Protein separation under a microfluidic regime

Rodríguez-Ruiz, Isaac; Babenko, Vladislav V.; Martínez‐Rodríguez, Sergio; Gavira, José A.

doi:10.1039/c7an01568b

Cited by 28 publications

(22 citation statements)

References 218 publications

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“…The application of microfluidic to capillary electrophoresis for protein separation and analysis seems to be a topic of great interest, and it has been recently experimented and reviewed [22]. Different examples of analysis-on-chip are reported in literature.…”

Section: Microfluidic Technique Applicationsmentioning

confidence: 99%

The Microfluidic Technique and the Manufacturing of Polysaccharide Nanoparticles

et al. 2018

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The microfluidic technique has emerged as a promising tool to accelerate the clinical translation of nanoparticles, and its application affects several aspects, such as the production of nanoparticles and the in vitro characterization in the microenvironment, mimicking in vivo conditions. This review covers the general aspects of the microfluidic technique and its application in several fields, such as the synthesis, recovering, and samples analysis of nanoparticles, and in vitro characterization and their in vivo application. Among these, advantages in the production of polymeric nanoparticles in a well-controlled, reproducible, and high-throughput manner have been highlighted, and detailed descriptions of microfluidic devices broadly used for the synthesis of polysaccharide nanoparticles have been provided. These nanoparticulate systems have drawn attention as drug delivery vehicles over many years; nevertheless, their synthesis using the microfluidic technique is still largely unexplored. This review deals with the use of the microfluidic technique for the synthesis of polysaccharide nanoparticles; evaluating features of the most studied polysaccharide drug carriers, such as chitosan, hyaluronic acid, and alginate polymers. The critical assessment of the most recent research published in literature allows us to assume that microfluidics will play an important role in the discovery and clinical translation of nanoplatforms.

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Section: Microfluidic Technique Applicationsmentioning

confidence: 99%

The Microfluidic Technique and the Manufacturing of Polysaccharide Nanoparticles

et al. 2018

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“…These approaches have offered new information by creation of innovative conditions that are limited in conventional bulk methods. Microfluidic systems are being developed for applications in several areas such as protein purification and PCR on a drastically decreased scale ( 83 , 84 ). Microfluidic chips are capable of accurately replicating in vivo biological environments and allow high-throughput analysis of cells ( 85 ).…”

Section: Introduction: Immunoengineeringmentioning

confidence: 99%

Integrating Immunology and Microfluidics for Single Immune Cell Analysis

2018

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The field of immunoengineering aims to develop novel therapies and modern vaccines to manipulate and modulate the immune system and applies innovative technologies toward improved understanding of the immune system in health and disease. Microfluidics has proven to be an excellent technology for analytics in biology and chemistry. From simple microsystem chips to complex microfluidic designs, these platforms have witnessed an immense growth over the last decades with frequent emergence of new designs. Microfluidics provides a highly robust and precise tool which led to its widespread application in single-cell analysis of immune cells. Single-cell analysis allows scientists to account for the heterogeneous behavior of immune cells which often gets overshadowed when conventional bulk study methods are used. Application of single-cell analysis using microfluidics has facilitated the identification of several novel functional immune cell subsets, quantification of signaling molecules, and understanding of cellular communication and signaling pathways. Single-cell analysis research in combination with microfluidics has paved the way for the development of novel therapies, point-of-care diagnostics, and even more complex microfluidic platforms that aid in creating in vitro cellular microenvironments for applications in drug and toxicity screening. In this review, we provide a comprehensive overview on the integration of microsystems and microfluidics with immunology and focus on different designs developed to decode single immune cell behavior and cellular communication. We have categorized the microfluidic designs in three specific categories: microfluidic chips with cell traps, valve-based microfluidics, and droplet microfluidics that have facilitated the ongoing research in the field of immunology at single-cell level.

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“…The microfluidic scale has several advantages, such as lower consumption of reagents, separation speed and resolution, device portability, high repeatability, multiple assay etc. For this reason, many applications of microfluidic systems have been proposed so far, not only in chemical analysis but also in medical diagnosis, and the food and agricultural industries [4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Development of Microfluidic Components for Micro Total Analysis Systems

Naito

2020

Chromatography

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Microfluidic device is capable of reducing amount of sample consumption, shortening the analysis time, and miniaturizing instruments. Many applications of microfluidic devices have been developed not only in chemical analysis but also in medical diagnosis, and the food and agricultural industries. Electrophoresis or chromatography in a microfluidic device has improved the speed, reproducibility and separation resolution. Some of them have been integrated with complex experimental functionality on a small substrate, of which concept is known as micro total analysis systems. To build up a system, microfluidic components that carry out an experimental functionality in a microfluidic channel and novel technology to support the developments of microfluidic components are indispensable. In this review, three-dimensional (3D) fabrication by thiol-ene quick reaction, sample injection with an inkjet ejector, and molecular detection based on electroosmosis are focused briefly. The 3D fabrication realizes to make various 3D microstructures that conventional fabrication method cannot make without specific equipment. The sample injection by using inkjet technology can apply tiny amount of sample solution into multiple microfluidic channel on some precise spots, which leads to rapid analysis with high accuracy. The electroosmosis-based molecular detection indicates a possibility to develop a new portable device without any peripherals for detection or pretreatment for specimen labeling.

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Protein separation under a microfluidic regime

Cited by 28 publications

References 218 publications

The Microfluidic Technique and the Manufacturing of Polysaccharide Nanoparticles

The Microfluidic Technique and the Manufacturing of Polysaccharide Nanoparticles

Integrating Immunology and Microfluidics for Single Immune Cell Analysis

Development of Microfluidic Components for Micro Total Analysis Systems

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