Protein synthesize function of bulls liver at experimental chronic cadmium toxicity

Abstract

The article presents the results of studies on the influence of cadmium load on the body of young cattle. The adverse effect of Cadmium on the liver causes a wide range of pathological changes at various levels of its organization. The purpose of the work was to investigate protein synthesize function on the liver of bulls for experimental chronic cadmium toxicity. The research was carried out on the basis of a farm at the village Ivanivtsi, Zhydachiv district of Lviv region, on 10 bulls of six months old, Ukrainian black-and-white milk breed, which were formed in 2 groups of 5 animals in each: control and experimental. Bulls of the control group were in the usual diet. Bulls of the experimental group were fed with forage with cadmium chloride at a dose of 0.04 mg/kg body weight of the animal. The research lasted for 30 days. Protein synthesize function of the liver was determined by the level of serum total protein and protein fractions. It was established that when feeding cows with feed of cadmium chloride in a dose of 0.04 mg/kg of body weight of an animal for 30 days in animals suppressed protein synthesize function of the liver, which manifests itself by lowering the total protein of blood. It reached the lowest level at the 20th day of the experiment, where, accordingly, it fluctuated within the limits of 61.9 ± 1.14 g/l. Reducing the level of total protein in the blood of the bulls of the experimental group under cadmium loading was due to lowering the level of albumin. At day 20 of the experiment, the level of albumin in the blood of animals in the experimental group decreased by 16.5%. On the background of general hypoproteinemia there is a significant imbalance between albumins and globulins in serum of diseased animals. It was established that the level of globulins increased in the blood of Bulls, which were under cadmium load. Increasing the level of globulins in blood serum of bulls reflects the intensity of inflammatory processes in their body for cadmium loading.

The intensity of the processes of oxidative modification of proteins and the activity of enzymes of the antioxidant protection system of broiler chickens under the action of vitamins E and C

“…It is known that under conditions of oxidative stress and excessive generation of AEO, processes of uncontrolled protein modification is developing, that cause protein fragmentation, their denaturation, and also the formation of primary amino acid radicals, further engaging in secondary interaction with adjacent amino acid residues, and in general, it creates a rather complicated picture of the damaging action of AEO on protein macromolecules. All this leads to the loss of their biological activity by proteins and disruption of metabolic processes, in particular regenerative processes (Armstrong, 2002;Martyshuk et al, 2016;Lavryshyn et al, 2016;Gutyj et al, 2018;Lavryshyn & Gutyj, 2019).…”

Protein-synthesizing function and functional state of the liver of dogs at experimental toxocariasis

“…Важливе діагностичне значення за токсокарозної інвазії в собак має визначення протеїнсинтезувальної функції печінки, про яку вказує рівень загального протеїну, а саме його альбумінової фракції. Оскільки 80 % альбумінів синтезується гепатоцитами у печінці (Lavryshyn & Gutyj, 2019;Varkholiak & Gutyj, 2019;Lesyk et al, 2020;Chernushkin et al, 2020).…”

Імунний Статус Організму Бугайців За Умови Експериментального Хронічного Кадмієвого Токсикозу

“…Реакція імунної системи може бути на саму речовину, її метаболіти, комплексні антигени, що утворились в організмі при інтоксикації. Токсична дія Кадмію на імунну систему є неоднаковою за інтенсивністю та направлена на різні етапи імуногенезу [2,4,10,25].…”