Protein–surfactant interactions at hydrophobic interfaces studied with total internal reflection fluorescence correlation spectroscopy (TIR-FCS)

Total internal reflection spectroscopy for studying soft matter

“…TIR-fluorescence correlation spectroscopy has been applied widely, for example to dyes at the solid-liquid interface, 132 the interaction of proteins and antibodies, 133 adsorption and binding to supported membranes, 131 , the adsorption and desorption kinetics of different dendrimers 134 and the competitive adsorption of proteins and surfactants. 135 An alternative approach is fluorescence recovery after photobleaching (FRAP or FPR). Here, surfacebound molecules are made non-fluorescent by photobleaching and the time required for the fluorescence intensity to recover is measured.…”

Biopolymer Surfactant Interactions

“…Surfactant solutions have a general tendency to solubilize a certain amount of additives, which can be correlated with their structural organization and mutual interactions. Polymer-surfactant interactions [1][2][3][4][5][6][7][8] have been extensively investigated by researchers due to its manifold applications in the fields of the food industry, pharmaceutical industry and analytical biochemistry. The interaction of protein with other ingredients, mostly cationic and anionic surfactants, is of particular interest because they are used co-operatively in formulated complexes.…”

An insight into the micellization of dodecyldimethylethylammonium bromide (DDAB) in the presence of bovine serum albumin (BSA)

“…In recent years, there has been growing interest in the interactions between ionic surfactants and water-soluble globular proteins because of their various industrial applications and inherently interesting properties [1][2][3][4][5][6][7]. This information can provide a fundamental concept for understanding the basic forces involved in protein self association.…”