Protein Synthesis Directed by RNA Phage Messengers

Gussin, Gary N.; Capecchi, Mario R.; Adams, Jerry M.; Argetsinger, Joan E.; Tooze, John; Weber, Klaus-Josef; Watson, James D.

doi:10.1101/sqb.1966.031.01.034

Cited by 25 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Purification of the ribosomes had no effect on release, but purification of the supernatant led to a loss of release activity. In this system, an am ber mutation in the phage R17 RNA causes premature termination of the coat protein giving the small peptide fragment, fMet-Ala-Ser-Asn-Phe-Thr (274) . In this system, an am ber mutation in the phage R17 RNA causes premature termination of the coat protein giving the small peptide fragment, fMet-Ala-Ser-Asn-Phe-Thr (274) .…”

Section: Polypeptide Termination In Prokaryotementioning

confidence: 99%

Protein Biosynthesis

Lucas‐Lenard¹,

Lipmann²

1971

Annu. Rev. Biochem.

453

119

View full text Add to dashboard Cite

Section: Polypeptide Termination In Prokaryotementioning

confidence: 99%

Protein Biosynthesis

Lucas‐Lenard¹,

Lipmann²

1971

Annu. Rev. Biochem.

453

119

View full text Add to dashboard Cite

“…Another attractive feature of these phages is that their RNA can direct the synthesis of coat protein and RNA synthetase in cell-free systems, so that one can envisage the future possibility of following all stages of morphopoiesis in vitro (30,74,217).…”

Section: Self-assembly Of Biological Structuresmentioning

confidence: 99%

Self-assembly of biological structures.

Kushner¹

1969

Bacteriological Reviews

131

View full text Add to dashboard Cite

“…Translation initiation from the latter requires both the termination event of repY translation and the complementarity between 5Ј-side stem of structure III and the loop of structure I for the pseudoknot formation (8 -10). Yet, in the case of the MS2 RNA phage, a simple passage of ribosome through the coat gene region is sufficient to open the RBS for replicase and lysis proteins (26).…”

mentioning

confidence: 99%

“…It is likely that the balance between the affinity of the ribosome to each RBS and the stability of the secondary structure that blocks it make up this difference. In the case of MS2, the replicase and lysis protein RBSs are blocked with weak (bulged-out) secondary structures (26). Thus, the passage of the ribosome through the coat gene region may be sufficient to trigger ribosome binding to these RBSs and block the formation of RNA secondary structures.…”

mentioning

confidence: 99%

The Plasmid ColIb-P9 Antisense Inc RNA Controls Expression of the RepZ Replication Protein and Its Positive Regulator repYwith Different Mechanisms

Asano¹,

Hama²,

Inoue³

et al. 1999

Journal of Biological Chemistry

View full text Add to dashboard Cite

The autonomous replication region of plasmid ColIb-P9 contains repZ encoding the RepZ replication protein, and inc and repY as the negative and positive regulators of repZ translation, respectively. inc encodes the antisense Inc RNA, and repY is a short open reading frame upstream of repZ. Translation of repY enables repZ translation by inducing formation of a pseudoknot containing stem-loop I, which base pairs with the sequence preceding the repZ start codon. Inc RNA inhibits both repY translation and formation of the pseudoknot by binding to the loop I. To investigate control of repY expression by Inc RNA, we isolated a number of mutations that express repY in the presence of Inc RNA. One class of mutations delete a part of another stem-loop (II), which derepresses repY expression by initiating translation at codon 10 (GUG), located within this structure. Point mutations in stem-loop II can also derepress repY translation, and the introduction of compensatory basechanges restores control of repY translation. These results not only indicate that suppressing a cryptic start codon by secondary structure is important for maintaining the translational control of repZ but also demonstrate that the position of start site for repY translation is critical for its control by Inc RNA. Thus, Inc RNA controls repY translation by binding in the vicinity of the start codon, in contrast to the control of repZ expression at the level of loop-loop interaction.The initiation phase of translation is rate-limiting in protein synthesis and often the target of control of gene expression (1). It is well established that the selection of translational start sites in prokaryotes is stimulated by base pairing between the 3Ј-end of the small ribosomal RNA (16 S rRNA) and the Shine Dalgarno (SD) 1 sequence in the mRNA, located ϳ7 bases upstream of the initiation codon (2). The ribosome-binding site (RBS) of the mRNA contains the SD sequence and the initiation codon (3). Translational control can be achieved by sequestering or opening the RBS, either by binding of trans-acting factors (RNA or protein) or by modulating the higher-ordered structure of the mRNA (2). We have been studying the various aspects of translational control found in the replication control system in plasmid ColIb-P9 (4).The replication of the ColIb-P9 plasmid (93 kilobases, IncI␣ group) depends on expression of the replication initiator protein RepZ, encoded by its autonomous replication region (5), shown schematically in Fig. 1A. The level of repZ expression is strictly controlled at the translational level by the actions of positive and negative regulatory elements, repY (6) and inc (5, 7), respectively. repY is a short open reading frame encoding 29 amino acids, the 3Ј-end of which overlaps with the 5Ј-end of repZ (6) (see Fig. 1A). An amber mutation of repY codon 11 (rep57) did not allow repZ to be translated, indicating that repY translation is required for repZ translation (6). The process of repY translation and its termination, rather than the RepY polypeptide...

show abstract

Protein Synthesis Directed by RNA Phage Messengers

Cited by 25 publications

References 0 publications

Protein Biosynthesis

Protein Biosynthesis

Self-assembly of biological structures.

The Plasmid ColIb-P9 Antisense Inc RNA Controls Expression of the RepZ Replication Protein and Its Positive Regulator repYwith Different Mechanisms

Contact Info

Product

Resources

About