Protein sequences insight into heavy metal tolerance in Cronobacter sakazakii BAA-894 encoded by plasmid pESA3

Abstract

The recently annotated genome of the bacterium Cronobacter sakazakii suggests the organism has the ability to bind heavy metals. This study demonstrates heavy metal tolerance in Cronobacter sakazakii, in which proteins with the heavy metal interaction were recognized by computational and experimental study. As the result, approximately one fourth of proteins encoded on the plasmid pESA3 are proposed to have potential interaction with heavy metals. Interaction between heavy metals and predicted proteins was further corroborated using protein crystal structures from protein data bank database and comparison of metal-binding ligands. In addition with, a phylogenetic study was undertaken for most toxic heavy metals, like arsenic, cadmium, lead and mercury and obtained related tree pattern for lead, cadmium and arsenic. Laboratory studies confirmed the organism's tolerance to tellurite, copper and silver. These experimental and computational study data extend our understanding of the genes encoding for proteins of this important neonatal pathogen and provides further insights into the genotypes associated with features that can contribute to its persistence in the environment. The information will be of value for future environmental protection from heavy toxic metals.

The effect of β-glucan and its potential analog on the structure of Dectin-1 receptor

“…For this candidate ligand, it is required to have structural insights regarding best conformational docked structure and binding pose. MD simulation provides an effective means for predicting the preferred conformations of docking pose, revealed stability and binding insights with time simulation [31], further, contributes to the evaluation of new therapeutic concepts, identifies small chemical compounds and metal ions which were starting points for drug discovery [32,33].…”

Transcriptome-wide analysis reveals the progress of Cordyceps militaris subculture degeneration

“…Here, the expression of several genes involved in detoxification was up-regulated during strain degeneration, including that encoding streptothricin-acetyltransferase (CCM_00152), which degrades streptothricin [ 41 ]; gamma-glutamyltranspeptidase (CCM_02065), which reduces glutathione [ 41 ]; MFS multidrug transporter (CCM_02974), which confers resistance to antibiotics [ 42 ]; glutathione S-transferase (CCM_03461), which processes pesticides, heavy metals, fluoride and eliminating reactive oxygen species (ROS) [ 43 , 44 ]; 30 kDa heat shock protein (CCM_06821), which is involved in stress response, signal transduction, and xenobiotic compounds metabolism [ 45 ]; and alcohol dehydrogenase (CCM_09345), which ameliorates the harmful effects of high concentration of ethanol [ 46 ]. However, expression of the gene encoding heavy metal tolerance protein (CCM_06182), which binds heavy metals ions, was down-regulated during strain degeneration [ 47 ]. In addition, levels of non-nutritional xenobiotic compounds increased during strain degeneration induced by subculture.…”

DNA-Sequence Based Typing of the Cronobacter Genus Using MLST, CRISPR-cas Array and Capsular Profiling

“…A range of other virulence traits have been reported. These include Cronobacter plasminogen activator (cpa) ( Franco et al, 2011 ), iron utilization ( Grim et al, 2012 ), outer membrane vesicle release causing host cell cytopathogenicity ( Alzahrani et al, 2015 ; Kothary et al, 2017 ), as well as heavy metal resistance traits (copper, silver, zinc, tellurite) ( Joseph et al, 2012b ; Chaturvedi et al, 2015 ).…”