Proteinaceous Nano container Encapsulate Polycyclic Aromatic Hydrocarbons

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are toxic, mutagenic and among the most damaging chemical compounds with regard to living organisms. Because of their persistence and wide distribution removal from the environment is an important challenge. Here we report a new Nano container matrix based on the deep sea archaea-derived RHCC-Nanotube (RHCC-NT), which rapidly and preferentially binds low molecular weight PAHs. Under controlled-laboratory conditions and using fluorescence spectroscopy in combination with X-ray crystallography and MD simulations, we quantified the real-time binding of low molecular weight PAHs (2–4 rings) to our substrate. Binding coefficients ranged from 5.4 ± 1.6 (fluorene) to 32 ± 7.0 μM (acenaphthylene) and a binding capacity of 85 pmoles PAH per mg RHCC-NT, or 2.12 μmoles in a standard 25 mg sampler. The uptake rate of pyrene was calculated to be 1.59 nmol/hr∙mol RHCC-NT (at 10 C). Our results clearly show that RHCC-NT is uniquely suited as a monitoring matrix for low molecular weight PAHs.

Mannose‐Decorated Composite Peptide Hydrogel with Thixotropic and Syneresis Properties and its Application in Treatment of Leishmaniasis

“…Thereafter, we moved on to optimize the gelation kinetics of Gel‐A and Gel‐B. The gelation kinetics of 1 wt.% (15 mM) pre‐gelation solutions of Gel‐A (Sol‐A) and Gel‐B (Sol‐B) was monitored through time‐dependent quenching of naphthalene fluorescence detected at 337 nm [20] . From the obtained curve, it is deciphered that ‘Sol ‐ A’ forms hydrogel within 5 minutes.…”

“…The gelation kinetics of 1 wt.% (15 mM) pregelation solutions of Gel-A (Sol-A) and Gel-B (Sol-B) was monitored through time-dependent quenching of naphthalene fluorescence detected at 337 nm. [20] From the obtained curve, it is deciphered that 'Sol-A' forms hydrogel within 5 minutes. 'Sol-B' followed a slower path and formed gel after 10 minutes (Figure S1A).…”

Automotive Diesel Fuel Internal Stability Testing with the Use of UV and Temperature as Degradation Factors

“…Pyrene can act as an efficient fluorescent probe of polar solutions. The changes in the polarity of the immediate environment of the pyrene can result in the fluorescence spectrum changes of intensity or shift wavelength of peak [ 18 , 19 ]. Fluorescent sensitivity of pyrene to lipids was reported, with peaks of emissions observed at 375 nm, 400 nm, and a hill at 460 nm [ 20 ].…”

Molecular dynamics simulations of ortho-carborane nano-diamond storage within the nonpolar channel cavities of a right-handed coiled-coil tetrabrachion nanotube

“…X-ray diffraction measurements [5] performed on a polypeptide chain fragment of tetrabrachion show that the stalk is a tetrameric right-handed coiled-coil composed of four strands oriented in parallel in a right-handed fashion, forming a nanotube with a central channel and four large non-polar cavities which contain water clusters in the native conformation. The has been shown [5] to be stable at high salt concentrations, temperatures of over 100 ˚C, high pressures and extreme ranges of pH, and several studies have demonstrated that the hydrophobic cavities are able to uptake and store water clusters [5] , elemental sulfur crowns [8] , [9] , atomic metals clusters [10] , and polycyclic hydrocarbons [11] .…”