Protein trapping of silicananoparticles

Ang, Joo Chuan; Lin, Jhih-Min; Yaron, Peter N.; White, John W.

doi:10.1039/b919256e

Cited by 21 publications

(19 citation statements)

References 15 publications

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“…One exception is the pioneering neutron reflectivity study of the structure of a mixed layer of b-casein þ silica nanoparticles (Ang, Lin, Yaron, & White, 2010). Based on time-dependent measurements at the airewater interface, these investigators concluded that, because the hydrophilic silica particles were not themselves surface-active, they became gradually incorporated into the adsorbed layer through associative interaction with free bcasein molecules.…”

Section: Synergism In Mixed Systems Of Particles and Polymersmentioning

confidence: 98%

Exploring the frontiers of colloidal behaviour where polymers and particles meet

Dickinson

2016

Food Hydrocolloids

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Section: Synergism In Mixed Systems Of Particles and Polymersmentioning

confidence: 98%

Exploring the frontiers of colloidal behaviour where polymers and particles meet

Dickinson

2016

Food Hydrocolloids

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“…Recently, Kendall et al [23] studied the aggregation behavior of nanoparticles induced by fibrinogen, in order to assess the toxicity of the particles in relation to the proteins. In a different context, Ang et al [33] studied the interaction of silica nanoparticles with ß-casein at air-water interface and its effect on the surface activity of the silica-protein complexes. Bridging attraction and heteroaggregation of silica nanoparticles was also found under the influence of adsorbed polyethylene oxide [34] and of thermo-responsive polymer chains [35].…”

Section: Introductionmentioning

confidence: 99%

Aggregation of Silica Nanoparticles Directed by Adsorption of Lysozyme

Bharti

2014

Adsorption, Aggregation and Structure Formation in Systems of Charged Particles

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Interaction of biomolecules with nanoscale materials has attracted much attention in recent years, triggered by promising new developments in pharmaceutical and biomedical technology. Nanoparticles are used as carriers in biomolecular transport and drug delivery, regenerative medicine, biosensing, imaging and related applications [1][2][3][4]. Immobilization of proteins on nanoscale materials is usually based on adsorption [5][6][7][8], entrapment [9][10][11], or covalent binding [12,13]. Adsorption from aqueous media onto hydrophilic surfaces can in many cases be controlled by pH and ionic strength and in this way allows controlled binding and release of the protein molecules. Fundamentals of the adsorption equilibrium and kinetics of proteins have been studied at well-defined flat model surfaces, using surface-sensitive spectroscopic techniques [14,15], ellipsometry or optical reflectometry [16,17], and neutron reflectometry [18]. Adsorption onto nanoscale surfaces introduces a higher level of complexity, as their high surface curvature can influence the adsorption as well as the structure and biological activity of the protein [5,6,[19][20][21][22]. On the other hand, the growing importance of nanosize particles in everyday life makes studies of their interaction with biomolecules a matter of high importance [23][24][25].Aqueous dispersions of oxide nanoparticles containing globular proteins also represent interesting model systems for studying fundamentals of colloidal interaction. Globular proteins such as lysozyme exhibit the major characteristics of colloids that interact via a short-range attractive potential superimposed with repulsive electrostatic interactions [26]. Under certain conditions the short-range attraction may cause the formation of small clusters which are stabilized against further growth by electrostatic repulsion. However, the physical nature of these protein clusters is still not fully understood and remains an active field of current research [27][28][29][30]. Oxide particles such as silica are prototypical for colloid systems interacting by van der Waals

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“…Figure 2 shows the real space scattering length density distribution at each time of measurement. The distribution corresponds to that expected [4] for a mixture of weaker scattering protein and strongly scattering nanoparticle, with protein above and below the mixed nanoparticle protein region. The Figure shows the quick formation of the film and its stability with time.…”

Section: X-ray Reflectivitymentioning

confidence: 99%

“…We have previously shown, using  casein -silica [4,5], that protein-silica binding can be readily detected through induced surface activity using x-ray and neutron surface reflectivity -since the reflectivity from the sol sub-phase for both types of radiation is very weak.…”

Section: Interaction Of Human Serum Albumin and Silica At The Air-watmentioning

confidence: 99%