Protein synthesis in sperm: Dialog between mitochondria and cytoplasm

Gur, Yonatan; Breitbart, Haim

doi:10.1016/j.mce.2007.11.015

Cited by 108 publications

(80 citation statements)

References 77 publications

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“…When enrichment efficiency was compared, the percentage of acetylated peptides over total peptides ranged from ϳ12 to 31%, which was higher than that of polyclonal antibodies (10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)see Ref. 32).…”

Section: Discussionmentioning

confidence: 88%

“…Unlike somatic cells, mature sperm are almost transcriptionally and translationally silent except for minor translation of mRNAs by mitochondrial-type ribosomes (15), which requires that PTMs in sperm play more important roles in regulation by replacing the roles that could be burdened on transcriptional and translational machines in somatic cells. The importance of phosphorylation, an essential PTM in sperm differentiation, maturation, and fertilization, has been well established (16,17).…”

mentioning

confidence: 99%

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Acetylproteomic Analysis Reveals Functional Implications of Lysine Acetylation in Human Spermatozoa (sperm)

Diao

Wang

et al. 2015

Molecular & Cellular Proteomics

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Male infertility is a medical condition that has been on the rise globally. Lysine acetylation of human sperm, an essential posttranslational modification involved in the etiology of sperm abnormality, is not fully understood. Therefore, we first generated a qualified pan-anti-acetyllysine monoclonal antibody to characterize the global lysine acetylation of uncapacitated normal human sperm with a proteomics approach. With high enrichment ratios that were up to 31%, 973 lysine-acetylated sites that matched to 456 human sperm proteins, including 671 novel lysine acetylation sites and 205 novel lysine-acetylated proteins, were identified. These proteins exhibited conserved motifs XXXKYXXX, XXXKFXXX, and XXXKHXXX, were annotated to function in multiple metabolic processes, and were localized predominantly in the mitochondrion and cytoplasmic fractions. Between the uncapacitated and capacitated sperm, different acetylation profiles in regard to functional proteins involved in sperm capacitation, sperm-egg recognition, sperm-egg plasma fusion, and fertilization were observed, indicating that acetylation of functional proteins may be required during sperm capacitation. Bioinformatics analysis revealed association of acetylated proteins with diseases and drugs. Novel acetylation of voltage-dependent anion channel proteins was also found. With clinical sperm samples, we observed differed lysine acetyltransferases and lysine deacetylases expression between normal sperm and abnormal sperm of asthenospermia or necrospermia. Furthermore, with sperm samples impaired by epigallocatechin gallate to mimic asthenospermia, we observed that inhibition of sperm motility was partly through the blockade of voltage-dependent anion channel 2 Lys-74 acetylation combined with reduced ATP levels and mitochondrial membrane potential. Taken together, we obtained a qualified pananti-acetyllysine monoclonal antibody, analyzed the acetylproteome of uncapacitated human sperm, and revealed associations between functional protein acetylation and sperm functions. Molecular & Cellular

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Section: Discussionmentioning

confidence: 88%

mentioning

confidence: 99%

Acetylproteomic Analysis Reveals Functional Implications of Lysine Acetylation in Human Spermatozoa (sperm)

Diao

Wang

et al. 2015

Molecular & Cellular Proteomics

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show abstract

“…34 However, it has recently been demonstrated that there is protein translation in mature sperm. 35 The incorporation of labelled amino acids into polypeptides during sperm capacitation was completely inhibited by the mitochondrial translation inhibitor D-chloramphenicol but not by the cytoplasmic translation inhibitor cycloheximide, and inhibition of protein translation significantly reduced sperm motility, capacitation and in vitro fertilization rates. 36 Thus, to determine which proteins were translated and to examine the importance of translation during capacitation, we studied the changes in protein expression during capacitation and determined which proteins were newly translated by mitochondrial-type ribosomes using proteomic approaches.…”

