Protein Uptake in the Oocytes of the Cecropia Moth

Abstract

The formation of yolk spheres in the oocyte of the cecropia moth, Hyalophora cecropia (L.), is known immunologically to result largely from uptake of a sex-limited blood protein.Recent electron microscope analyses of insect and other animal oocytes have demonstrated fine structural configurations consistent with uptake of proteins by pinocytosis. An electron microscope analysis of the cecropia ovary confirms the presence of similar structural modifications. With the exception of two apparently amorphous layers, the basement lamella on the outer surface of the follicular epithelium and the vitelline membrane on the inner, there is free access of blood to the oocyte surface between follicle cells. Dense material is found in the interfollicular cell space and adsorbed to the outer surface of the much folded oocyte membrane. Pits in the oocyte membrane and vesicles immediately under it are lined with the same dense material not unlike the yolk spheres in appearance. Introduction of ferritin into the blood of a developing cecropia moth and its localization adsorbed to the surface of the oocyte, and within the vesicles and yolk spheres of the oocyte cortex, is experimental evidence that the structural modifications of the oocyte cortex represent stages in the pinocytosis of blood proteins which arrive at the oocyte surface largely by an intercellular route. Small tubules attached to the yolk spheres are provisionally interpreted as a manifestation of oocyte-synthesized protein being contributed to the yolk spheres.

Electron Microscopic Studies on the Oogenesis of Dragonfly and Cricket With Special Reference to the Panoistic Ovaries

“…Micropinocytosis during yolk formation in insect oocytes has been observed in numerous species; insects with panoistic type ovaries, such as Lepisma (CONE and SCALZI, 1967), Gryllus (FAVARD-SER~NO, 1966), Periplaneta (ANDERSON, 1964;MILLS, GREENSLADE and Coucn, 1966) and Aeschna , and those with polytrophic ovaries, such as Drosophila (KING et al, 1958;OKADA and WADDINGTON, 1959), Aedes (ROTH and PORTER, 1964), Hyalophora (KING and AGGARWAL, 1965;STAY, 1965;ANDERSON and TELFER, 1969), Panorpa (RAMAMURTY, 1964;MATSUZAKI, 1967), Bombus (HOPKINS and KING, 1966) and Parastenopsyche (MATSUZAKI, 1967), and those with telotrophic ones such as Oncopeltus (KESSEL and BERMS, 1962), Epilachna and Bethrogoniu (MATSUZAKI, 1967). Pinocytotic incorporation of the proteid yolk precursors may well be correlated to the vitellogenesis of the growing oocyte in panoistic ovarioles, in which no specialized nurse cells have developed, but it is not limited to the vitellogenesis in panoistic oocytes.…”

Ovarian Development of House Fly (Musca domestica L.) (Diptera: Muscidae)

“…The presence of yolk granules at the apex of follicular cells in present evidence for the intracellular pathway for protein accumulation inside the oocyte. Investigators like [42,43] have also reported that the proteins from the hemolymph are transported through the intracellular spaces of the epithelium in to the developing oocytes during vitellogenesis of the insects. It is opined that the enlargement of the inter follicular channels is one of the vitellogenic events in the follicular cells at the one set of vitellogenesis.…”

Estudo de mecanismos envolvidos na incorporação de proteínas durante a ovogênese em Anopheles

“…Na primeira, exógena, as proteínas são sintetizadas no corpo gorduroso e são transportadas peía hemolinfa até o ovócíto, como demonstrado em vários insetos: Aedes (Roth & Porter, 1964;Hagedorn et ai., 1973), Periplaneta (Anderson, 1964), Panorpa (Bier & Ramamurty, 1964), Platysamia cecropia (Telfer, 1954), Lygeus kalmii (Kessel & Beams, 1962), Hyalophora cecropia (Stay, 1965), Schistocerca (Hill, 1962), LepismachilIs(BÍtsch,…”

“…Em Anopheles nãoforamob servados espaços tio proeminentes como em Aedes aegypti (Roth & Porter,13&k)e Hyalopho ra cecropia (Stay, 1965), mas isso nao significa a falta de acesso da vitelogenina da he molinfa. Um fato que também corrobora a hipótese da passagem de material externo para o ovócito é que esses espaços não foram encontrados entre as células foliculares em con_ tato com as células nutridoras (Figura 1).…”