Protein-RNA crosslinking in the subunits of the cytoplasmic ribosome of Tetrahymena thermophila

Pétridou, Barbara; Guérin, M.F.; Hayes, F. Newton

doi:10.1016/0300-9084(89)90161-2

Cited by 6 publications

(1 citation statement)

References 44 publications

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“…act. 30 Tbq/mmol, 18.5 MBq/ml during 6-7 generations of exponential growth in a defined synthetic medium [3] as described in [4].…”

Section: Strains and Culture Conditionsmentioning

confidence: 99%

Ribosomal proteins of Tetrahymena thermophila. Correlation of one- and two-dimensional electrophoretic migration patterns and characterization of additional small and large subunit proteins

Pétridou¹,

Guérin²,

Hayes³

1989

Biochimie

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Further analysis of the protein complement of the cytoplasmic ribosome of the protozoon Tetrahymena thermophila has led to the identification and characterization of seven additional proteins, three in the small and four in the large subunit of this ribosome. Several of these proteins are poorly soluble or insoluble in the absence of high concentrations of urea and are not seen in the electrophoretic distribution patterns of ribosomal proteins in two-dimensional polyacrylamide gels unless 6 M urea is added to electrode buffers in contact with protein samples (first dimension) and first-dimension gels (second dimension). The migration patterns of the 40S and 60S subunits of the T. thermophila ribosome in one-dimensional polyacrylamide SDS gels and in two-dimensional gels prepared by means of the basic-acidic system of Kaltschmidt and Wittmann**, and the basic-SDS system of Zinker and Warner*** have been correlated.

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“…act. 30 Tbq/mmol, 18.5 MBq/ml during 6-7 generations of exponential growth in a defined synthetic medium [3] as described in [4].…”

Section: Strains and Culture Conditionsmentioning

confidence: 99%

Ribosomal proteins of Tetrahymena thermophila. Correlation of one- and two-dimensional electrophoretic migration patterns and characterization of additional small and large subunit proteins

Pétridou¹,

Guérin²,

Hayes³

1989

Biochimie

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Identification of proteins crosslinked to RNA in 40S ribosomal subunits of Saccharomyces cerevisiae

Xiang

Lee

1989

Biochimie

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Monovalent cation-dependent reversible phosphorylation of ribosomal protein S8 in growth arrested Tetrahymena: kinetics of formation, phosphoamino acids, and phosphopeptides of mono-, and diphosphorylated derivatives of protein S8

1991

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Protein-RNA crosslinking in the subunits of the cytoplasmic ribosome of Tetrahymena thermophila

Cited by 6 publications

References 44 publications

Ribosomal proteins of Tetrahymena thermophila. Correlation of one- and two-dimensional electrophoretic migration patterns and characterization of additional small and large subunit proteins

Ribosomal proteins of Tetrahymena thermophila. Correlation of one- and two-dimensional electrophoretic migration patterns and characterization of additional small and large subunit proteins

Identification of proteins crosslinked to RNA in 40S ribosomal subunits of Saccharomyces cerevisiae

Monovalent cation-dependent reversible phosphorylation of ribosomal protein S8 in growth arrested Tetrahymena: kinetics of formation, phosphoamino acids, and phosphopeptides of mono-, and diphosphorylated derivatives of protein S8

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