Protein Release from Galactoglucomannan Hydrogels: Influence of Substitutions and Enzymatic Hydrolysis by β-Mannanase

Roos, Alexandra A.; Edlund, Ulrica; Sjöberg, John; Albertsson, Ann‐Christine; Stålbrand, Henrik

doi:10.1021/bm701399m

Cited by 45 publications

(29 citation statements)

References 33 publications

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“…Figure 13 shows the cumulative release profiles as a function of release time for each BSA‐loaded hydrogel sample in PBS solution (pH 7.4, I = 0.2 M). It was found that there was a burst release at the initial stage, which was also observed in some other systems reported elsewhere 47, 72, 73. The BSA located near the hydrogel surface could be released rapidly from the hydrogel to the medium as soon as the sample was placed into the buffer solution.…”

Section: Resultssupporting

confidence: 81%

Synthesis and physicochemical characterization of biodegradable and pH‐responsive hydrogels based on polyphosphoester for protein delivery

Wang

et al. 2010

J. Polym. Sci. A Polym. Chem.

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In this work, a series of biodegradable and pHresponsive hydrogels based on polyphosphoester and poly (acrylic acid) are presented. A novel biodegradable macrocrosslinker a-methacryloyloxyethyl x-acryloyl poly(ethyl ethylene phosphate) (HEMA-PEOP-Ac) was synthesized by first ringopening polymerization of the cyclic monomer 2-ethoxy-2-oxo-1,3,2-dioxaphospholane using HEMA as the initiator and Sn(Oct) 2 as catalyst, and subsequent conversion of hydroxyl into vinyl group. The hydrogels were then fabricated by the copolymerization of the macromonomer with acrylic acid, and their swelling/ deswelling and degradation behaviors were investigated. The results demonstrated that the crosslinking density and pH values of media strongly influenced both the swelling ratio and the degradation rate of the hydrogels. The rheological properties of these hydrogels were also studied from which the storage modulus (G 0 ) showed clear dependence on the crosslinking density. MTT and ''live/dead'' assay showed that these hydrogels were compatible to fibroblast cells, not exhibiting apparent cytotoxicity even at high concentrations. Moreover, in vitro bovine serum albumin release from these hydrogels was also investigated, and it could be found that the release profiles showed a burst effect followed by a continuous release phase, and the release rate was inversely proportional to the crosslinking density of hydrogels.

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Section: Resultssupporting

confidence: 81%

Synthesis and physicochemical characterization of biodegradable and pH‐responsive hydrogels based on polyphosphoester for protein delivery

Wang

et al. 2010

J. Polym. Sci. A Polym. Chem.

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“…Polymers used in the development of CDDS can be chemically modified, and these modifications can influence the extent of enzymatic degradation. For example, Roos et al synthesized the acetyl derivative of guar gum (AcGGM) and used this polymer to make a hydrogel of bovine serum albumin (BSA) (46). They observed that the rate of hydrolysis for modified AcGGM by β-mannase was affected by its degree of substitution (DS).…”

Section: Colon-specific Biodegradable Delivery Systemsmentioning

confidence: 99%

Colon-Targeted Oral Drug Delivery Systems: Design Trends and Approaches

2015

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Abstract. Colon-specific drug delivery systems (CDDS) are desirable for the treatment of a range of local diseases such as ulcerative colitis, Crohn's disease, irritable bowel syndrome, chronic pancreatitis, and colonic cancer. In addition, the colon can be a potential site for the systemic absorption of several drugs to treat non-colonic conditions. Drugs such as proteins and peptides that are known to degrade in the extreme gastric pH, if delivered to the colon intact, can be systemically absorbed by colonic mucosa. In order to achieve effective therapeutic outcomes, it is imperative that the designed delivery system specifically targets the drugs into the colon. Several formulation approaches have been explored in the development colon-targeted drug delivery systems. These approaches involve the use of formulation components that interact with one or more aspects of gastrointestinal (GI) physiology, such as the difference in the pH along the GI tract, the presence of colonic microflora, and enzymes, to achieve colon targeting. This article highlights the factors influencing colon-specific drug delivery and colonic bioavailability, and the limitations associated with CDDS. Further, the review provides a systematic discussion of various conventional, as well as relatively newer formulation approaches/technologies currently being utilized for the development of CDDS.

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“…In addition to use in wound management, gels based on xyloglucans have been explored as vehicles for controlled drug delivery (Miyazaki et al, 1998;Roos et al, 2008). Research has shown that alkenyl-functionalized galactogluocomannans could offer sustained delivery of incorporated substances mediated by diffusion and enzymatic hydrolysis.…”

Section: Applications Of Hemicellulosesmentioning

confidence: 99%