranged from 0.09 to 0.11N/mm and their maximum tensile force from 0.86 to 1.03N, with minimum failure strains of approximately 130%. Permeability varied from 1 to 14×10(-6)cm/s, burst pressures from 1158 to 1468mmHg, and compliance from 0.52 to 1.48%/100mmHg. The suture retention forces ranged from 0.55 to 0.81N. HFMs had slow degradation profiles, with 15 to 30% degradation after 8weeks. Human endothelial cells proliferated well on the HFMs, creating stable cell layer coverage. Hemocompatibility studies demonstrated low hemolysis (<2%), platelet activation, and protein adsorption. There were no significant differences in the hemocompatibility of HFMs in the absence and presence of endothelial layers. These encouraging results suggest great promise of our newly developed materials and biodegradable elastomeric HFMs as SDVG candidates.

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Section: Discussionsupporting

confidence: 76%

Synthesis and characterization of polycaprolactone urethane hollow fiber membranes as small diameter vascular grafts

Mercado-Pagán

Stahl

Ramseier

et al. 2016

Materials Science and Engineering: C

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“…These kinetic patterns on different surfaces have been seen and reported frequently in the literature for proteins [36][37][38] and platelets. 39 Potentially, modification of surface Mercado-Pagán et al…”

Section: Discussionmentioning

confidence: 76%

Hemocompatibility evaluation of small elastomeric hollow fiber membranes as vascular substitutes

2014

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One of the main challenges for clinical implementation of small diameter vascular grafts (SDVGs) is their limited hemocompatibility. Important design specifications for such grafts include features that minimize the long-term risks of restenosis, fouling, and thrombus formation. In our lab, we have developed elastomeric hollow fiber membranes (HFMs), using a phase inversion method, as candidates for SDVGs. Here, we present our results for in vitro hemocompatibility testing of our HFM under flow and static conditions. Our results showed that the polymer-based HFMs do not damage the integrity of human red blood cells (RBCs) as shown by their low hemolytic extent (less than 2%). When analyzed for blood cell lysis using lactate dehydrogenase (LDH) activity as an indicator, no significant differences were observed between blood exposed to our HFMs and uncoagulated blood. Analysis of protein adsorption showed a low concentration of proteins deposited on the surfaces of HFM after 24 h. Platelet adhesion profiles using human platelet-rich plasma (PRP) showed that a low level of platelets adhered to the HFMs after 24 h, indicating minimal thrombotic potential. Under the majority of conditions, no significant differences were observed between medical-grade polymers and our HFMs. Eventual optimization of hemocompatible elastomeric HFM vessel grafts could lead to improved tissue vascularization as well as vascularized, tissue-engineered scaffolds for organ repair.

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“…Step 4: rebinding through recognition by functional pores. Turner et al, 2006;Bossi et al, 2007;Chen et al, 2008;Uysal et al, 2008;Hua et al, 2009;Whitcombe et al, 2011;Sankarakumar and Tong, 2013;, and in the succeeding texts, we formalize their effect. Functional group distribution was also suggested as a contributor to protein selectivity (Kimhi and Bianco-Peled, 2007;Gai et al, 2010;Levi and Srebnik, 2011;Zayats et al, 2011;Lan et al, 2014).…”

Section: Methodsmentioning

confidence: 99%

“…AR and SR were estimated, as described later. MW (Pang et al ., ; Odabaşi et al ., ; Çimen et al ., ; Pan et al ., ) and IEP (Pan et al ., ; El‐Sharif et al ., ; Zhang et al ., ) have already been suggested as possible explanations for observed PIP selectivity in an ad hoc manner in the literature (Bossi et al ., ; Turner et al ., ; Bossi et al ., ; Chen et al ., ; Uysal et al ., ; Hua et al ., ; Whitcombe et al ., ; Sankarakumar and Tong, ; Li et al ., 2014), and in the succeeding texts, we formalize their effect. Functional group distribution was also suggested as a contributor to protein selectivity (Kimhi and Bianco‐Peled, ; Gai et al ., ; Levi and Srebnik, ; Zayats et al ., ; Lan et al ., ).…”

Section: Methodsmentioning

confidence: 99%

Comparison of descriptors for predicting selectivity of protein‐imprinted polymers

Raim

Zadok

Srebnik

2016

J of Molecular Recognition

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Molecular imprinting is a technique that is used to create artificial receptors by the formation of a polymer network around a template molecule, creating a molecularly imprinted polymer. These artificial receptors may be used in applications that require molecular recognition, such as enantioseparations, biosensors, artificial catalysis, drug delivery and others. Small molecules, such as drugs, have been imprinted with high efficiency and, combined with the low cost of preparation, molecularly imprinted polymers have acquired commercial usage. While attempts at imprinting proteins have been significantly less successful, the great potential of protein-imprinted polymers (PIPs) in medicine and industry attracted much research. Multifunctionality, conformational flexibility, large size of the proteins, and aqueous polymerization environment are some of the obstacles faced by protein imprinting. We explore the relation between PIP selectivity and the properties of the template and competitor proteins. A comprehensive statistical analysis of published studies reveals a statistically significant correlation between four protein descriptors and the corresponding selectivity of PIPs. Namely, a PIP will generally be more selective against large competitor proteins with a smooth surface, whose isoelectric point and aspect ratio are significantly different than those of the template protein. The size of the protein, as measured by its molecular weight, appears to be independent of the template protein characteristics. Copyright © 2016 John Wiley & Sons, Ltd.

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Proteinadsorption behavior in batch and competitive conditions with nanoparticle surface imprinting

Cited by 17 publications

References 38 publications

Synthesis and characterization of polycaprolactone urethane hollow fiber membranes as small diameter vascular grafts

Synthesis and characterization of polycaprolactone urethane hollow fiber membranes as small diameter vascular grafts

Hemocompatibility evaluation of small elastomeric hollow fiber membranes as vascular substitutes

Comparison of descriptors for predicting selectivity of protein‐imprinted polymers

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