Protein synthesis in mitochondria from yeast strains carrying nam and mim suppressor genes

Zagórski, Włodzimierz; Kozłowski, Mirosław; Mieszczak, Maria; Spyridakis, Athanase; Claisse, Maurice; Słonimski, Piotr P.

doi:10.1016/0300-9084(87)90089-7

Cited by 10 publications

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“…Two nuclear mutants, nam3-1 and nam3-2, carrying a suppressor of the cob-M2001 mitochre mutation localized in the maturase coding region and preventing splicing of intron bi2 in the mitochondrial COB gene, were isolated (Kruszewska & Slonimski, 1984a). Genetic studies indicated an allele-specific, gene non-specific omnipotent mode of suppression that could result from decreased translation fidelity of premature stop codons (Kruszewska & Slonimski, 1984b;Zagorski et al, 1987b). This hypothesis was in part verified by direct biochemical data (Boguta et al, 1988;Mieszczak & Zagorski, 1987b).…”

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“…Genetic studies indicated an allele-specific, gene non-specific omnipotent mode of suppression that could result from decreased translation fidelity of premature stop codons (Kruszewska & Slonimski, 1984b;Zagorski et al, 1987b). This hypothesis was in part verified by direct biochemical data (Boguta et al, 1988;Mieszczak & Zagorski, 1987b). Recent studies identified nam3-1 and nam3-2 as alleles of the nuclear gene MRF1 encoding the mitochondrial release factor corresponding, respectively, to mrf1-145 352 S®I and mrf1-136 216 S®Y (Towpik et al, 2004).…”

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Translational readthrough of a termination codon in the yeast mitochondrial mRNA VAR1 as a result of mutation in the release factor mRF1.

Claisse

Boguta²,

Zagórski³

2005

Acta Biochim Pol

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Yeast mitochondrial DNA codes for eight major polypeptides. Translation of he mitochondrially encoded polypeptides in strains with mutated mitochondrial release factor, mRF1, was found to result in the synthesis of a novel protein, V2. Different mrf1 alleles were associated with different efficiency of V2p synthesis. Translation of V2p was enhanced by paromomycin. Comparative analysis of peptides resulting from protease digestion indicated that V2p is a derivative of Var1p. According to our hypothesis, V2p represents a readthrough product of the natural stop codon in VAR1 mRNA.An effective way of investigating the expression of genes in mtDNA is based on studying the control exerted by the nucleus over mitochondrial functions. This is due to replication, transcription and translation apparatus in mitochondria being composed mostly of polypeptides encoded by nuclear DNA. The yeast Saccharomyces cerevisiae, being a facultative anaerobe, offers distinct advantages for mitochondrial genetic studies. Several hundred respiratory deficient point mutations or small deletions, called mit -, have been genetically mapped and assigned to genes in mtDNA. Respiration of mitstrain Vol. 52 No. 1/2005 [129][130][131][132][133][134][135][136][137] QUARTERLY .

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confidence: 91%