2022
DOI: 10.1002/mbo3.1264
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Protein with negative surface charge distribution, Bnr1, shows characteristics of a DNA‐mimic protein and may be involved in the adaptation of Burkholderia cenocepacia

Abstract: Adaptation of opportunistic pathogens to their host environment requires reprogramming of a vast array of genes to facilitate survival in the host. Burkholderia cenocepacia, a Gram‐negative bacterium with a large genome of ∼8 Mb that colonizes environmental niches, is exquisitely adaptable to the hypoxic environment of the cystic fibrosis lung and survives in macrophages. We previously identified an immunoreactive acidic protein encoded on replicon 3, BCAS0292. Deletion of the BCAS0292 gene significantly alter… Show more

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Cited by 4 publications
(5 citation statements)
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“…MAX02 (AVR-PikC), MAX58, MAX62 (AVR-Pib), MAX80). It is well established that positively charged regions in proteins are important for interaction with negatively charged macromolecules, such as nucleic acids and lipopolysaccharides [38], whereas negatively charged protein surfaces can be involved in membrane attachment or DNA mimicking functions [39][40][41]. In MAX47, we noticed that its unstructured N-terminal extension is rich in aspartic residues, suggesting that it could make transient interactions with the positively charged MAX core in the absence of its cellular target.…”
Section: Max Domains Exhibit Highly Variegated Surface Propertiesmentioning
confidence: 83%
“…MAX02 (AVR-PikC), MAX58, MAX62 (AVR-Pib), MAX80). It is well established that positively charged regions in proteins are important for interaction with negatively charged macromolecules, such as nucleic acids and lipopolysaccharides [38], whereas negatively charged protein surfaces can be involved in membrane attachment or DNA mimicking functions [39][40][41]. In MAX47, we noticed that its unstructured N-terminal extension is rich in aspartic residues, suggesting that it could make transient interactions with the positively charged MAX core in the absence of its cellular target.…”
Section: Max Domains Exhibit Highly Variegated Surface Propertiesmentioning
confidence: 83%
“…We previously reported that USP76 and USP92, showed up to 6‐ and 16‐fold increased abundance, respectively, from early to late infection in two sets of sequential isolates and were selected for further investigation (Cullen et al, 2018 ). Moreover, we showed that the abundance of these was reduced by 1.66‐ and 4.5‐fold when BCAS0292, which encodes the DNA mimic protein Bnr1, was deleted (Dennehy et al, 2022 ).…”
Section: Resultsmentioning
confidence: 99%
“…These late chronic infection isolates also showed increased attachment to CF lung epithelial cells relative to their respective early isolates (Cullen et al, 2017 ). Importantly, all six USPs encoded on the lxa locus showed increased protein abundance with the time of colonization (Cullen et al, 2018 ) and five of which were regulated by the DNA mimic protein, Bnr1 (Dennehy et al, 2022 ). Among these lxa ‐encoded usp genes, BCAM0276 encodes a UspA family stress protein which consistently showed increased abundance in the later isolates from two chronically colonized patients and was associated with increased gene expression (Cullen et al, 2018 ).…”
Section: Introductionmentioning
confidence: 99%
“…(AVR-Pib), MAX80). It is well known that positively charged regions in proteins are important for interaction with negatively charged macromolecules, such as nucleic acids and lipopolysaccharides [32], whereas negatively charged protein surfaces can be involved in membrane attachment or DNA mimicking functions [33][34][35]. In MAX47, we noticed that its unstructured N-terminal extension is rich in Asp residues, suggesting that it could make transient interactions with the positively charged MAX core in the absence of its cellular target.…”
Section: Max Domains Exhibit Very Variegated Surface Propertiesmentioning
confidence: 88%
“…However, size differences or structural elements adding to their common fold can hamper such analysis. We therefore performed a detailed comparative analysis of the surface properties of the bona fide MAX effectors by focusing on the MAX core domains extracted from 49 AF_MAX models in which no structured regions interacted with the central core (S7a regions in proteins are important for interaction with negatively charged macromolecules, such as nucleic acids and lipopolysaccharides [36], whereas negatively charged protein surfaces can be involved in membrane attachment or DNA mimicking functions [37][38][39]. In MAX47, we noticed that its unstructured Nterminal extension is rich in aspartic residues, suggesting that it could make transient interactions with the positively charged MAX core in the absence of its cellular target.…”
Section: Max Domains Exhibit Highly Variegated Surface Propertiesmentioning
confidence: 99%