Protein release from nanocellulose and alginate hydrogels: The study of adsorption and desorption kinetics

Lab-on-a-bead: Polymeric natural deep eutectic solvents as a versatile platform for (bio)sensor design

“…We hypothesize that these changes, which are somewhat in agreement with previous reports, [68][69][70] could be attributed to slight conformational changes in the enzymes, induced by the ALGPODES (or ALG) matrix, [71][72][73][74] that surrounds the proteins and (upon stabilization) creates a microenvironment that maintains their conformation and protects their active sites. 75 Considering that no color development was observed outside the beads, the release of the enzymes 76,77 or the redox dye to the solution is unlikely to be significant contributor to the decrease within the investigated timescale. Based on these results, ALGPODES were considered a superior platform for the development of the proposed biosensor and thus, used for the remaining experiments.…”