Protein restriction during puberty alters nutritional parameters and affects ovarian and uterine histomorphometry in adulthood in rats

Oliveira, Diego Augusto de Morais; Lupi, Luiz Antônio; Silveira, Henrique Spaulonci; Chuffa, Luiz Gustavo de Almeida

doi:10.1111/iep.12388

Cited by 6 publications

(5 citation statements)

References 42 publications

(61 reference statements)

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“…36 Mice with PD caused by consuming feed with a 6% protein content had a higher energy intake, thereby increasing the total fat weight and reducing lean body mass compared to normal mice consuming 20% protein. 37,38 A lack of protein intake causes increased hunger, resulting in a tendency to consume large amounts of food. Such occurs because proteins can create a longer-lasting feeling of fullness than carbohydrates and fats.…”

Section: Discussionmentioning

confidence: 99%

Bambara groundnut ameliorates kidney histology in female mice with protein deficiency

Firdiana,

Gunanegara,

Sunarti

et al. 2023

Med J Indones

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BACKGROUND Protein deficiency (PD) can lead to kidney damage. Consuming plant-based proteins may improve this condition. Bambara groundnut (Vigna subterranea)has an essential amino acid score of 80%, which is higher than other legumes; thus, it is potent in overcoming malnutrition. This study aimed to determine the effect of Bambara groundnut supplementation on kidney histology in adult female mice with PD. METHODS The study was conducted for 2 months in randomly selected female mice. These mice were grouped into the control, PD, and PD supplemented with Bambara groundnuts at 100, 200, and 300 g/kg of feed. 1 day after the last treatment, the kidneys of the mice were collected and processed histologically using the paraffin method (stained with hematoxylin and eosin and Masson’s trichrome). Parameters for observation included histopathological scoring (glomerular and interstitial space fibrosis and tubular damage), kidney histomorphometry, and organ index. Semi-quantitative data were analyzed using the Kruskal–Wallis test, while quantitative data were analyzed using one-way ANOVA (followed by Tukey’s test) and nested t-test. Statistical analysis was performed using SPSS software version 20 (IBM Corp., USA) (p≤0.05). RESULTS PD caused cell sloughing (moderate level) and dilatation (severe level) of the kidney tubules. It also reduced glomerular diameter and area by approximately 17.66% and 29%, respectively. PD and Bambara groundnut administration had no significant effects on the glomerular number, cortex and medulla thickness, distal and proximal tubule diameter, and kidney organ index (p>0.05). CONCLUSIONS Bambara groundnut (V. subterranea) administration prevented damage to the kidney’s histological structure of protein-deficient mice.

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Section: Discussionmentioning

confidence: 99%

Bambara groundnut ameliorates kidney histology in female mice with protein deficiency

Firdiana,

Gunanegara,

Sunarti

et al. 2023

Med J Indones

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“…In order to address this gap, we will compile and synthesise existing relevant studies in rodents to exemplify this aspect. In rats, nutritional protein restriction during puberty increased the oestrus phase arrest without changing the length of the oestrus cycle in adult females 20 . In addition, the low‐protein diet reduced the level of androgen receptor and elevated the oestrus receptor β in ovaries of rats 20 .…”

Section: Undernutrition and Human Reproductionmentioning

confidence: 98%

“…In rats, nutritional protein restriction during puberty increased the oestrus phase arrest without changing the length of the oestrus cycle in adult females. 20 In addition, the low-protein diet reduced the level of androgen receptor and elevated the oestrus receptor β in ovaries of rats. 20 Another study in rats showed that postnatal undernutrition delayed puberty onset.…”

Section: Postnatal/pubertal Undernutrition and Reproductionmentioning

confidence: 99%

“…20 In addition, the low-protein diet reduced the level of androgen receptor and elevated the oestrus receptor β in ovaries of rats. 20 Another study in rats showed that postnatal undernutrition delayed puberty onset. 21 Rats that were fed a proteinrestricted diet after weaning displayed reduced testis weight, spermatogonia, spermatocytes, spermatids, Leydig cells and the diameter of the seminiferous 2 of 10 -MODERN AGRICULTURE tubules.…”

