Protein Synthesis Elongation Factor EF-1α is an Isopeptidase Essential for Ubiquitin-Dependent Degradation of Certain Proteolytic Substrates

Elongation factor-1 alpha is a selective regulator of growth factor withdrawal and ER stress-induced apoptosis

“…It is unclear whether enforced EF-1a expression can affect rates of protein translation. 18,25,26 We did not detect global effects on protein levels or rates of production. The lack of changes in global protein production does not rule out, however, that enduring EF-1a expression may alter the expression of individual proteins that protect the cell from apoptosis.…”

“…EF-1a is a GTPase that has multiple and divergent roles in cell physiology affecting the cytoskeleton, peptide synthesis and protein degradation. 18,22,23 We have found that the enforced expression of EF-1a can promote long-term viability from growth factor withdrawal-induced death in proportion to its level of expression. EF-1a protection does not extend to nuclear DNA damaging agents nor activation of TNF death receptors.…”

“…Manipulation of EF-1a protein levels has been reported to alter total cellular protein levels through mechanisms involving both protein synthesis and degradation. 18 Therefore, steady state protein levels and rates of protein synthesis were assessed. First, total cellular protein levels were compared.…”

RACK1 Function in Cell Motility and Protein Synthesis

“…These findings are consistent with the reported binding of eEF1A2 to damaged NSPs, 73,74 which was reported to promote their degradation by the proteasome. [75][76][77] Summary and Perspectives RACK1 is an abundant adaptor protein, and as such, it is not surprising that it participates in numerous cellular functions. Yet, recent data obtained from genetic RACK1 hypomorphic animals revealed distinct phenotypes associated with only 2 RACK1 functions: namely, its role in melanocyte biogenesis, which may be associated with cellular migration and movement of components of the neural crest lineage, and its clear role in mRNA translation.…”

Moonlighting Functions of Polypeptide Elongation Factor 1: From Actin Bundling to Zinc Finger Protein R1-Associated Nuclear Localization

“…Proteasome and Protein-disulˆde Isomerase Functions of EF-1a (EF-Tu) EF-1a and EF-Tu have been suggested to regulate 26S proteosome-dependent degradation of ubiquitinconjugated proteins. 143,144) Although the observations and speculation that``protein synthesis and degradation may be regulated by a common factor, EF-1a'', are interesting, these data and ideas have not yet been conˆrmed.…”