Protein Synthesis in Chloroplasts

Preparation of Chlamydomonas Chloroplasts for the in Vitro Import of Polypeptide Precursors

“…Intact chloroplasts can thus be recovered which are competent for the in vitro import of radiolabeled precursor polypeptides (3,4). We have compared two approaches for their isolation: silicasol gradient centrifugation and elutriation (19,24).…”