Protein Synthesis in the Rat Anterior Pituitary: II. Solubility Studies on Total Protein, Growth Hormone and Prolactin Labeled in an<i>in Vitro</i>Incubation

Samli, Marcqueta H.; Lai, Minchao; Barnett, Charlotte A.

doi:10.1210/endo-91-1-227

Cited by 4 publications

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“…Our results are in excellent agreement with those of Jones et al (5) who eluted these proteins from the polyacrylamide gels and established their identity by bioassay. In addition, the excellent separation of rat growth hormone and prolactin by this method has been described by other workers (7)(8)(9)(10).…”

mentioning

confidence: 76%

Effect of Anti-mitotic Drugs on the in Vitro Secretory Activity of Mammotrophs and Somatotrophs and on Their Microtubules

MacLeod

Lehmeyer

Bruni

1973

Experimental Biology and Medicine

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confidence: 76%

Effect of Anti-mitotic Drugs on the in Vitro Secretory Activity of Mammotrophs and Somatotrophs and on Their Microtubules

MacLeod

Lehmeyer

Bruni

1973

Experimental Biology and Medicine

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The Biosynthesis of Prolactin

Maurer

Stone

Gorski

1978

Structure and Function of the Gonadotropins

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Hormone Secretion by Cells Dissociated From Rat Anterior Pituitaries

Hopkins

Farquhar

1973

The Journal of Cell Biology

240

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A new procedure has been developed for dissociating anterior pituitary tissue and producing a viable suspension of single cells . The procedure involves incubation of small tissue blocks in 1 mg/ml trypsin (15 min), followed by incubation in 8µg/ml neuraminidase and 1 mM EDTA (15 min), followed by mechanical dispersion . Cell yields are -55%, based on recovered DNA. By electron microscopy five types of secretory cells (somatotrophs, mammotrophs, thyrotrophs, gonadotrophs, and corticotrophs) plus endothelial and follicular cells can be identified and are morphologically well preserved up to 20 h after dissociation . Throughout this period, the cells incorporate linearly [ 3 H]leucine into protein for up to 4 h at a rate 90% greater than hemipituitaries, and they synthesize, transport intracellularly, and release the two major pituitary secretory products, growth hormone and prolactin . Immediately after dissociation the cells' ability to respond to secretogogues (high K+ and dibutyryl cyclic AMP) is impaired, but after a 6-12-h culture period, the cells apparently recover and discharge 24% and 52%, respectively, of their content of prelabeled growth hormone over a 3-h period in response to these two secretogogues . This represents a stimulation of 109% and 470% over that released by cells incubated in control medium . The results demonstrate that function and morphologic integrity are preserved in this cell system . Therefore it is suitable for the study of various aspects of pituitary secretion and its control .

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Protein Synthesis in the Rat Anterior Pituitary: II. Solubility Studies on Total Protein, Growth Hormone and Prolactin Labeled in anin VitroIncubation

Cited by 4 publications

References 0 publications

Effect of Anti-mitotic Drugs on the in Vitro Secretory Activity of Mammotrophs and Somatotrophs and on Their Microtubules

Effect of Anti-mitotic Drugs on the in Vitro Secretory Activity of Mammotrophs and Somatotrophs and on Their Microtubules

The Biosynthesis of Prolactin

Hormone Secretion by Cells Dissociated From Rat Anterior Pituitaries

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