Protein-Specific Analysis of Humoral Immune Responses in a Clinical Trial for Vaccines against Contagious Bovine Pleuropneumonia

Hamsten, Carl; Tjipura-Zaire, Georgina; Huebschle, Otto J.B.; Scacchia, Massimo; Ayling, Roger D.; Persson, Anja Bondke

doi:10.1128/cvi.00019-10

Cited by 12 publications

(10 citation statements)

References 35 publications

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“…The SC route of inoculation was used on the grounds of expediency (Davies et al 1968) but was discouraged due to doubts as to whether the results gave an accurate assessment of immunity. In-contact transmission method was later used extensively (Huebschle et al 2006;Niang et al 2006;Hamsten et al 2010;Totté et al 2010) because it approximates more closely to a natural infection (Provost et al 1987). However, the method employs an extra group of animals whose only role is to serve as donors of infective material, making it more expensive.…”

Section: Introductionmentioning

confidence: 99%

“…It is controlled mainly through vaccination, with other methods of control that include quarantine, restriction of movement, and stamping out policies. In the past, challenge of vaccinated cattle in experimental vaccination trials employed the subcutaneous (SC) route of inoculation (Piercy and Knight 1957) and later, in-contact transmission (Hamsten et al 2010) to measure efficacy of CBPP vaccines. The SC route of inoculation was used on the grounds of expediency (Davies et al 1968) but was discouraged due to doubts as to whether the results gave an accurate assessment of immunity.…”

Section: Introductionmentioning

confidence: 99%

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Assessing the effectiveness of intubation as a challenge model in contagious bovine pleuropneumonia vaccine experiments

Nkando

Wesonga

Kuria

et al. 2010

Trop Anim Health Prod

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A study was carried out to assess the effectiveness of a bronchoscope in administering a pathogenic field strain of Mycoplasma mycoides subsp. mycoides (MmmSC) in cattle challenge experiments. Out of 16 animals inoculated using the bronchoscope, 10 (62.2%) showed clinical disease as evidenced by fever and 15 (93.8%) displayed typical lesions of CBPP from which MmmSC was isolated. Serum samples collected weekly were tested by Complement Fixation Test (CFT) and competitive enzyme-linked immunosorbent assay (c-ELISA). Antibodies to MmmSC were detected in 10 out of the 16 animals by the CFT and 11 out of the 16 animals by c-ELISA. The onset of clinical disease was as early as 2 days post-inoculation, and most of the animals developed clinical disease 2 to 3 weeks post-infection. These results clearly demonstrate that nasotracheal inoculation of pathogenic strain of MmmSC with the aid of a bronchoscope can lead to early onset of clinical disease; similar to previous studies but with higher numbers of animals showing clinical disease. This is in contrast with previous studies where early clinical disease was observed in as little as 15% of inoculated animals. This nasotracheal inoculation method using a bronchoscope can, therefore, be adopted for use in experimental challenge infections of cattle. This method is found to be a better replacement to the contact transmission method whose drawback includes extra cost of donor animals and unpredictable rate and timing of transmission from intubated to challenge animals.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Assessing the effectiveness of intubation as a challenge model in contagious bovine pleuropneumonia vaccine experiments

Nkando

Wesonga

Kuria

et al. 2010

Trop Anim Health Prod

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“…Trotter et al [ 21 ] observed that antibodies produced against M. conjunctivae do not protect from re-infection. Similarly, vaccination attempts against mycoplasmoses did not protect from disease but even resulted in a worse disease course due to the artificially induced immune reaction [ 58 – 60 ]. By contrast, Baas et al [ 1 ] documented that sheep infected twice showed milder ocular signs the second than the first time, and overall, the protective role of an acquired immunity against M. conjunctivae remains controversial.…”

Section: Discussionmentioning

confidence: 99%

Infectious keratoconjunctivitis in wild Caprinae: merging field observations and molecular analyses sheds light on factors shaping outbreak dynamics

