Protein Synthesis Inhibition: Mechanism for the Production of Impaired Fat Absorption

Sabesin, Seymour M.; Isselbacher, Kurt J.

doi:10.1126/science.147.3662.1149

Cited by 145 publications

(45 citation statements)

References 13 publications

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“…Since lipoproteins To document further the impaired transport of have been shown to be essential for the transport of tripalmitin after ACH treatment, we carried out long chain triglycerides from intestinal mucosa studies in which samples of portal and aortic blood (8,9), experiments were carried out with ACH were obtained 60 minutes after injection of tripalto determine the effect of impaired protein synthe-mitin into intestinal loops. The lipids were fracsis on the mucosal uptake and transfer of '4C-tionated by thin layer chromatography and samples labeled lipids from intestinal loops (Table VII).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Absorption of medium and long chain triglycerides: factors influencing their hydrolysis and transport.

Greenberger¹,

Rodgers²,

Isselbacher³

1966

J. Clin. Invest.

189

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Recent clinical observations have indicated that triglycerides containing medium chain fatty acids may be useful in decreasing steatorrhea associated with malabsorptive states such as pancreatic insufficiency and extrahepatic biliary tract obstruction (1, 2). This suggests that significant amounts of medium chain triglycerides may be absorbed under conditions in which there are decreased intraluminal concentrations of pancreatic enzymes or bile salts. However, few quantitative data are available regarding the intestinal hydrolysis and the rate of absorption of medium chain triglycerides as compared to long chain triglycerides.We have compared the intestinal hydrolysis and absorption of a medium chain triglyceride (trioctanoin) with those of a long chain triglyceride (tripalmitin) under a variety of experimental conditions. These studies were performed with isolated intestinal loops in the rat.The data obtained indicate that there are significant differences in the rates of hydrolysis and absorption of trioctanoin compared to tripalmitin. We also observed that with decreased intraluminal concentrations of bile salts and pancreatic enzymes there was, as expected, decreased absorption of both trioctanoin and tripalmitin. However, under * Submitted for publication July 29, 1965; accepted October 29, 1965. This work was supported in part by grants from the John A. Hartford Foundation, Inc., and the National Institutes of Health (AM-01392, 03014, and T1-AM-5146).Part of this work was presented at the annual meeting of the American Federation for Clinical Research, Atlantic City, N. J., May 2, 1965, and appears in abstract form in Clin. Res. 1965, 13, 253. Samples of the various '4C-labeled lipid-albumin suspensions were added to 12 ml of a solution of p-dioxane containing 5%o naphthalene, 0.01% p-bis-2'-(5'-phenyloxazolyl)benzene, 0.7%o p-bis-2,5-diphenyloxazole, and 13.3%o absolute ethanol and were counted in a Packard Tri-Carb liquid scintillation spectrometer. The solution prepared for counting substances in organic solvents contained 15 ml of toluene with 4.0% Liquifluor.2 Quenching was corrected for by utilizing a standard quenching curve as described by Bush (3).Experiments on lipid absorption from isolated intestinal loops. With female rats weighing 160 to 180 g under light ether anesthesia, a laparotomy was performed, and a 20-to 30-cm segment of the lower duodenum and jejunum was isolated between double silk ligatures, care being taken not to obstruct the blood flow. In experiments in which normal intestinal loops were-utilized, the proximal ligature was placed at the pyloroduodenal

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Section: Resultsmentioning

confidence: 99%

“…The proteins of the chylomicron are probably those synthesized within the intestinal mucosa (8). It has been demonstrated that after the administration of agents such as puromycin and ethionine, which inhibit protein and lipoprotein synthesis, the transport of long chain triglycerides is markedly impaired (9)(10)(11).…”

Section: Discussionmentioning

confidence: 99%

Absorption of medium and long chain triglycerides: factors influencing their hydrolysis and transport.

Greenberger¹,

Rodgers²,

Isselbacher³

1966

J. Clin. Invest.

189

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“…the enterocytes involved were characterized by their low natural capacity to synthesize lipoproteins and to export them. It should also be mentioned that experimental inhibition of protein synthesis in mammals results m an enlargement of enterocytic chylomicrons (Glickman et al 1972) and to extensive development of l~p i d droplets (Sabesin & Isselbacher 1965, Friedman & Cardell 1972.…”

Section: Discussionmentioning

confidence: 99%

“…The purpose of the present work on sea bass Dicentrarchus labrax, a carnivorous fish with stomach, was to study in detail the deterioration of the proximal intestine, which is the site of lipid absorption, and to relate it as far as possible to disturbances in this phase of digestion. Many studies in mammals, particularly in humans, have shown links between histopathologies of the intestinal epithelium and natural or induced disturbances in fat digestion and absorption (Sabesin & Isselbacher 1965, Dobbins 1966, Shimoda et al 1968, Rubin 1971, Ament et al 1972, Friedman & Cardell 1977, Takahashi & Mizunuma 1984.…”

Section: Introductionmentioning

confidence: 99%

Intestinal steatosis in the farm-reared sea bass Dicentrarchus labrax

Deplano¹,

Connes²,

Diaz³

et al. 1989

Dis. Aquat. Org.

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Light microscopy, scanning and transmission electron microscopy were used to study the anterior intestine of the sea bass Dicentrarchus labrax L., involved in lipid absorption. In the first part of the study, intestinal morphology of control feral fishes was described and lipid absorption through the intestinal mucosa was defined by comparing starved and fed individuals. In the second part, similar investigations were made on specimens reared on artificial food. These show injuries of the mucosa, ranging from simple impairment of enterocytic striated border to cellular necrosis and abrasion. The severity of injuries was related to the extent of lipid overloading within the mucosa. This overloadng was mainly represented by abundant lipid droplets in epithelial cells, and by the accumulation of numerous and large lipid granules in interenterocytic spaces and in the lamina propria. Possible influences of artificial feed on this digestive pathology are discussed from quantitative and qualitative points of view.

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“…In this regard, Isselbacher and Budz (1963), and Sabesin and Isselbacher (1965) tested the effect of known protein synthe sis inhibitors (puromycin, acetoxycycloheximide, and ethionine) on rats in vivo. Results were similar to those of the abetalipoproteinemia disorder in that there occurred an accumulation of triglycerides in intestinal cells, and a decrease in production of g-lipoprotein and in incorporation of C^^-leucine into protein.…”

Section: Intracellular Phasementioning

confidence: 99%

The ultrastructure of lipid absorption and transport in bovine intestinal mucosa

Sterzing

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Protein Synthesis Inhibition: Mechanism for the Production of Impaired Fat Absorption

Cited by 145 publications

References 13 publications

Absorption of medium and long chain triglycerides: factors influencing their hydrolysis and transport.

Absorption of medium and long chain triglycerides: factors influencing their hydrolysis and transport.

Intestinal steatosis in the farm-reared sea bass Dicentrarchus labrax

The ultrastructure of lipid absorption and transport in bovine intestinal mucosa

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