Protein separation through preliminary experiments concerning pH and salt concentration by tube radial distribution chromatography based on phase separation multiphase flow using a polytetrafluoroethylene capillary tube

Kan, Hyo; Tsukagoshi, Kazuhiko

doi:10.1016/j.talanta.2017.03.075

Cited by 5 publications

(2 citation statements)

References 19 publications

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“…Phase separation is driven by multivalent interactions, which may be triggered by proteins containing intrinsically disordered regions (IDRs) 1,2 or by interactions with binding partners. 3,4 In addition, phase separation is regulated by active processes, including posttranslational modifications, [5][6][7] and is affected by environmental variables, such as temperature, 8 salt type and concentration, 9 and pH. 10,11 Phaseseparated condensates play an essential role in various biological processes, including the formation of heterochromatin, 12,13 gene expression, [14][15][16] cell division, 17,18 synaptic complex formation, [19][20][21][22][23][24][25] and autophagic degradation.…”

Section: Introductionmentioning

confidence: 99%

Liquid–liquid phase separation in synaptopathies

Jiang

2023

Neuroprotection

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Liquid–liquid phase separation has emerged as an important mechanism for regulating cell activity by promoting the formation of membrane‐less condensates. In the nervous system, the formation of distinct molecular condensates via phase separation is involved in synaptic assembly and function. Disruption of phase separation is associated with several disease states, such as cancer, neurodegenerative disorders, and neurodevelopmental disorders. This review summarizes the function of phase separation in the assembly and plasticity of the synapse, discusses the role of abnormal phase separation in neurological diseases, and proposes potential treatment options for these neurological diseases based on phase separation.

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Section: Introductionmentioning

confidence: 99%

Liquid–liquid phase separation in synaptopathies

Jiang

2023

Neuroprotection

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“…Novel open-tubular capillary chromatography has been performed based on specific TRDF; we named this separation method tube radial distribution chromatography (TRDC). [13][14][15] The outer phase in TRDF acts as a pseudo-stationary phase under laminar flow conditions during chromatographic separation. Recently we also studied TRDC from the viewpoint of the separation tube materials 16 as well as pressure loss on the separation performance.…”

Section: Introductionmentioning

confidence: 99%

Consecutive Sample Injection Analysis in Tube Radial Distribution Chromatography

et al. 2021

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Tube radial distribution chromatography based on the tube radial distribution flow, or annular flow, in an open-tubular capillary has been reported, where the annular flow is created through phase-separation multiphase flow. We have proposed the first-ever procedure for consecutive sample injection analysis using chromatography. In basic terms, a commercially available HPLC system could be used with a sample injector (0.2 μL volume) and a fused-silica capillary tube (250 cm long) as a separation column instead of a normal packed one, while the built-in detection cell was replaced by improved on-capillary detection. A ternary mixed solution of water/acetonitrile/ethyl acetate (3:8:4 volume ratio) was delivered into the capillary tube as an eluent at a flow rate of 2.0 μL min -1 . Model sample solutions of 1-naphthol and 2,6-naphthalenedisulfonic acid were consecutively analyzed by the present chromatography with a processing rate of 6 samples per hour. Simple and rapid consecutive analysis could be performed because washing and initialization of the separation tube was no longer necessary. The obtained results provide clues to developing new methodologies which combine features of both chromatography (separation) and the flow injection method (consecutive analysis).

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Phase-separation multiphase flow: preliminary application to analytical chemistry

Tsukagoshi

2023

ANAL. SCI.

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A two-phase separation mixed solution can undergo phase separation from one phase to two phases (i.e., upper and lower phases) in a batch vessel in response to changes in temperature and/or pressure. This phase separation is reversible. When the mixed solution undergoes a phase change while being fed into a microspace region, a dynamic liquid–liquid interface is formed, leading to a multiphase structure. This flow is called a phase-separation multiphase flow. Annular flow in a microspace, which is one such phase-separation multiphase flow, is interesting and has been applied to chromatography, extraction, reaction fields, and mixing. Here, research papers related to phase-separation multiphase flows—ranging from the discovery of the phenomenon to basic and technical research from the viewpoint of analytical science—are reviewed. In addition, the development of a new separation mode in a high-performance liquid chromatography system based on phase-separation multiphase flow is introduced. Graphical abstract

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Protein separation through preliminary experiments concerning pH and salt concentration by tube radial distribution chromatography based on phase separation multiphase flow using a polytetrafluoroethylene capillary tube

Cited by 5 publications

References 19 publications

Liquid–liquid phase separation in synaptopathies

Liquid–liquid phase separation in synaptopathies

Consecutive Sample Injection Analysis in Tube Radial Distribution Chromatography

Phase-separation multiphase flow: preliminary application to analytical chemistry

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