Section: Functional Proteomics Of a Mouse Model Of Normal Spermatogenmentioning

confidence: 93%

Proteomics of spermatogenesis: from protein lists to understanding the regulation of male fertility and infertility

Huang¹,

Sha²

2010

Asian J Androl

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Proteomic technologies have undergone significant development in recent years, which has led to extensive advances in protein research. Currently, proteomic approaches have been applied to many scientific areas, including basic research, various disease and malignant tumour diagnostics, biomarker discovery and other therapeutic applications. In addition, proteomics-driven research articles examining reproductive biology and medicine are becoming increasingly common. The key challenge for this field is to move from lists of identified proteins to obtaining biological information regarding protein function. The present article reviews the available scientific literature related to spermatogenesis. In addition, this study uses two-dimensional electrophoresis mass spectrometry (2DE-MS) and liquid chromatography (LC)-MS to construct a series of proteome profiles describing spermatogenesis. This large-scale identification of proteins provides a rich resource for elucidating the mechanisms underlying male fertility and infertility.

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“…In the capacitationdependent process, nuclear-encoded mRNA can be translated by the mitochondrial type ribosomes whereas cytoplasmic machinery is not involved. In a recent viewpoint, mature sperm has a capacity of de novo protein translation (Gur & Breitbart 2008).…”

Section: V-atpase Induces Immune Responses Of Pregnancymentioning

confidence: 99%

V-ATPase upregulation during early pregnancy: a possible link to establishment of an inflammatory response during preimplantation period of pregnancy

Jaiswal¹,

Mallers²,

Larsen³

et al. 2012

Reproduction

104

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Various mechanisms exist to prevent a potentially deleterious maternal immune response that results in compromising survival of semiallogeneic fetus. In pregnancy, there is a necessary early preimplantation inflammatory stage followed by a postimplantation antiinflammatory stage. Thus, there is a biphasic 'immune response' observed during the course of pregnancy. We provide the evidence that capacitation of sperm induced the expression of a2 isoform of V-ATPase (ATP6V0A2 referred to as a2V), leukemia inhibitory factor (Lif), Il1b, and Tnf in the sperm. Capacitated sperm also released cleaved N-terminal domain of a2V-ATPase (a2NTD), which upregulates the gene expression of Lif, Il1b, Tnf, and monocyte chemotactic protein-1 (Ccl2 (Mcp1)) in the uterus. Unfertilized eggs had low a2V expression, but after fertilization, the expression of a2V increased in zygotes. This increased level of a2V expression was maintained in preimplantation embryos. Seminal plasma was necessary for upregulation of a2V expression in preimplantation embryos, as mating with seminal vesicle-deficient males failed to elicit an increase in a2V expression in preimplantation embryos. The infiltration of macrophages into the uterus was significantly increased after insemination of both sperm and seminal plasma during the preimplantation period of pregnancy. This dynamic infiltration into the uterus corresponded with the uterine a2V expression through the induction of Ccl2 expression. Furthermore, the polarization ratio of M1:M2 (pro-inflammatory/anti-inflammatory) macrophages in the uterus fluctuated from a ratio of 1.60 (day 1) to 1.45 (day 4) when female mice were inseminated with both sperm and seminal plasma. These data provide evidence that exposure to semen may initiate an inflammatory milieu by inducing a2V and cytokine/chemokine expression, which triggers the influx of macrophages into the preimplantation uterus during the onset of pregnancy and ultimately leads to successful pregnancy outcome.

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Protein synthesis in sperm: Dialog between mitochondria and cytoplasm

Cited by 108 publications

References 77 publications

Acetylproteomic Analysis Reveals Functional Implications of Lysine Acetylation in Human Spermatozoa (sperm)

Acetylproteomic Analysis Reveals Functional Implications of Lysine Acetylation in Human Spermatozoa (sperm)

Proteomics of spermatogenesis: from protein lists to understanding the regulation of male fertility and infertility

V-ATPase upregulation during early pregnancy: a possible link to establishment of an inflammatory response during preimplantation period of pregnancy

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