Gelormini

Gauthier²,

Vilei

et al. 2016

BMC Vet Res

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BackgroundInfectious keratoconjunctivitis (IKC) is an ocular infectious disease caused by Mycoplasma conjunctivae which affects small domestic and wild mountain ruminants. Domestic sheep maintain the pathogen but the detection of healthy carriers in wildlife has raised the question as to whether M. conjunctivae may also persist in the wild. Furthermore, the factors shaping the dynamics of IKC outbreaks in wildlife have remained largely unknown. The aims of this study were (1) to verify the etiological role of M. conjunctivae in IKC outbreaks recorded between 2002 and 2010 at four study sites in different regions of France (Pyrenees and Alps, samples from 159 Alpine ibex Capra ibex, Alpine chamois Rupicapra rupicapra and Pyrenean chamois Rupicapra pyrenaica); (2) to establish whether there existed any epidemiological links between the different regions through a cluster analysis of the detected strains (from 80 out of the 159 animals tested); (3) to explore selected pathogen, host and environmental factors potentially influencing the dynamics of IKC in wildlife, by joining results obtained by molecular analyses and by field observations (16,609 animal observations). All of the samples were tested for M. conjunctivae by qPCR, and cluster analysis was based on a highly variable part of the lppS gene.ResultsWe documented infections with M. conjunctivae in epidemic and endemic situations, both in symptomatic and asymptomatic animals. The identified M. conjunctivae strains were site-specific and persisted in the local wild population for at least 6 years. In epidemic situations, peaks of cases and disease resurgence were associated with the emergence of new similar strains in a given area. Social interactions, seasonal movements and the landscape structure such as natural and anthropogenic barriers influenced the spatio-temporal spread of IKC. Adults were more affected than young animals and host susceptibility differed depending on the involved strain.ConclusionOur study indicates that IKC is a multifactorial disease and that M. conjunctivae can persist in wildlife populations. The disease course in individual animals and populations is influenced by both host and mycoplasma characteristics, and the disease spread within and among populations is shaped by host behavior and landscape structure.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-017-0972-0) contains supplementary material, which is available to authorized users.

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“…Aside from subunit vaccine experiments, CBPP vaccine research has focused mainly on either live attenuated M. mycoides subsp. mycoides ( 7 , 39 , 40 ) or inactivated formulations of M. mycoides subsp. mycoides surface or total antigens ( 41 – 43 ).…”

Section: Discussionmentioning

confidence: 99%

Vaccination of Cattle with the N Terminus of LppQ of Mycoplasma mycoides subsp. mycoides Results in Type III Immune Complex Disease upon Experimental Infection

et al. 2015

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Contagious bovine pleuropneumonia (CBPP) is a serious respiratory disease of cattle caused by Mycoplasma mycoides subsp. mycoides. Current vaccines against CBPP induce short-lived immunity and can cause severe postvaccine reactions. Previous studies have identified the N terminus of the transmembrane lipoprotein Q (LppQ-N′) of M. mycoides subsp. mycoides as the major antigen and a possible virulence factor. We therefore immunized cattle with purified recombinant LppQ-N′ formulated in Freund's adjuvant and challenged them with M. mycoides subsp. mycoides. Vaccinated animals showed a strong seroconversion to LppQ, but they exhibited significantly enhanced postchallenge glomerulonephritis compared to the placebo group (P = 0.021). Glomerulonephritis was characterized by features that suggested the development of antigen-antibody immune complexes. Clinical signs and gross pathological scores did not significantly differ between vaccinated and placebo groups. These findings reveal for the first time the pathogenesis of enhanced disease as a result of antibodies against LppQ during challenge and also argue against inclusion of LppQ-N′ in a future subunit vaccine for CBPP.

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Protein-Specific Analysis of Humoral Immune Responses in a Clinical Trial for Vaccines against Contagious Bovine Pleuropneumonia

Cited by 12 publications

References 35 publications

Assessing the effectiveness of intubation as a challenge model in contagious bovine pleuropneumonia vaccine experiments

Assessing the effectiveness of intubation as a challenge model in contagious bovine pleuropneumonia vaccine experiments

Infectious keratoconjunctivitis in wild Caprinae: merging field observations and molecular analyses sheds light on factors shaping outbreak dynamics

Vaccination of Cattle with the N Terminus of LppQ of Mycoplasma mycoides subsp. mycoides Results in Type III Immune Complex Disease upon Experimental Infection

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