Proteinaceous inhibitors of microbial xylanases

Gusakov, Alexander V.

doi:10.1134/s0006297910100019

Cited by 26 publications

(18 citation statements)

References 83 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Plants have evolved inhibitors of pathogenic microbial enzymes as defense components. Numerous inhibitors of microbial enzymes have been identified and characterized from plants [14–16]. A .…”

Section: Introductionmentioning

confidence: 99%

Aspergillus ficuum phytase activity is inhibited by cereal grain components

et al. 2017

View full text Add to dashboard Cite

In the current study, we report for the first time that grain components of barley, rice, wheat and maize can inhibit the activity of Aspergillus ficuum phytase. The phytase inhibition is dose dependent and varies significantly between cereal species, between cultivars of barley and cultivars of wheat and between Fusarium graminearum infected and non-infected wheat grains. The highest endpoint level of phytase activity inhibition was 90%, observed with grain protein extracts (GPE) from F. graminearum infected wheat. Wheat GPE from grains infected with F. graminearum inhibits phytase activity significantly more than GPE from non-infected grains. For four barley cultivars studied, the IC50 value ranged from 0.978 ± 0.271 to 3.616 ± 0.087 mg×ml-1. For two non-infected wheat cultivars investigated, the IC50 values were varying from 2.478 ± 0.114 to 3.038 ± 0.097 mg×ml-1. The maize and rice cultivars tested gaveIC50 values on 0.983 ± 0.205 and 1.972 ± 0.019 mg×ml-1, respectively. After purifying the inhibitor from barley grains via Superdex G200, an approximately 30–35 kDa protein was identified. No clear trend for the mechanism of inhibition could be identified via Michaelis-Menten kinetics and Lineweaver-Burk plots. However, testing of the purified phytase inhibitor together with the A. ficuum phytase and the specific protease inhibitors pepstatin A, E64, EDTA and PMSF revealed that pepstatin A repealed the phytase inhibition. This indicates that the observed inhibition of A. ficuum phytase by cereal grain extracts is caused by protease activity of the aspartic proteinase type.

show abstract

Section: Introductionmentioning

confidence: 99%

Aspergillus ficuum phytase activity is inhibited by cereal grain components

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Xylanase inhibitor proteins (XIP) are considered potential defense molecules, which prevent cell wall degradation by hydrolytic fungal enzymes (1297). The presence of thaumatin-like xylanase inhibitors (TLX) and chitinase-like xylanase inhibitor proteins was recently reported in plants (1296).…”

Section: Proteins With Known Effector Motifsmentioning

confidence: 99%

“…TLPs are induced in plants in response to various biotic and abiotic stresses and have been thought to play a role in plant defense systems, particularly in systemically acquired resistance (1295). TLPs are known to accumulate in plants in response to infection by a pathogen, and some are known to have antifungal properties (1294,(1296)(1297)(1298)(1299). Some TLPs hydrolyze crude fungal walls, and one barley TL enzyme even lyses fungal spores (1295).…”

Section: Proteins With Known Effector Motifsmentioning

confidence: 99%

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Schmoll

Dattenböck

Carreras-Villaseñor

et al. 2016

Microbiol Mol Biol Rev

163

195

View full text Add to dashboard Cite

SUMMARYThe genusTrichodermacontains fungi with high relevance for humans, with applications in enzyme production for plant cell wall degradation and use in biocontrol. Here, we provide a broad, comprehensive overview of the genomic content of these species for “hot topic” research aspects, including CAZymes, transport, transcription factors, and development, along with a detailed analysis and annotation of less-studied topics, such as signal transduction, genome integrity, chromatin, photobiology, or lipid, sulfur, and nitrogen metabolism inT. reesei,T. atroviride, andT. virens, and we open up new perspectives to those topics discussed previously. In total, we covered more than 2,000 of the predicted 9,000 to 11,000 genes of eachTrichodermaspecies discussed, which is >20% of the respective gene content. Additionally, we considered available transcriptome data for the annotated genes. Highlights of our analyses include overall carbohydrate cleavage preferences due to the different genomic contents and regulation of the respective genes. We found light regulation of many sulfur metabolic genes. Additionally, a new Golgi 1,2-mannosidase likely involved inN-linked glycosylation was detected, as were indications for the ability ofTrichodermaspp. to generate hybrid galactose-containingN-linked glycans. The genomic inventory of effector proteins revealed numerous compounds unique toTrichoderma, and these warrant further investigation. We found interesting expansions in theTrichodermagenus in several signaling pathways, such as G-protein-coupled receptors, RAS GTPases, and casein kinases. A particularly interesting feature absolutely unique toT. atrovirideis the duplication of the alternative sulfur amino acid synthesis pathway.