Section: Postnatal/pubertal Undernutrition and Reproductionmentioning

confidence: 99%

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Balancing nutrition for successful reproduction in ruminants

Ding,

Liu,

Guan

et al. 2024

Modern Agriculture

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Proper nutrition is essential for sustaining optimal reproductive function in both humans and ruminants. Studies have shown that both undernutrition and overnutrition can negatively impact human reproductive processes. However, the Mediterranean diet, with its balanced and nutrient‐rich composition, is recognised for its positive effects on human reproduction. In the context of ruminants, a well‐balanced and nutrient‐rich diet is equally crucial for achieving peak reproductive performance. Grazing, which allows ruminants to feed on natural pasture in open environments, contrasts with indoor feeding, which is conducted in more controlled settings. A balanced dietary approach is vital for the successful reproduction of ruminant animals, and a combination of grazing and indoor feeding has been identified as an effective strategy to enhance their reproductive success. This review emphasises the impact of nutrition on reproductive outcomes in both human and ruminant populations, advocating for balanced dietary practices to improve reproductive success across species. A thorough understanding of the complex relationship between nutrition and reproductive health can offer valuable insights into enhancing fertility and reproductive efficiency in various contexts.

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“…11 However, as far as we known, there are no reports on the endothelial outcomes of protein restriction during the peripubertal period. The literature shows that protein restriction during puberty can cause metabolic 12,13 and reproductive 12,14 disorders in rats, demonstrating the importance of an adequate diet in the peripubertal period to homeostasis in adult life. In fact, it was recently described that protein restriction during peripubertal period can cause hypertension in adulthood, 15 however, as far as we know, the vascular function was not evaluated in this model.…”

Section: Introductionmentioning

confidence: 99%

Protein restriction during peripubertal period impairs endothelial aortic function in adult male Wistar rats

Souza

Silva

Jezuíno

et al. 2023

J Dev Orig Health Dis

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Protein restriction during early phases of body development, such as intrauterine life can favor the development of vascular disorders. However, it is not known if peripubertal protein restriction can favor vascular dysfunction in adulthood. The present study aimed to evaluated whether a protein restriction diet during peripubertal period favors endothelial dysfunction in adulthood. Male Wistar rats from postnatal day (PND) 30 until 60 received a diet with either 23% protein (CTR group) or with 4% protein (LP group). At PND 120, the thoracic aorta reactivity to phenylephrine, acetylcholine, and sodium nitroprusside was evaluated in the presence or absence of: endothelium, indomethacin, apocynin and tempol. The maximum response (Rmax) and pD2 (-log of the concentration of the drug that causes 50% of the Rmax) were calculated. The lipid peroxidation and catalase activity were also evaluated in the aorta. The data were analyzed by ANOVA (one or two-ways and Tukey’s) or independent t-test; the results were expressed as mean ± S.E.M., p < 0.05. The Rmax to phenylephrine in aortic rings with endothelium were increased in LP rats when compared with the Rmax in CTR rats. Apocynin and tempol reduced Rmax to phenylephrine in LP aortic rings but not in CTR. The aortic response to the vasodilators was similar between the groups. Aortic catalase activity was lower and lipid peroxidation was greater in LP compared to CTR rats. Therefore, protein restriction during the peripubertal period causes endothelial dysfunction in adulthood through a mechanism related to oxidative stress.

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Protein restriction during puberty alters nutritional parameters and affects ovarian and uterine histomorphometry in adulthood in rats

Cited by 6 publications

References 42 publications

Bambara groundnut ameliorates kidney histology in female mice with protein deficiency

Bambara groundnut ameliorates kidney histology in female mice with protein deficiency

Balancing nutrition for successful reproduction in ruminants

Protein restriction during peripubertal period impairs endothelial aortic function in adult male Wistar rats

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