2010
DOI: 10.1073/pnas.0914517107
|View full text |Cite
|
Sign up to set email alerts
|

Protein transduction from retroviral Gag precursors

Abstract: Retroviral particles assemble a few thousand units of the Gag polyproteins. Proteolytic cleavage mediated by the retroviral protease forms the bioactive retroviral protein subunits before cell entry. We hypothesized that this process could be exploited for targeted, transient, and dose-controlled transduction of nonretroviral proteins into cultured cells. We demonstrate that gammaretroviral particles tolerate the incorporation of foreign protein at several positions of their Gag or Gag-Pol precursors. Receptor… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

2
118
1

Year Published

2011
2011
2019
2019

Publication Types

Select...
7

Relationship

0
7

Authors

Journals

citations
Cited by 125 publications
(121 citation statements)
references
References 52 publications
2
118
1
Order By: Relevance
“…The protein of interest Protein transduction by LENA T Aoki et al can be fused to the C-terminus of Gag or inserted into the middle of the Gag protein (for example, between the MA and CA). 11,12 Foreign protein fusion to the C-terminus of Gag would destroy the frameshift signal required to produce Gag-pol. Thus, Pol would no longer be produced.…”
Section: Resultsmentioning
confidence: 99%
See 2 more Smart Citations
“…The protein of interest Protein transduction by LENA T Aoki et al can be fused to the C-terminus of Gag or inserted into the middle of the Gag protein (for example, between the MA and CA). 11,12 Foreign protein fusion to the C-terminus of Gag would destroy the frameshift signal required to produce Gag-pol. Thus, Pol would no longer be produced.…”
Section: Resultsmentioning
confidence: 99%
“…These data show that a protein transduction system based on LENA would have an extra level of safety compared with the protein transduction system based on a retroviral vector system. 11 Protein transduction in a broad sense, referring to the transport of protein across the cell membrane, is a useful technique in experimental molecular biology. It does not require de novo transcription and translation, and the transferred protein functions immediately after the transduction.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Gibbon ape leukemia virus (GALV) pseudotyped retroviral vector particles were produced by co-transfecting HEK 293T cells with the respective retroviral transfer plasmid together with packaging and envelope plasmids M634 (pcDNA3.MLVgp) 17 and M620 18 using polyethylenimine transfection as described elsewhere. 19 Vesicular stomatitis virus protein G (VSV-G) pseudotyped lentiviral vector particles were produced by cotransfecting HEK 293T cells with the respective lentiviral transfer plasmid together with packaging and envelope plasmids pCMVDR8.91 and pMD2.G.…”
Section: Transduction Of Tumor Cellsmentioning
confidence: 99%
“…In addition, there are a few reports where VLPs generated using nonstructural HIV proteins (e.g., Nef and Vpr) were used to deliver foreign proteins (25)(26)(27). Recently, Voelkel et al (28) reported that they were able to deliver proteins (GFP and functional Flp recombinase) to cells using murine retroviral particles. However, in all these cases, Gag-Pol was used to generate the VLPs.…”
mentioning
confidence: 99%