show abstract

“…Organizational-and-technological reliability (OTR) is the ability, the existing probability of the designed decisions relating to costs planning of resources including labour, to determination of duration of construction of a separate object or complex in general, to be executed within the established concept of the construction project and with the set level of quality [1][2][3][4][5]. Numerous works of the famous scientists [1,3,[5][6][7][8][9][10][11][12][13][14] are devoted to the matter of increase in OTR of construction system (in general and its components of elements), however this question remains relevant and today. In works [3][4][5][6] it is noted that the construction process always has probabilistic character, since work flow is influenced by various random factors having the diverse nature and various consequences all the time.…”

Section: Introductionmentioning

confidence: 99%

“…Numerous works of the famous scientists [1,3,[5][6][7][8][9][10][11][12][13][14] are devoted to the matter of increase in OTR of construction system (in general and its components of elements), however this question remains relevant and today. In works [3][4][5][6] it is noted that the construction process always has probabilistic character, since work flow is influenced by various random factors having the diverse nature and various consequences all the time.…”

Section: Introductionmentioning

confidence: 99%

Scheduling on the basis of the research of dependences among the construction process parameters

Романович

Ermakov

Mukhamedzhanova

2017

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

Abstract. The dependences among the construction process parameters are investigated in the article: average integrated value of qualification of the shift, number of workers per shift and average daily amount of completed work on the basis of correlation coefficient are considered. Basic data for the research of dependences among the above-stated parameters have been collected during the construction of two standard objects A and B (monolithic houses), in four months of construction (October, November, December, January). Kobb-Douglas production function has proved the values of coefficients of correlation close to 1. Function is simple to be used and is ideal for the description of the considered dependences. The development function, describing communication among the considered parameters of the construction process, is developed. The function of the development gives the chance to select optimum quantitative and qualitative (qualification) structure of the brigade link for the work during the next period of time, according to a preset value of amount of works. Function of the optimized amounts of works, which reflects interrelation of key parameters of construction process, is developed. Values of function of the optimized amounts of works should be used as the average standard for scheduling of the storming periods of construction. IntroductionConstruction process is the difficult system, which values of parameters constantly changes in time and depend on a huge number of factors. Organizational-and-technological reliability of construction process (OTRCP) is directly connected with the decisions, made when scheduling construction. Therefore scheduling always needs special attention. Matters of the increase in OTRCP of construction always were relevant. The necessary OTRCP level is provided with the factors, connected with organizational, technology, technical, economic solutions. Therefore, consideration of the applied methods of management of such factors is also very important.The increase in efficiency of processing of statistical information on parameters of construction process is an important task for establishment of dependences between processes of construction system and further forecasting of her behavior. Important feature of the parameters of construction process investigated in work is shift of these parameters in time. The models describing the temporary ranks formed by values of the studied parameters represent difficult dependences. For identification of such dependences it is necessary to use such mathematical apparatus, which could reveal the moments of emergence of such dependences and their frequency with the maximum reliability. It is especially important to reveal minor changes in the parameters of the construction system, which sometimes can lead to critical deviations of parameters of construction from preset values, for example, the increase in

show abstract

Proteinaceous inhibitors of microbial xylanases

Cited by 26 publications

References 83 publications

Aspergillus ficuum phytase activity is inhibited by cereal grain components

Aspergillus ficuum phytase activity is inhibited by cereal grain components

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Scheduling on the basis of the research of dependences among the construction process parameters

Contact Info

Product

Resources

About